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Rat C1q ELISA Kit (Colorimetric)

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ELISA: Rat C1q ELISA Kit (Colorimetric) [NBP2-74988] - Samples were spiked with high concentrations of Rat C1q and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat C1q ELISA Kit (Colorimetric) [NBP2-74988] - Samples were spiked with high concentrations of Rat C1q and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the ...read more
ELISA: Rat C1q ELISA Kit (Colorimetric) [NBP2-74988] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL

Order Details

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Rat C1q ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat C1 in samples. No significant cross-reactivity or interference between Rat C1 and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.23%
Intra-Assay
CV% < 4.82%
Spike Recovery
88-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
C1QA

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using
NBP2-74988 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat C1q ELISA Kit (Colorimetric)

  • A chain
  • complement component 1, q subcomponent, A chain
  • complement component 1, q subcomponent, alpha polypeptide

Background

C1q encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for C1q ELISA Kit (NBP2-74988)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA.


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Product General Protocols

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FAQs for C1q ELISA Kit (NBP2-74988). (Showing 1 - 2 of 2 FAQ).

  1. I am looking for polyclonal/monoclonal antibodies against canine C1q and complement-related. I was wondering if your prooduct does.
    • Please see the provided link below which will take you to our website and all of our C1Q antibodies that are available. Unfortunately we do not currently have an antibody that has been tested in dog but if that would be something you are interested in doing, I would like to invite you to review our Innovator's rewards program. Should you have any questions or concerns, please do not hesitate to contact me directly. C1Q antibodies: Innovators Rewards Program
  2. For NBP2-74988, I would like to measure C1q in rat serum samples. I understand it is necessary to determine the optimal sample dilutions for my particular experiments myself, but if you have any information from when you validated rat serum samples, could you share the recommended dilution of normal rat serum samples?
    • We have validated this kit with rat serum, and the samples were diluted 40000-80000 fold (200-800ug/mL). 

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Contact Information

Product PDFs

  SDS
  Rat C1q ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol C1QA