Biological Strategies: Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - HeLa cells mock (left) and treated with 50 uM BrdU for 24 hr (right) were fixed in 4% ...read more
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Mouse intestinal tissue pulsed with BrdU. BrdU stained by BrdU antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Biological Strategies: Flow Cytometry: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - BrdU antibody detects BrdU protein from HeLa treated with 10 uM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4C for ...read more
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified
Alternate Names for Bromodeoxyuridine/BrdU Antibody
5-bromo-2-deoxyuridine
BrdU
Bromodeoxyuridine
BUdr
Background
Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Bromodeoxyuridine/BrdU Antibody (NBP2-14890)(9)
We have publications tested in 2 confirmed species: Mouse, Rat.
We have publications tested in 4 applications: Electron Microscopy, ICC/IF, IHC, IHC-P.
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Electron Microscopy
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Showing Publications 1 - 9 of 9.
Publications using NBP2-14890
Applications
Species
Kim J, Kim S, Lee S et al. Alliin Enriched Standardized Fraction of Allium Hookeri Regulates the Glucose Homeostasis Via Upregulating Hepatic Glutathione Pool bioRxiv 2023-02-26 (IHC, Mouse)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Bromodeoxyuridine/BrdU Antibody (NBP2-14890). (Showing 1 - 1 of 1 FAQs).
I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
BrDU (Bromodeoxyuridine) The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2). Because BrDU is only incorporated in... Read full blog post.
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![Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - HeLa cells mock (left) and treated with 50 uM BrdU for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU protein stained by BrdU antibody. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14890-img0008.jpg "Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - HeLa cells mock (left) and treated with 50 uM BrdU for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU protein stained by BrdU antibody. Blue: Hoechst 33342 staining. Scale bar = 10 um")
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Mouse intestinal tissue pulsed with BrdU. BrdU stained by BrdU antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Immunohistochemistry-Paraffin-NBP2-14890-img0009.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Mouse intestinal tissue pulsed with BrdU. BrdU stained by BrdU antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Flow Cytometry: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - BrdU antibody detects BrdU protein from HeLa treated with 10 uM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4C for 15 minutes by flow cytometry analysis.Black: Unlabelled sample was used as a control.Red: BrdU antibodyAcquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Flow-Cytometry-NBP2-14890-img0004.jpg "Flow Cytometry: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - BrdU antibody detects BrdU protein from HeLa treated with 10 uM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4C for 15 minutes by flow cytometry analysis.Black: Unlabelled sample was used as a control.Red: BrdU antibodyAcquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")