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Bromodeoxyuridine/BrdU Antibody (BU-1) [Unconjugated]

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Bromodeoxyuridine/BrdU was detected in immersion fixed MCF-7 human breast cancer cell line stimulated with BrdU using Mouse Anti-Bromodeoxyuridine/ BrdU Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB7225) ...read more
Bromodeoxyuridine/BrdU was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Bromodeoxyuridine/BrdU Monoclonal Antibody (Catalog # MAB7225) at 1 µg/mL for 1 ...read more
Bromodeoxyuridine/BrdU was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Bromodeoxyuridine/BrdU Monoclonal Antibody (Catalog # MAB7225) at 1 µg/mL for 1 hour at room ...read more
Human peripheral blood mononuclear cells (PBMCs) were treated overnight with 50 ng/mL PMA, 500 ng/mL Ionomycin, and 30 µg/mL BrdU, then stained with Mouse Anti-Bromodeoxyuridine/BrdU Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications IHC, CyTOF-ready, Flow, ICC/IF
Clone
BU-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Bromodeoxyuridine/BrdU Antibody (BU-1) [Unconjugated] Summary

Immunogen
5-iodourindine (5-IO) coupled to ovalbumin
Specificity
Detects Bromodeoxyuridine/BrdU.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB7225 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bromodeoxyuridine/BrdU Antibody (BU-1) [Unconjugated]

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr

Background

Bromodeoxyuridine (BrdU) is a nucleoside analog that is incorporated into DNA in place of thymidine. The detection of newly synthesized DNA containing BrdU is a commonly used measure of cell proliferation and progression through S phase of the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bromodeoxyuridine/BrdU Antibody (MAB7225)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC, IF, Immunocytochemistry, Neutralization.


Filter By Application
ICC
(1)
IF
(1)
Immunocytochemistry
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB7225 Applications Species
Zhuo, H;Hou, J;Hong, Z;Yu, S;Peng, H;Zhang, L;Xie, W;Hong, X; TAGLN2 induces resistance signature ISGs by activating AKT-YBX1 signal with dual pathways and mediates the IFN-related DNA damage resistance in gastric cancer Cell death & disease 2024-08-21 [PMID: 39168971] (Immunocytochemistry, Human) Immunocytochemistry Human
DS Varghese, TT Alawathugo, MA Sheikh, AK Challagand, BS Emerald, SA Ansari Developmental modeling of hepatogenesis using obese iPSCs-hepatocyte differentiation uncovers pathological features Cell Death & Disease, 2022-08-01;13(8):670. 2022-08-01 [PMID: 35915082] (IF, Human) IF Human
Qilong Li, Quan Yuan, Ning Jiang, Yiwei Zhang, Ziwei Su, Lei Lv et al. Dihydroartemisinin regulates immune cell heterogeneity by triggering a cascade reaction of CDK and MAPK phosphorylation Signal Transduction and Targeted Therapy 7/11/2022 [PMID: 35811310]
Miles A. Tanner, Laurel A. Grisanti A Dual Role for Death Receptor 5 in Regulating Cardiac Fibroblast Function Frontiers in Cardiovascular Medicine 8/30/2021 [PMID: 34527710]
M Paku?a, E Ma?y, P Uruski, A Witucka, M Bogucka, N Jaroszewsk, N Makowska, A Niklas, R Moszy?ski, S Sajdak, A Tykarski, J Miku?a-Pie, K Ksi??ek Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells Cancers (Basel), 2020-01-27;12(2):. 2020-01-27 [PMID: 32012719] (Neutralization, Human) Neutralization Human
PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation. J. Cell. Sci., 2012-02-17;125(0):2276-87. 2012-02-17 [PMID: 22344267] (ICC, Human) ICC Human

Reviews for Bromodeoxyuridine/BrdU Antibody (MAB7225) (0)

There are no reviews for Bromodeoxyuridine/BrdU Antibody (MAB7225). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
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FAQs for Bromodeoxyuridine/BrdU Antibody (MAB7225). (Showing 1 - 1 of 1 FAQs).

  1. I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
    • We recommend this product: NB500-235​

Secondary Antibodies

 

Isotype Controls

Additional Bromodeoxyuridine/BrdU Products

Blogs on Bromodeoxyuridine/BrdU.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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