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Bassoon Antibody (SAP7F407) [HRP]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
SAP7F407
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-13249AF350
Alexa Fluor 532
NB120-13249AF532
Alexa Fluor 594
NB120-13249AF594
Alexa Fluor 750
NB120-13249AF750
Biotin
NB120-13249B
DyLight 550
NB120-13249R
DyLight 594
NB120-13249DL594
DyLight 650
NB120-13249C
FITC
NB120-13249F
Janelia Fluor 549
NB120-13249JF549
Janelia Fluor 646
NB120-13249JF646
Unconjugated
NB120-13249
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Bassoon Antibody (SAP7F407) [HRP] Summary

Immunogen
Recombinant rat Bassoon.
Specificity
This affinity purified detects an ~400 kDa protein, corresponding to the apparent molecular mass of Bassoon on SDSPAGE immunoblots, in samples from mouse and rat origins. Additional bands between 97 and 400 kDa may also be detected.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BSN
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Bassoon Antibody (SAP7F407) [HRP]

  • bassoon (presynaptic cytomatrix protein)

Background

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bassoon Antibody
(SAP7F407) [HRP]
NB120-13249H
Bassoon Antibody (SAP7F407) [HRP]
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bassoon Antibody (NB120-13249H). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?
    • After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BSN