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Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

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ELISA: Mouse Aminomethyltransferase ELISA Kit (Colorimetric) [NBP2-68202] - Samples were spiked with high concentrations of Mouse Aminomethyltransferase and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Mouse Aminomethyltransferase ELISA Kit (Colorimetric) [NBP2-68202] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

Order Details

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Mouse Aminomethyltransferase ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse AMT in samples. No significant cross-reactivity or interference between Mouse MAT and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.25%
Intra-Assay
CV% < 5.61%
Spike Recovery
85-100%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AMT

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

  • aminomethyltransferase
  • EC 2.1.2.10
  • GCE
  • GCSTaminomethyltransferase (glycine cleavage system protein T)
  • glycine cleavage system protein T
  • NKHmitochondrial

Background

The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AMT