![Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody (M01), clone 2F9-1F10.Lane 1: ORM1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Western-Blot-H00005004-M01-img0013.jpg "Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody (M01), clone 2F9-1F10.Lane 1: ORM1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP, KD |
| Clone | 2F9-1F10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | ORM1 (AAH26238, 18 a.a. ~ 201 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AQIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES |
| Specificity | ORM1 - orosomucoid 1 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ORM1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Publication using H00005004-M01 | Applications | Species |
|---|---|---|
| Hyung SW, Lee MY, Yu JH et al. A serum protein profile predictive of the resistance to neoadjuvant chemotherapy in advanced breast cancers. Mol Cell Proteomics. 2011-07-28 [PMID: 21799047] |
Secondary Antibodies |
Isotype Controls |
Research Areas for alpha 1-Acid Glycoprotein Antibody (H00005004-M01)Find related products by research area.
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![Immunohistochemistry-Paraffin: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Immunohistochemistry-Paraffin-H00005004-M01-img0008.jpg "Immunohistochemistry-Paraffin: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. Antibody concentration 1 ug/ml.")
![Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi ( Cat # H00005004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody (M01), clone 2F9-1F10 (Cat # H00005004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Western-Blot-H00005004-M01-img0010.jpg "Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi ( Cat # H00005004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody (M01), clone 2F9-1F10 (Cat # H00005004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - ORM1 monoclonal antibody (M01), clone 2F9-1F10 Analysis of ORM1 expression in MCF-7.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Western-Blot-H00005004-M01-img0012.jpg "Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - ORM1 monoclonal antibody (M01), clone 2F9-1F10 Analysis of ORM1 expression in MCF-7.")
![Immunoprecipitation: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 transfected lysate using anti-ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Immunoprecipitation-H00005004-M01-img0009.jpg "Immunoprecipitation: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 transfected lysate using anti-ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 MaxPab rabbit polyclonal antibody.")
![ELISA: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Detection limit for recombinant GST tagged ORM1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-ELISA-H00005004-M01-img0014.jpg "ELISA: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Detection limit for recombinant GST tagged ORM1 is approximately 0.1ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")