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Recombinant Human ADI1 His Protein

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SDS-Page: ADI1 Protein [NBP2-22667]
SDS-Page: ADI1 Protein [NBP2-22667]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human ADI1 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-179 of Human ADI1

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSMVL AWYMDDAPGD PRQPHRPDPG RPVGLEQLRR LGVLYWKLDA DKYENDPELE KIRRERNYSW MDIITICKDK LPNYEEKIKM FYEEHLHLDD EIRYILDGSG YFDVRDKEDQ WIRIFMEKGD MVTLPAGIYH RFTVDEKNYT KAMRLFVGEP VWTAYNRPAD HFEARGQYVK FLAQTA
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ADI1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
25.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human ADI1 His Protein

  • 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
  • Acireductone dioxygenase (Ni(2+)-requiring)
  • acireductone dioxygenase 1
  • APL1
  • ARDsubmergence induced protein 2
  • EC 1.13.11.53
  • FLJ10913
  • HMFT1638
  • membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1
  • Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1
  • MTCBP1
  • MTCBP-1
  • Ni-ARD
  • SIPLMT1-MMP cytoplasmic tail-binding protein-1
  • Submergence-induced protein 2 homolog

Background

ADI1 is an enzyme that belongs to the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Also, it down-regulates cell migration mediated by MMP14. Recombinant human ADI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol ADI1