Western Blot: 5-MethylCytosine Antibody [NB100-744] - Global epigenetic changes in HFFs 7 days after hESC extract treatment. (B) Immunoblotting analysis of 5-methyl cytosine. Image collected and cropped by CiteAb from ...read more
Immunocytochemistry/ Immunofluorescence: 5-MethylCytosine Antibody [NB100-744] - Positive staining of rat neuron cells performed with 2% BSA/0.1% Tween 20 in PBS blocking and incubated at 4 degrees C overnight using ...read more
Biological Strategies: Western Blot: 5-MethylCytosine Antibody [NB100-744] - Immunoblotting analysis shows decrease in 5-methyl cytosine and increase in H3K9 acetylation levels after combined treatment. Lanes 1 ...read more
This is specific for 5-Methyl Cytosine. It was tested using immunoprecipitation against 5-Methyl Cytosine and bromodeoxyuridine (BrdU) or a control (no antigen).
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Human reactivity reported in scientific literature (PMID: 20808872).
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.5)
Preservative
No Preservative
Concentration
3.0 mg/ml
Purity
Protein G purified
Alternate Names for 5-MethylCytosine Antibody
5-mC
5-Methyl Cytosine
Cytosine (5-Methyl)
Background
5-Methylcytosine is a methylated form of the DNA base cytosine. When cytosine is methylated, the DNA maintains the same sequence, but genes can be turned on and off (the study of this is called epigenetics).This methyl group distinguishes 5-methylcytosine from cytosine.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for 5-MethylCytosine Antibody (NB100-744)(4)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 3 applications: IF/IHC, IP, WB.
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Product General Protocols
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![Western Blot: 5-MethylCytosine Antibody [NB100-744] - Global epigenetic changes in HFFs 7 days after hESC extract treatment. (B) Immunoblotting analysis of 5-methyl cytosine. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0006.jpg "Western Blot: 5-MethylCytosine Antibody [NB100-744] - Global epigenetic changes in HFFs 7 days after hESC extract treatment. (B) Immunoblotting analysis of 5-methyl cytosine. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody [NB100-744] - Positive staining of rat neuron cells performed with 2% BSA/0.1% Tween 20 in PBS blocking and incubated at 4 degrees C overnight using NB100-744 at a dilution of 1:200.](http://images.novusbio.com/fullsize/Cytosine-5-Methyl-Antibody-Immunocytochemistry-Immunofluorescence-NB100-744-img0004.jpg "Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody [NB100-744] - Positive staining of rat neuron cells performed with 2% BSA/0.1% Tween 20 in PBS blocking and incubated at 4 degrees C overnight using NB100-744 at a dilution of 1:200.")
![Western Blot: 5-MethylCytosine Antibody [NB100-744] - Immunoblotting analysis shows decrease in 5-methyl cytosine and increase in H3K9 acetylation levels after combined treatment. Lanes 1 to 5 were loaded with proteins (2 ug) from HFFs, hESCs, inhibitor treated HFFs and combined treated HFFs. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0005.jpg "Western Blot: 5-MethylCytosine Antibody [NB100-744] - Immunoblotting analysis shows decrease in 5-methyl cytosine and increase in H3K9 acetylation levels after combined treatment. Lanes 1 to 5 were loaded with proteins (2 ug) from HFFs, hESCs, inhibitor treated HFFs and combined treated HFFs. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.")
