Choose from nearly 1,000 secondary antibodies conjugated to more than 40 different enzymatic, fluorescent, or biotin labels. Use the filters on the left to sort our secondary antibodies by species, application, host, or conjugate.
To learn more about secondary antibodies, download and read our Secondary Antibody Handbook for technical information and tips to guide you when choosing a secondary antibody for your experiment.
To understand more about direct vs. indirect detection, including benefits of a secondary antibody and when to use one, please read our resources on Direct vs. Indirect Detection Methods.
![Western Blot: Mouse anti-Human IgG (H+L) Secondary Antibody (4D2D9G8) [NBP1-51523] - Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb (lane 3, 4) against human serum (lane 1, 3) and plasma (lane 2, 4).](http://images.novusbio.com/fullsize/Human-IgG-Antibody-4D2D9G8-Western-Blot-NBP1-51523-img0002.jpg "Western Blot: Mouse anti-Human IgG (H+L) Secondary Antibody (4D2D9G8) [NBP1-51523] - Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb (lane 3, 4) against human serum (lane 1, 3) and plasma (lane 2, 4).")
![Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680] [NBP3-11667] - Lane 1: Rabbit IgG, Non-denatured. Lane 2: Rabbit IgG, Denatured. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary antibody (eB182) [DyLight 680] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted: 160 kDa for non-denatured; observed: 170-180 kDa for non-denatured. Band migrates at slightly higher molecular weight.](http://images.novusbio.com/fullsize/Mouse-Pure-Blot-anti-Rabbit-IgG-H+L-Secondary-Antibody-eB182-DyLight-680-Western-Blot-NBP3-11667-img0002.jpg "Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680] [NBP3-11667] - Lane 1: Rabbit IgG, Non-denatured. Lane 2: Rabbit IgG, Denatured. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary antibody (eB182) [DyLight 680] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted: 160 kDa for non-denatured; observed: 170-180 kDa for non-denatured. Band migrates at slightly higher molecular weight.")
![Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680] [NBP3-11667] - Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary antibody (eB182) [DyLight 680].](http://images.novusbio.com/fullsize/Mouse-Pure-Blot-anti-Rabbit-IgG-H+L-Secondary-Antibody-eB182-DyLight-680-Western-Blot-NBP3-11667-img0003.jpg)
![Western Blot of Fluorescent TrueBlot(R): Anti-Rabbit IgG DyLight 680 Conjugated. Lane 1: Rabbit IgG, Non-denatured. Lane 2: Rabbit IgG, Denatured. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680]antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted: 160 kDa for non-denatured; observed: 170-180 kDa for non-denatured. Band migrates at slightly higher molecular weight.](http://images.novusbio.com/fullsize/nbp3-11667_mouse-monoclonal-mouse-pure-blot-anti-rabbit-igg-h-l-secondary-antibody-eb182-dylight-680-245202310564640.jpg)
![Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680] / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Rabbit TrueBlot(R): Anti-Rabbit IgG HRP](http://images.novusbio.com/fullsize/nbp3-11667_mouse-monoclonal-mouse-pure-blot-anti-rabbit-igg-h-l-secondary-antibody-eb182-dylight-680-255202313135150.jpg)
![Western Blot: Mouse anti-Human IgG (H+L) Secondary Antibody [NBP1-72758] - Lane 1: Human IgG. Load: 50 ng per lane. Primary antibody: Human IgG Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1:40,000 for 30 minutes at RT. Block: incubated with blocking buffer for 30 minutes at RT. Predicted/Observed size: 55 kDa, 25 kDa for Human IgG.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-72758-img0004.jpg "Western Blot: Mouse anti-Human IgG (H+L) Secondary Antibody [NBP1-72758] - Lane 1: Human IgG. Load: 50 ng per lane. Primary antibody: Human IgG Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1:40,000 for 30 minutes at RT. Block: incubated with blocking buffer for 30 minutes at RT. Predicted/Observed size: 55 kDa, 25 kDa for Human IgG.")
![Dot Blot: Mouse anti-Human IgG (H+L) Secondary Antibody [NBP1-72758] - Dot Blot: Mouse anti-Human IgG (H+L) Secondary Antibody [Rhodamine] [NBP1-73609] - Human IgG. Load: 3-fold serial dilution starting at 200 ng.close](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-H+L-Secondary-Antibody-Dot-Blot-NBP1-72758-img0005.jpg)
![Dot Blot: Mouse anti-Human IgG (H+L) Secondary Antibody [NBP1-72758] - Dot Blot results of Goat Anti-Human IgG Antibody Rhodamine Conjugate. Dots are Human IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Anti-Human IgG Antibody TRITC at 1ug/mL in blocking buffer 1hr RT. Imaged with BioRad ChemiDoc, Rhodamine filter. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-H+L-Secondary-Antibody-Dot-Blot-NBP1-72758-img0006.jpg)
![Western Blot: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Fig. 1. Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-human IgG Fab fragment (4A11). Lane 1: Normal human immunoglobulins Lane 2: Normal bovine immunoglobulins.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Secondary-Antibody-4A11-Western-Blot-NB500-467-img0001.jpg "Western Blot: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Fig. 1. Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-human IgG Fab fragment (4A11). Lane 1: Normal human immunoglobulins Lane 2: Normal bovine immunoglobulins.")
![Flow Cytometry: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) PE-CyTM7.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Secondary-Antibody-4A11-Flow-Cytometry-NB500-467-img0004.jpg)
![Flow Cytometry: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) PE.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Secondary-Antibody-4A11-Flow-Cytometry-NB500-467-img0002.jpg)
![Flow Cytometry: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) purified / GAM-APC.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Secondary-Antibody-4A11-Flow-Cytometry-NB500-467-img0003.jpg)
![Western Blot: Mouse anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72764] - Western Blot of Mouse anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Mouse anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Mouse IgG (RABBIT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Mouse-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72764-img0006.jpg "Western Blot: Mouse anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72764] - Western Blot of Mouse anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Mouse anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Mouse IgG (RABBIT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Dot Blot: Mouse anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72764] - Rabbit IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Mouse-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-72764-img0004.jpg)
![Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (4A10) [NBP1-51564] - Western blot analysis using human IgG (Fc specific) mouse mAb against different fusion proteins with human IgG(Fc specific) tag.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Fc-fragment-Secondary-Antibody-4A10-Western-Blot-NBP1-51564-img0006.jpg "Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (4A10) [NBP1-51564] - Western blot analysis using human IgG (Fc specific) mouse mAb against different fusion proteins with human IgG(Fc specific) tag.")
![Immunocytochemistry/Immunofluorescence: Mouse anti-Human IgG Fc Secondary Antibody (4A10) [NBP1-51564] - Confocal immunofluorescence analysis of HEK293 cells transfected with recombinant plasmid with human IgG Fc tag using human IgGFc mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Fc-fragment-Secondary-Antibody-4A10-Immunocytochemistry-Immunofluorescence-NBP1-51564-img0005.jpg)
![Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - IgG kappa light chain (1), IgG lambda light chain (2) and IgG Fc (3) purified from human serum were analysed by Western blotting with an antibody against IgG kappa light chain (A) and Mouse anti-Human IgG Fc Secondary antibody (EM-07)antibody against IgG Fc fragment (B).](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Fc-Secondary-Antibody-EM-07-Western-Blot-NBP1-45063-img0003.jpg "Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - IgG kappa light chain (1), IgG lambda light chain (2) and IgG Fc (3) purified from human serum were analysed by Western blotting with an antibody against IgG kappa light chain (A) and Mouse anti-Human IgG Fc Secondary antibody (EM-07)antibody against IgG Fc fragment (B).")
![ELISA: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - Detection of IgG in human serum using HRP conjugated antibody against IgG Fc fragment.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Fc-Secondary-Antibody-EM-07-ELISA-NBP1-45063-img0004.jpg)
![Flow Cytometry: Mouse anti-Human IgM Fc Secondary Antibody (CH2) [NB500-468] - Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/Human-IgM-Fc-fragment-Antibody-CH2-Flow-Cytometry-NB500-468-img0001.jpg "Flow Cytometry: Mouse anti-Human IgM Fc Secondary Antibody (CH2) [NB500-468] - Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. Cells in the lymphocyte gate were used for analysis.")
![Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (3D3cc) [NB110-8347] - Image from verified customer review using the HRP-conjugated version of mouse anti-human IgG Fc secondary antibody (3D3cc).](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Fc-fragment-Secondary-Antibody-3D3cc-Western-Blot-NB110-8347-img0002.jpg "Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (3D3cc) [NB110-8347] - Image from verified customer review using the HRP-conjugated version of mouse anti-human IgG Fc secondary antibody (3D3cc).")