![Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - IgG kappa light chain (1), IgG lambda light chain (2) and IgG Fc (3) purified from human serum were analysed by Western blotting with an antibody against IgG kappa light chain (A) and Mouse anti-Human IgG Fc Secondary antibody (EM-07)antibody against IgG Fc fragment (B).](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Fc-Secondary-Antibody-EM-07-Western-Blot-NBP1-45063-img0003.jpg "Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - IgG kappa light chain (1), IgG lambda light chain (2) and IgG Fc (3) purified from human serum were analysed by Western blotting with an antibody against IgG kappa light chain (A) and Mouse anti-Human IgG Fc Secondary antibody (EM-07)antibody against IgG Fc fragment (B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | EM-07 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Fusion protein of human IgG Fc fragment. |
| Specificity | This secondary antibody reacts with Fc part of human IgG heavy chain and with isolated Fc fragments. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Dilutions |
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| Application Notes | Western Blot and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB. It does not crossreact with IgM. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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![ELISA: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - Detection of IgG in human serum using HRP conjugated antibody against IgG Fc fragment.](http://images.novusbio.com/fullsize/Mouse-anti-Human-IgG-Fc-Secondary-Antibody-EM-07-ELISA-NBP1-45063-img0004.jpg "ELISA: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - Detection of IgG in human serum using HRP conjugated antibody against IgG Fc fragment.")