XBP1 Antibodies
We offer XBP1 Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Flow (Intracellular), Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Peptide ELISA, Sandwich ELISA, Western Blot. Each XBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our XBP1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the XBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our XBP1 polyclonal antibodies and browse our XBP1 monoclonal antibody catalog.
Alternate Names for XBP1 Antibodies
anti-XBP1 antibody, anti-TREB5 antibody, anti-X-box binding protein 1 antibody, anti-X-box-binding protein 1 antibody, anti-XBP-1 antibody, anti-XBP2Tax-responsive element-binding protein 5 antibody
9 Results for "xbp1" in Primary Antibodies
![Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77681-img0006.jpg "Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77681-img0010.jpg)
![Immunohistochemistry: XBP1 Antibody [NBP1-77681] - Staining of XBP1 in mouse bladder using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-NBP1-77681-img0004.jpg)
![Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on NIH3T3 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/XBP1-Antibody-Flow-Cytometry-NBP1-77681-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77681-img0009.jpg)
![Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on HepG2 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/XBP1-Antibody-Flow-Cytometry-NBP1-77681-img0007.jpg)
![Western Blot: XBP1 Antibody [NBP1-77681] - Western Blot of PC-3 cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-77681_rabbit-polyclonal-xbp1-antibody-western-blot-672023151929..jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of lysates per lane. Antibodies: XBP-1 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution. (1) the absence and (2) the p](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0021.jpg "Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of lysates per lane. Antibodies: XBP-1 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution. (1) the absence and (2) the p")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77253] - analysis of 4% paraformaldehydefixed human pancreas tissue labeling XBP-1 with this antibody at20 ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77253-img0017.jpg)
![Immunohistochemistry-Paraffin: XBP1 Antibody [NBP1-77253] - analysis of paraffin-embeddedmouse spleen tissue using anti-XBP-1 antibody at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-77253-img0013.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Western Blot Validation in Human HepG2 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: XBP-1 NBP1-77253 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0009.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - ob/+ and ob/ob mice were injected with an adenovirus encoding human GRP78 (Ad GRP78) and a control adenovirus encoding beta-gal (Ad beta-gal). Mature nuclear form of XBP-1 expression level was reduced in ob/ob mice with Ad GRP78 as compared to the control Ad beta-gal while there was no difference in ob/+ mice.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0010.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of HepG2 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane1: 1 ug/mL Lane2: 2 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0018.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of A549 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goatanti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane1:0.25 ug/mL Lane2: 0.5 ug/mL Lane3: 1 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0019.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 100 ng of human XBP-1 recombinant protein perlane. Antibodies: XBP-1 (A: 0.5 ug/mL, B: 1 ug/mL andC: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77253] - analysis of 4% paraformaldehydefixed Human Liver Tissue labeling XBP-1 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (green) and DAPI staining (blue](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77253-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77253] - analysis of 4% paraformaldehydefixed human pancreas tissue labeling XBP-1 with this antibody at20 ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77253-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77253] - analysis of 4% paraformaldehydefixed HepG2 cells labeling XBP-1 with this antibody at 20 ug/mL,followed by goat anti-rabbit IgG secondary antibody at1/500 dilution (green) and DAPI staining (blue)](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77253-img0016.jpg)
![Immunohistochemistry-Paraffin: XBP1 Antibody [NBP1-77253] - Analysis of paraffin-embedded RatLiver Tissue using anti-XBP-1 antibody at 5 ug/ml.Tissue was fixed with formaldehyde and blocked with 10%serum for 1 h at RT; antigen retrieval was by heatmediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-77253-img0011.jpg)
![Immunohistochemistry-Paraffin: XBP1 Antibody [NBP1-77253] - Analysis of paraffin-embeddedmouse testis tissue using anti-XBP-1 antibody at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-77253-img0012.jpg)
![Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0004.jpg "Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77252] - HepG2 cells with XBP-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77252-img0002.jpg)
![Western Blot: XBP1 Antibody [NBP1-77252] - PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0003.jpg)
![Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0002.jpg "Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Western Blot: XBP1 Antibody [NBP2-20917] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0001.jpg)
![Western Blot: XBP1 Antibody (3H1G4) [NBP1-75514] - analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/XBP1-Antibody-3H1G4-Western-Blot-NBP1-75514-img0001.jpg "Western Blot: XBP1 Antibody (3H1G4) [NBP1-75514] - analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody (3H1G4) [NBP1-75514] - XBP-1 in HepG2 cells with XBP-1 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/XBP1-Antibody-3H1G4-Immunocytochemistry-NBP1-75514-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody (3H1G4) [NBP1-75514] - XBP1 in HepG2 cells with XBP1 antibody at 4 ug/mL.](http://images.novusbio.com/fullsize/XBP1-Antibody-3H1G4-Immunofluorescence-NBP1-75514-img0003.jpg)
![Western Blot: XBP1 Antibody (S04-3E2) [NBP3-19927] - Western blot detection of XBP1 in HL-60, U2OS cell lysates using NBP3-19927 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.](http://images.novusbio.com/fullsize/XBP1-Antibody-S04-3E2-Western-Blot-NBP3-19927-img0001.jpg "Western Blot: XBP1 Antibody (S04-3E2) [NBP3-19927] - Western blot detection of XBP1 in HL-60, U2OS cell lysates using NBP3-19927 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.")