Ubiquitin-activating Enzyme/UBE1 Antibodies
We offer Ubiquitin-activating Enzyme/UBE1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Ubiquitin-activating Enzyme/UBE1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Ubiquitin-activating Enzyme/UBE1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Ubiquitin-activating Enzyme/UBE1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Ubiquitin-activating Enzyme/UBE1 polyclonal antibodies and browse our Ubiquitin-activating Enzyme/UBE1 monoclonal antibody catalog.
Alternate Names for Ubiquitin-activating Enzyme/UBE1 Antibodies
anti-Ubiquitin-activating Enzyme/UBE1 antibody, anti-UBA1 antibody, anti-A1S9T and BN75 temperature sensitivity complementing antibody, anti-A1S9T antibody, anti-A1ST antibody, anti-AMCX1 antibody, anti-GXP1 antibody, anti-MGC4781 antibody, anti-POC20 antibody, anti-POC20 centriolar protein homolog antibody, anti-Protein A1S9 antibody, anti-SMAX2 antibody, anti-UBA1, ubiquitin-activating enzyme E1 homolog A antibody, anti-UBA1A antibody, anti-UBE1A1S9 antibody, anti-UBE1X antibody, anti-ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing) antibody, anti-Ubiquitin-activating enzyme E1 antibody, anti-ubiquitin-like modifier activating enzyme 1 antibody, anti-ubiquitin-like modifier-activating enzyme 1 antibody
9 Results for "ubiquitin-activating-enzyme-ube1" in Primary Antibodies
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Western blot analysis of extracts of various cell lines, using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-6H2I9-Western-Blot-NBP3-16792-img0003.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Western blot analysis of extracts of various cell lines, using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Immunofluorescence analysis of U-2 OS cells using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-6H2I9-Immunocytochemistry-Immunofluorescence-NBP3-16792-img0002.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Immunohistochemistry of paraffin-embedded human esophageal using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-6H2I9-Immunohistochemistry-Paraffin-NBP3-16792-img0001.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB600-472] - Shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel).](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Western-Blot-NB600-472-img0004.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB600-472] - Shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel).")
![Immunohistochemistry: Ubiquitin-activating Enzyme/UBE1 Antibody [NB600-472] - Used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Immunohistochemistry-NB600-472-img0006.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500] - Analysis using Anti-UBA1 antibody NBP2-76500 (A) shows similar pattern to independent antibody NBP1-90307 (B).](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Western-Blot-NBP2-76500-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500] - Analysis using Anti-UBA1 antibody NBP2-76500 (A) shows similar pattern to independent antibody NBP1-90307 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76500-img0002.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP1-90307] - Analysis using Anti-UBA1 antibody NBP1-90307 (A) shows similar pattern to independent antibody NBP2-76500 (B).](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Western-Blot-NBP1-90307-img0016.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP1-90307] - Analysis using Anti-UBA1 antibody NBP1-90307 (A) shows similar pattern to independent antibody NBP2-76500 (B).")
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB100-58804] - Detection of Human and Mouse UBE1 on HeLa whole cell lysate using NB100-58804. UBE1 was also IPed efficiently by rabbit anti-UBE1 antibodies NB100-58805 and NB100-58806.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Western-Blot-NB100-58804-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB100-58804] - Detection of Human and Mouse UBE1 on HeLa whole cell lysate using NB100-58804. UBE1 was also IPed efficiently by rabbit anti-UBE1 antibodies NB100-58805 and NB100-58806.")
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (2B6) [H00007317-M02] - UBA1 monoclonal antibody (M02), clone 2B6. Analysis of UBA1 expression in HeLa.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-2B6-Western-Blot-H00007317-M02-img0002.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (2B6) [H00007317-M02] - UBA1 monoclonal antibody (M02), clone 2B6. Analysis of UBA1 expression in HeLa.")
![Sandwich ELISA: Ubiquitin-activating Enzyme/UBE1 Antibody (2B6) [H00007317-M02] - Detection limit for recombinant GST tagged UBA1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-2B6-Sandwich-ELISA-H00007317-M02-img0001.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-20779] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ubiquitin-activating Enzyme/UBE1 antibody [N1N3] (NBP2-20779) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20779_rabbit-polyclonal-ubiquitin-activating-enzyme-ube1-antibody-91020242021911.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-20779] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ubiquitin-activating Enzyme/UBE1 antibody [N1N3] (NBP2-20779) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:111 kDaObserved band size:100 kDa](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-05-Western-Blot-NBP3-05789-img0001.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:111 kDaObserved band size:100 kDa")
![Immunoprecipitation: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate0.5 uL anti-UBA1 mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-UBA1 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-05-Immunoprecipitation-NBP3-05789-img0002.jpg)