TRAP1 Antibodies
We offer TRAP1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each TRAP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TRAP1 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Primate, Rat. Use the list below to choose the TRAP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TRAP1 polyclonal antibodies and browse our TRAP1 monoclonal antibody catalog.
Alternate Names for TRAP1 Antibodies
anti-TRAP1 antibody, anti-HSP 75 antibody, anti-HSP75TNFR-associated protein 1 antibody, anti-HSP90Lheat shock protein 75 kDa, mitochondrial antibody, anti-TNF receptor-associated protein 1 antibody, anti-TRAP-1 antibody, anti-tumor necrosis factor type 1 receptor associated protein antibody, anti-Tumor necrosis factor type 1 receptor-associated protein antibody
12 Results for "trap1" in Primary Antibodies
![Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Western-Blot-NB300-555-img0005.jpg "Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.")
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Analysis of TRAP1 in NCI-H460 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TRAP1 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. TRAP1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0006.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (TRAP1-6) [NB300-555] - Normal biopsies of deparaffinized Human liver tissue, dilution of 1:20.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunohistochemistry-Paraffin-NB300-555-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Immunolocalization of TRAP1 in PC-3-M cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0003.jpg)
![Immunohistochemistry: TRAP1 Antibody (TRAP1-6) [NB300-555] - Representative images of IHF staining of EP2 or EP4 (green) and TRAP1 (NB300-555) (red) in subchondral bone 2 weeks post-sham or ACLT surgery (left) and quantitative analysis (right). The white arrows indicate TRAP-positive osteoclasts expressing EP2 or EP4. Scale bars, 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-25520231539518.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - PGE2 regulates migration & osteoclast differentiation through the G alpha s/PI3K/AKT/MAPK signaling pathways downstream of EP4. PGE2 regulates migration & osteoclast differentiation independent of beta -arrestin1 (a) & beta -arrestin2 (b) but via G alpha s (c). BMMs from the WT & littermate beta -arrestin1- & beta -arrestin2-knockout mice were used to generate osteoclasts by stimulation with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL & incubation with 100 nmol·L−1 PGE2. For G alpha s experiments, osteoclasts were generated from the GMMs of the G alpha sfl/fl controls & G alpha sfl/fl; LysM-cre (G alpha sLysM) mice. A differentiation assay (TRAP staining) (left) & the corresponding quantitative analysis (right). Scale bars, 50 μm. Error bars are the mean ± s.d. n = 3. Two-way ANOVA followed by Tukey’s t tests. Scale bars, 50 μm. d cAMP production was measured via ELISAs for osteoclasts derived from the Ep4fl/fl & Ep4LysM mice. BMMs from the two mouse strains were isolated & stimulated with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL to differentiate into osteoclasts & incubated with 100 nmol·L−1 PGE2 for 30 min prior to cAMP measurements. Error bars are the mean ± s.d. ***P < 0.001 by unpaired two-tailed Student’s t test. The experiment was performed with three biological replicates. e Representative images of the indicated protein expression by western blotting for osteoclasts generated using BMMs of Ep4fl/fl & Ep4LysM mice. The cells were treated either with osteoclastogenic media (10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL) alone, with PGE2 (100 nmol·L−1), or PGE2 with IBMX (1 mmol·L−1) for 3 h. The experiments were performed with three biological replicates Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-310202416212330.jpg)
![Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP1 monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Western-Blot-NBP2-01707-img0008.jpg "Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP1.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunocytochemistry-Immunofluorescence-NBP2-01707-img0007.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Human Kidney tissue using anti-TRAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunohistochemistry-Paraffin-NBP2-01707-img0006.jpg)
![Flow Cytometry: TRAP1 Antibody (1H8) [NBP2-01707] - Analysis of Jurkat cells, using anti-TRAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Flow-Cytometry-NBP2-01707-img0001.jpg)
![Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP1.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Western-Blot-NBP2-01707-img0002.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunohistochemistry-Paraffin-NBP2-01707-img0003.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunohistochemistry-Paraffin-NBP2-01707-img0004.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1H8-Immunohistochemistry-Paraffin-NBP2-01707-img0005.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47597] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-TRAP1 antibody NBP2-47597 (A) shows similar protein distribution across tissues to independent antibody NBP2-47598 (B).](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47597-img0005.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47597] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-TRAP1 antibody NBP2-47597 (A) shows similar protein distribution across tissues to independent antibody NBP2-47598 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: TRAP1 Antibody [NBP2-47597] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47597-img0004.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47597] - Staining of human kidney shows strong to very strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47597-img0010.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47597] - Staining of human cerebellum shows moderate to strong granular cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47597-img0007.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47597] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47597-img0008.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47597] - Staining of human fallopian tube shows strong to very strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47597-img0009.jpg)
![Western Blot: TRAP1 Antibody [NBP2-47597] - Analysis in human cell line CACO-2.](http://images.novusbio.com/fullsize/antibody/nbp2-47597_rabbit-polyclonal-trap1-antibody-western-blot-12320248571..jpg)
![Western Blot: TRAP1 Antibody [NBP2-47598] - Analysis using Anti-TRAP1 antibody NBP2-47598 (A) shows similar pattern to independent antibody NBP2-47597 (B).](http://images.novusbio.com/fullsize/TRAP1-Antibody-Western-Blot-NBP2-47598-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TRAP1 Antibody [NBP2-47598] - Analysis using Anti-TRAP1 antibody NBP2-47598 (A) shows similar pattern to independent antibody NBP2-47597 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: TRAP1 Antibody [NBP2-47598] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47598-img0004.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47598] - Staining of human fallopian tube shows strong to very strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47598-img0008.jpg)
![Immunohistochemistry: TRAP1 Antibody [NBP2-47598] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules. Moderate staining was observed in cells in glomeruli.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-NBP2-47598-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47598] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-TRAP1 antibody NBP2-47598 (A) shows similar protein distribution across tissues to independent antibody NBP2-47597 (B).](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47598-img0005.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47598] - Staining of human cerebellum shows moderate to strong granular cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47598-img0007.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-47598] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-47598-img0009.jpg)
![Western Blot: TRAP1 Antibody [NBP1-32097] - Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Western-Blot-NBP1-32097-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TRAP1 Antibody [NBP1-32097] - Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TRAP1 Antibody [NBP1-32097] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: TRAP1 protein stained by TRAP1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32097-img0005.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP1-32097] - Paraffin-embedded mouse intestine. TRAP1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-32097-img0006.jpg)
![Western Blot: TRAP1 Antibody [NBP1-32097] - Sample(30 ug of whole cell lysate)A:Hep G2 B:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:1500.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Western-Blot-NBP1-32097-img0004.jpg)
![Western Blot: TRAP1 Antibody [NBP1-32097] -Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32097_rabbit-polyclonal-trap1-antibody-western-blot-30720249479..jpg)
![Western Blot: TRAP1 Antibody [NBP2-20700] - Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Western-Blot-NBP2-20700-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TRAP1 Antibody [NBP2-20700] - Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TRAP1 Antibody [NBP2-20700] - HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and permeabilized with 100% MeOH for 30 sec. Green: TRAP1 protein stained by TRAP1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20700-img0003.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-20700] - Ovarian carcinoma. TRAP1 antibody [N1N3] dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20700-img0002.jpg)
![Western Blot: TRAP1 Antibody [NBP2-20700] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Western-Blot-NBP2-20700-img0001.jpg)
![Western Blot: TRAP1 Antibody (3H4-2H6) [NBP2-59343] - Analysis of Human, Rat Human A431 and Rat Brain Membrane cell lysates showing detection of ~75 kDa Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6. Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Rat lysate, Rat Brain Membrane (RBM). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Color Development: TMB solution for 15 min at RT. Predicted/Observed Size: ~75 kDa.](http://images.novusbio.com/fullsize/TRAP1-Antibody-3H4-2H6-Western-Blot-NBP2-59343-img0004.jpg "Western Blot: TRAP1 Antibody (3H4-2H6) [NBP2-59343] - Analysis of Human, Rat Human A431 and Rat Brain Membrane cell lysates showing detection of ~75 kDa Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6. Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Rat lysate, Rat Brain Membrane (RBM). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Color Development: TMB solution for 15 min at RT. Predicted/Observed Size: ~75 kDa.")
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (3H4-2H6) [NBP2-59343] - Tissue: Myoblast cell line C2C12 . Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/TRAP1-Antibody-3H4-2H6-Immunocytochemistry-Immunofluorescence-NBP2-59343-img0002.jpg)
![Immunohistochemistry;TRAP1 Antibody[NBP3-29104]Detection of human TRAP1/HSP75 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TRAP1/HSP75 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29105_rabbit-trap1-pab-7820241034358.jpg "Immunohistochemistry;TRAP1 Antibody[NBP3-29104]Detection of human TRAP1/HSP75 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TRAP1/HSP75 used at a dilution of 1:1,000 . Detection: DAB")
![Immunoprecipitation:TRAP1 AntibodyNBP3-29105] -Detection of human TRAP1/HSP75 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAP1/HSP75 antibody NBP3-29105 (lot NBP3-29105-1) used for IP at 6 µg per reaction. TRAP1/HSP75 was also immunoprecipitated by rabbit anti-TRAP1/HSP75.. For blotting immunoprecipitated TRAP1/HSP75, NBP3-29105 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29105_rabbit-trap1-pab-301220241556514.jpg)
![Immunohistochemistry;TRAP1 Antibody[NBP3-30427]Detection of human TRAP1/HSP75 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TRAP1/HSP75 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30428_rabbit-trap1-pab-78202410343517.jpg "Immunohistochemistry;TRAP1 Antibody[NBP3-30427]Detection of human TRAP1/HSP75 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TRAP1/HSP75 used at a dilution of 1:1,000 . Detection: DAB")
![Immunoprecipitation:TRAP1 AntibodyNBP3-30428] -Detection of human TRAP1/HSP75 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAP1/HSP75 antibody NBP3-30428 (lot NBP3-30428-1) used for IP at 6 µg per reaction. TRAP1/HSP75 was also immunoprecipitated by rabbit anti-TRAP1/HSP75.. For blotting immunoprecipitated TRAP1/HSP75, NBP3-30428 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30428_rabbit-trap1-pab-301220241620455.jpg)
![Western Blot: TRAP1 Antibody (1L8C1) [NBP3-16303] - Western blot analysis of extracts of various cell lines, using TRAP1 Rabbit mAb (NBP3-16303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1L8C1-Western-Blot-NBP3-16303-img0001.jpg "Western Blot: TRAP1 Antibody (1L8C1) [NBP3-16303] - Western blot analysis of extracts of various cell lines, using TRAP1 Rabbit mAb (NBP3-16303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunoprecipitation: TRAP1 Antibody (1L8C1) [NBP3-16303] - Immunoprecipitation analysis of 300ug extracts of HCT116 cells using 3ug TRAP1 antibody (NBP3-16303). Western blot was performed from the immunoprecipitate using TRAP1 antibody (NBP3-16303) at a dilition of 1:500.](http://images.novusbio.com/fullsize/TRAP1-Antibody-1L8C1-Immunoprecipitation-NBP3-16303-img0002.jpg)
![Western Blot: TRAP1 Antibody (9B6) [NBP1-97497] - Analysis of TRAP1 probed with Lane 1: MWM, Lane 2: TRAP1 Recombinant Human Protein, Lane 3: HL-60 Cell Lysate, Lane 4: Hep G2 Cell Lysate, Lane 5: 3T3 Cell Lysate, Heat Shocked, Lane 6: HeLa Cell Lysate, Heat Shocked](http://images.novusbio.com/fullsize/TRAP1-Antibody-9B6-Western-Blot-NBP1-97497-img0001.jpg "Western Blot: TRAP1 Antibody (9B6) [NBP1-97497] - Analysis of TRAP1 probed with Lane 1: MWM, Lane 2: TRAP1 Recombinant Human Protein, Lane 3: HL-60 Cell Lysate, Lane 4: Hep G2 Cell Lysate, Lane 5: 3T3 Cell Lysate, Heat Shocked, Lane 6: HeLa Cell Lysate, Heat Shocked")