TMED9 Antibodies
We offer TMED9 Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each TMED9 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TMED9 Antibodies can be used in a variety of model species: Human. Use the list below to choose the TMED9 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TMED9 polyclonal antibodies.
Alternate Names for TMED9 Antibodies
anti-TMED9 antibody, anti-Glycoprotein 25L2 antibody, anti-GP25L2 antibody, anti-gp25L2 protein antibody, anti-HSGP25L2G antibody, anti-transmembrane emp24 domain-containing protein 9 antibody, anti-transmembrane emp24 protein transport domain containing 9 antibody
8 Results for "tmed9" in Primary Antibodies
![Western Blot: TMED9 Antibody (A9A8) [NBP3-33026] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-33026) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: THP-1 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: PC-12 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 27 kDa Observed band size: 24 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33026) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33026_mouse-tmed9-mab-a9a8-288202412493439.jpg "Western Blot: TMED9 Antibody (A9A8) [NBP3-33026] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-33026) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: THP-1 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: PC-12 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 27 kDa Observed band size: 24 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33026) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TMED9 Antibody (A9A8) [NBP3-33026] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMED9 antibody (NBP3-33026) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33026) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33026_mouse-tmed9-mab-a9a8-29820241435294.jpg)
![Immunocytochemistry/ Immunofluorescence: TMED9 Antibody (A9A8) [NBP3-33026] - Immunocytochemistry analysis of HepG2 cells labeling TMED9 with Mouse anti-TMED9 antibody (NBP3-33026) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-TMED9 antibody (NBP3-33026) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33026_mouse-tmed9-mab-a9a8-2920241192351.jpg)
![Western Blot: TMED9 Antibody (A9A8-R) [NBP3-33028] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-33028) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: THP-1 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: PC-12 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 27 kDa Observed band size: 24 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33028) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33028_mouse-tmed9-mab-a9a8-r-288202412493450.jpg "Western Blot: TMED9 Antibody (A9A8-R) [NBP3-33028] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-33028) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: THP-1 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: PC-12 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 27 kDa Observed band size: 24 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33028) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TMED9 Antibody (A9A8-R) [NBP3-33028] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMED9 antibody (NBP3-33028) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33028) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33028_mouse-tmed9-mab-a9a8-r-29820241492645.jpg)
![Immunocytochemistry/ Immunofluorescence: TMED9 Antibody (A9A8-R) [NBP3-33028] - Immunocytochemistry analysis of HepG2 cells labeling TMED9 with Mouse anti-TMED9 antibody (NBP3-33028) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-TMED9 antibody (NBP3-33028) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33028_mouse-tmed9-mab-a9a8-r-2920241033407.jpg)
![Immunohistochemistry-Paraffin: TMED9 Antibody [NBP1-31650] - Mouse duodenum. TMED9 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TMED9-Antibody-Immunohistochemistry-Paraffin-NBP1-31650-img0004.jpg "Immunohistochemistry-Paraffin: TMED9 Antibody [NBP1-31650] - Mouse duodenum. TMED9 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: TMED9 Antibody [NBP1-31650] - DLD-1 xenograft, using TMED9 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TMED9-Antibody-Immunohistochemistry-Paraffin-NBP1-31650-img0002.jpg)
![Western Blot: TMED9 Antibody [NBP1-31650] - MCF-7 whole cell and membrane extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TMED9 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31650_rabbit-polyclonal-tmed9-antibody-western-blot-29520249394..jpg)
![Western Blot: TMED9 Antibody [NBP1-31650] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with TMED9 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31650_rabbit-polyclonal-tmed9-antibody-western-blot-295202494029..jpg)
![Western Blot: TMED9 Antibody [NBP1-83193] - Analysis in rat cell line NBT-II.](http://images.novusbio.com/fullsize/TMED9-Antibody-Western-Blot-NBP1-83193-img0007.jpg "Western Blot: TMED9 Antibody [NBP1-83193] - Analysis in rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: TMED9 Antibody [NBP1-83193] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/TMED9-Antibody-Immunohistochemistry-Paraffin-NBP1-83193-img0001.jpg)
![Western Blot: TMED9 Antibody [NBP1-83193] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/TMED9-Antibody-Western-Blot-NBP1-83193-img0006.jpg)
![Western Blot: TMED9 Antibody (A9A7-R) [NBP3-33027] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-33027) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: LO2 cell lysate Lane 4: THP-1 cell lysate Lane 5: A431 cell lysate Lane 6: A549 cell lysate Lane 7: MCF-7 cell lysate Lane 8: Raji cell lysate Lane 9: PC-12 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse lung tissue lysate Lane 13: Mouse liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 10 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33027) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33027_mouse-tmed9-mab-a9a7-r-288202412493442.jpg "Western Blot: TMED9 Antibody (A9A7-R) [NBP3-33027] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-33027) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: LO2 cell lysate Lane 4: THP-1 cell lysate Lane 5: A431 cell lysate Lane 6: A549 cell lysate Lane 7: MCF-7 cell lysate Lane 8: Raji cell lysate Lane 9: PC-12 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse lung tissue lysate Lane 13: Mouse liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 10 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33027) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TMED9 Antibody (A9A7-R) [NBP3-33027] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMED9 antibody (NBP3-33027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33027_mouse-tmed9-mab-a9a7-r-298202414262667.jpg)
![Western Blot: TMED9 Antibody (A9A7) [NBP3-32938] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-32938) at 1/5,000 dilution. Lane 1: Hela cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (10 ug/Lane) Lane 3: A549 cell lysate (10 ug/Lane) Lane 4: MCF-7 cell lysate (10 ug/Lane) Lane 5: PC-12 cell lysate (10 ug/Lane) Lane 6: NIH/3T3 cell lysate (10 ug/Lane) Lane 7: Mouse liver tissue lysate (20 ug/Lane) Lane 8: Mouse lung tissue lysate (20 ug/Lane) Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32938) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32938_mouse-tmed9-mab-a9a7-288202412442934.jpg "Western Blot: TMED9 Antibody (A9A7) [NBP3-32938] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-32938) at 1/5,000 dilution. Lane 1: Hela cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (10 ug/Lane) Lane 3: A549 cell lysate (10 ug/Lane) Lane 4: MCF-7 cell lysate (10 ug/Lane) Lane 5: PC-12 cell lysate (10 ug/Lane) Lane 6: NIH/3T3 cell lysate (10 ug/Lane) Lane 7: Mouse liver tissue lysate (20 ug/Lane) Lane 8: Mouse lung tissue lysate (20 ug/Lane) Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32938) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TMED9 Antibody (A9A7) [NBP3-32938] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-TMED9 antibody (NBP3-32938) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32938) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32938_mouse-tmed9-mab-a9a7-298202414161860.jpg)
![Western Blot: TMED9 Antibody [NBP2-94854] - Analysis of extracts of various cell lines, using TMED9 at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/TMED9-Antibody-Western-Blot-NBP2-94854-img0001.jpg "Western Blot: TMED9 Antibody [NBP2-94854] - Analysis of extracts of various cell lines, using TMED9 at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Western Blot: TMED9 Antibody [NBP2-85935] - Host: Rabbit. Target Name: TMED9. Sample Tissue: Human THP-1 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/TMED9-Antibody-Western-Blot-NBP2-85935-img0001.jpg "Western Blot: TMED9 Antibody [NBP2-85935] - Host: Rabbit. Target Name: TMED9. Sample Tissue: Human THP-1 Whole Cell lysates. Antibody Dilution: 1ug/ml")