TFEB Antibodies
We offer TFEB Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each TFEB Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TFEB Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the TFEB Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TFEB polyclonal antibodies and browse our TFEB monoclonal antibody catalog.
Alternate Names for TFEB Antibodies
anti-TFEB antibody, anti-ALPHATFEB antibody, anti-BHLHE35 antibody, anti-Class E basic helix-loop-helix protein 35 antibody, anti-T-cell transcription factor EB antibody, anti-TCFEBbHLHe35 antibody, anti-transcription factor EB antibody
13 Results for "tfeb" in Primary Antibodies
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB monoclonal antibody (M01), clone S1. Analysis of TFEB expression in rat brain.](http://images.novusbio.com/fullsize/TFEB-Antibody-S1-Western-Blot-H00007942-M01-img0009.jpg "Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB monoclonal antibody (M01), clone S1. Analysis of TFEB expression in rat brain.")
![Immunocytochemistry/Immunofluorescence: TFEB Antibody (S1) [H00007942-M01] - Analysis of monoclonal antibody to TFEB on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/TFEB-Antibody-S1-Immunocytochemistry-Immunofluorescence-H00007942-M01-img0010.jpg)
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB monoclonal antibody (M01), clone 3E1-G6 Western Blot analysis of TFEB expression in Jurkat ( Cat # L017V1 ).](http://images.novusbio.com/fullsize/TFEB-Antibody-S1-Western-Blot-H00007942-M01-img0011.jpg)
![ELISA: TFEB Antibody (S1) [H00007942-M01] - TFEB Antibody (3E1-G6) [H00007942-M01] - Detection limit for recombinant GST tagged TFEB is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TFEB-Antibody-S1-ELISA-H00007942-M01-img0008.jpg)
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - GDF11 inhibits TFEB activity. (a) AML‐12 cells were cultured for 48 h in presence of GDF11 (100 ng/ml). qRT‐PCR analysis of the mRNA levels of TFEB & its target genes VPS11 & ATP6V1H. (b) AML‐12 cells transient expressing GFP‐TFEB were cultured for 48 h in presence of GDF11 (100 ng/ml). Immunofluorescence confocal microscopy showing TFEB localization with or without GDF11 (original magnification, 400×). (c) Nuclear TFEB fluorescence intensity normalized to vehicle controls. (d) Western blot analysis of total TFEB protein expression. (e) Densitometry analysis of the total TFEB. (f) Western blot analysis of nuclear TFEB protein expression. (g) Densitometry analysis of the nuclear TFEB. The experiment was performed in triplicate with similar results. The data are shown as mean ± SD, *p < 0.05 compared to the vehicle group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34905649), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007942-m01_mouse-monoclonal-tfeb-antibody-s1-310202415482264.jpg)
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB expression reduces PHF1 levels in the P301S model of tauopathy, & significantly attenuates the loss of neuronal & synaptic markers in the hippocampus. A, Lysates were prepared from the hippocampal regions of WT, P301S, & P301S/flag-TFEB mice, & were subjected to immunoblotting. B, Quantitation by ImageJ revealed PHF1 levels of 436% in the P301S mice compared with WT controls, which were significantly attenuated to 212% in the P301S/flag-TFEB double-transgenic mice. However, TFEB expression did not alter the levels of total tau, which remained similar in the P301S & the double-transgenic mice. NeuN levels were reduced by 25% in the P301S mice, which was significantly reversed to WT levels in the double-transgenic mice. Similarly, spinophilin levels were reversed from 40% reduction to 6% due to TFEB overexpression. TFEB expression also increased LAMP1 levels by 207% & cathepsin D levels by 33% in the P301S/flag-TFEB mice compared with WT controls. Statistical analysis by one-way ANOVA followed by Student–Newman–Keuls post hoc test revealed significant differences. *p < 0.05, **p < 0.01, & ***p < 0.001, compared with WT controls; $$p < 0.01, $$$p < 0.001, compared with P301S mice. The data are reported as the mean ± SEM. n = 4 WT mice; n = 5 P301S & P301S/flag-TFEB mice. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0042-16.2016), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007942-m01_mouse-monoclonal-tfeb-antibody-s1-310202415482238.jpg)
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - GDF11 inhibits TFEB activity. (a) AML‐12 cells were cultured for 48 h in presence of GDF11 (100 ng/ml). qRT‐PCR analysis of the mRNA levels of TFEB & its target genes VPS11 & ATP6V1H. (b) AML‐12 cells transient expressing GFP‐TFEB were cultured for 48 h in presence of GDF11 (100 ng/ml). Immunofluorescence confocal microscopy showing TFEB localization with or without GDF11 (original magnification, 400×). (c) Nuclear TFEB fluorescence intensity normalized to vehicle controls. (d) Western blot analysis of total TFEB protein expression. (e) Densitometry analysis of the total TFEB. (f) Western blot analysis of nuclear TFEB protein expression. (g) Densitometry analysis of the nuclear TFEB. The experiment was performed in triplicate with similar results. The data are shown as mean ± SD, *p < 0.05 compared to the vehicle group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34905649), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007942-m01_mouse-monoclonal-tfeb-antibody-s1-310202415483630.jpg)
![Western Blot: TFEB Antibody [NBP2-41167] - Analysis of TFEB in A549 cell lysate with TFEB antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/TFEB-Antibody-Western-Blot-NBP2-41167-img0001.jpg "Western Blot: TFEB Antibody [NBP2-41167] - Analysis of TFEB in A549 cell lysate with TFEB antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: TFEB Antibody [NBP2-41167] - Human lung tissue with TFEB antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/TFEB-Antibody-Immunofluorescence-NBP2-41167-img0003.jpg)
![Immunohistochemistry-Paraffin: TFEB Antibody [NBP2-41167] - Human lung tissue with TFEB (NT) antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/TFEB-Antibody-Immunohistochemistry-Paraffin-NBP2-41167-img0002.jpg)
![Immunohistochemistry: TFEB Antibody - BSA Free [NBP2-41167] - Immunohistochemistry of TFEB (NT) in human lung tissue with TFEB (NT) antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41167_rabbit-polyclonal-tfeb-antibody-271120242017514.jpg)
![Immunohistochemistry: TFEB Antibody - BSA Free [NBP2-41167] - Immunohistochemistry of TFEB (NT) in human lung tissue with TFEB (NT) antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41167_rabbit-polyclonal-tfeb-antibody-2711202419333484.jpg)
![Immunocytochemistry/ Immunofluorescence: TFEB Antibody - BSA Free [NBP2-41167] - Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41167_rabbit-polyclonal-tfeb-antibody-271120241928887.jpg)
![Immunocytochemistry/ Immunofluorescence: TFEB Antibody - BSA Free [NBP2-41167] - Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41167_rabbit-polyclonal-tfeb-antibody-271120242045661.jpg)
![Immunocytochemistry/Immunofluorescence: TFEB Antibody [NBP2-41168] - Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/TFEB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41168-img0003.jpg "Immunocytochemistry/Immunofluorescence: TFEB Antibody [NBP2-41168] - Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 ug/mL.")
![Immunohistochemistry-Paraffin: TFEB Antibody [NBP2-41168] - Human lung tissue with TFEB antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/TFEB-Antibody-Immunohistochemistry-NBP2-41168-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: TFEB Antibody - BSA Free [NBP2-41168] - Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41168_rabbit-polyclonal-tfeb-antibody-271120241918629.jpg)
![Immunocytochemistry/ Immunofluorescence: TFEB Antibody - BSA Free [NBP2-41168] - Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41168_rabbit-polyclonal-tfeb-antibody-271120242016988.jpg)
![Western Blot: TFEB Antibody (BLR070G) [NBP2-77360] - Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1,and BW5147.3 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-TFEB recombinant monoclonalantibody [BLR070G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/TFEB-Antibody-BLR070G-Western-Blot-NBP2-77360-img0004.jpg "Western Blot: TFEB Antibody (BLR070G) [NBP2-77360] - Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1,and BW5147.3 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-TFEB recombinant monoclonalantibody [BLR070G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].")
![Western Blot: TFEB Antibody (BLR070G) [NBP2-77360] - Whole cell lysate (50 ug) from HeLa, Hep-G2, MCF-7, Jurkat, andK-562 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-TFEB recombinant monoclonal antibody[BLR070G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 3 minutes.Lower Panel: Rabbit anti-Actin recombinant monoclonalantibody [BLR057F].](http://images.novusbio.com/fullsize/TFEB-Antibody-BLR070G-Western-Blot-NBP2-77360-img0003.jpg)
![<b>Independent Antibodies Validation. </b>TFEB Antibody (BLR070G) [NBP2-77360] - Detection of human TFEB by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-TFEB recombinant monoclonal [BLR070G] (NBP2-77360) and rabbit anti-TFEB antibodies (NBP2-12758, other rabbit anti-TFEB antibody).](http://images.novusbio.com/fullsize/TFEB-Antibody-BLR070G-NBP2-77360-img0002.jpg)
![Western Blot: TFEB Antibody [NB100-93447] - Representative western blots of Atg7, TFEB and p62 at different times after LPS injection. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-08722-3), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TFEB-Antibody-Western-Blot-NB100-93447-img0004.jpg "Western Blot: TFEB Antibody [NB100-93447] - Representative western blots of Atg7, TFEB and p62 at different times after LPS injection. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-08722-3), licensed under a CC-BY license.")
![Western Blot: TFEB Antibody [NB100-93447] - (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TFEB-Antibody-Western-Blot-NB100-93447-img0003.jpg)
![Western Blot: TFEB Antibody [NBP2-12758] - Detection of Human TFEB by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 10 second.](http://images.novusbio.com/fullsize/TFEB-Antibody-Western-Blot-NBP2-12758-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: TFEB Antibody [NBP2-12758] - Detection of Human TFEB by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 10 second.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: TFEB Antibody [NBP2-12758] - Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for IP at 6 ug per reaction. TFEB was also immunoprecipitated by a different rabbit anti-TFEB antibody and rabbit anti-TFEB recombinant monoclonal antibody (NBP2-77360). For blotting immunoprecipitated TFEB, NBP2-77360 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/TFEB-Antibody-Immunoprecipitation-NBP2-12758-img0004.jpg)
![Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10Lane 2: RT-4Lane 3: U-251MGLane 4: Human PlasmaLane 5: LiverLane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-25187_rabbit-tfeb-pab-2012202314554918.jpg)
![Western Blot: TFEB Antibody (HL1065) [NBP3-13706] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with TFEB antibody [HL1065] (NBP3-13706) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TFEB-Antibody-HL1065-Western-Blot-NBP3-13706-img0001.jpg "Western Blot: TFEB Antibody (HL1065) [NBP3-13706] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with TFEB antibody [HL1065] (NBP3-13706) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: TFEB Antibody (HL1065) [NBP3-13706] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TFEB antibody [HL1065] (NBP3-13706) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13706_rabbit-tfeb-mab-hl1065-91020242014655.jpg)
![Immunohistochemistry-Paraffin: TFEB Antibody (HL1065) [NBP3-13706] - TFEB antibody [HL1065] detects TFEB protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.TFEB stained by TFEB antibody [HL1065] (NBP3-13706) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13706_rabbit-tfeb-mab-hl1065-91020242075942.jpg)
![Western Blot: TFEB Antibody (S05-0D7) [NBP3-14901] - Western blot detection of TFEB in A549, HL-60 cell lysates using TFEB Rabbit mAb (1:1000 diluted). Predicted band size: 53kDa. Observed band size: 65-70kDa.](http://images.novusbio.com/fullsize/TFEB-Antibody-S05-0D7-Western-Blot-NBP3-14901-img0001.jpg "Western Blot: TFEB Antibody (S05-0D7) [NBP3-14901] - Western blot detection of TFEB in A549, HL-60 cell lysates using TFEB Rabbit mAb (1:1000 diluted). Predicted band size: 53kDa. Observed band size: 65-70kDa.")
![Immunohistochemistry: TFEB Antibody (S05-0D7) [NBP3-14901] - Immunohistochemical of TFEB in Human lung cancer tissue using TFEB antibody at dilution 1/20](http://images.novusbio.com/fullsize/TFEB-Antibody-S05-0D7-Immunohistochemistry-NBP3-14901-img0002.jpg)