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Spi-B Antibodies

We offer Spi-B Antibodies for use in common research applications: ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each Spi-B Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Spi-B Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Human, Mouse, Primate. Use the list below to choose the Spi-B Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Spi-B polyclonal antibodies and browse our Spi-B monoclonal antibody catalog.

Alternate Names for Spi-B Antibodies

anti-Spi-B antibody, anti-SPIB antibody, anti-Spi-B transcription factor (Spi-1/PU.1 related) antibody, anti-transcription factor Spi-B antibody

5 Results for "spi-b" in Primary Antibodies

AF7204

Spi-B Antibody

(8 Publications)

100 μg

AF7204

Spi-B was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Mouse splenocytes were stained with Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P). Quadrant markers were set based on control antibody staining (Catalog # 5‑001‑A). To facilitate intracellular staining, cells were fixed with paraformadehyde and permeabilized with saponin.

OPGhigh M cells cluster more in cecal patches than in Peyer’s patches.a Whole-mount immunostaining of the FAE of Peyer’s patches (left) and cecal patches (right) for OPG (green) and Spi-B (red). Nuclei were stained with DAPI (blue); scale bars: 50 µm. b Scatter plots of the fluorescence intensities of OPG versus Spi-B. Red dots represent cells stained with anti-Spi-B antibody that was conjugated with HyLyte Fluor 555 and anti-OPG antibody that was conjugated with HyLyte Fluor 647. Blue dots represent background fluorescence intensity of randomly selected non-stained cells. Fluorescence intensities were measured for at least 3000 cells from five FAEs of three mice. c Frequencies of OPGhigh M cells in Peyer’s patches and cecal patches were quantified. *p < 0.05, ***p < 0.005. Student’s t-test, n = 5 FAE from three animals. The source data underlying panels b and c are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932605), licensed under a CC-BY license. Not internally tested by R&D Systems.

$RANKL–RANK signaling is stimulated in the gut epithelia of Opg−/− mice.a GP2+ cells (red) are more effectively induced in the cecal epithelium (CE) and ileal villi (VE) of Opg−/− mice by RANKL administration. Whole-mount immunohistochemical images of Spi-B (green) and GP2 (red) in the VE and CE of WT (upper panels) and Opg−/− mice (lower panels) treated with either GST (control; left) or GST-RANKL (right). Scale bars: 100 µm. Representative images from three independent experiment are shown. b Quantitative PCR analysis of M-cell marker expression in conventional epithelia from the VE and CE of mice injected with GST (control) or GST-RANKL. Results were normalized to Gapdh expression and are presented relative to the expression in the ileal epithelium from GST-treated mice. Data shown are mean values from three independent experiments (error bars indicate standard deviation). ***p < 0.005, **p < 0.01, *p < 0.05; p values were calculated with the Student’s t-test (n = 3 biologically independent experiments). c, d Nuclear translocation activities of RelB and p52 following RANKL stimulation are enhanced in Opg−/− mice. c Western blot analysis of p100/p52 and RelB in the VE and CE of mice injected with GST or GST-RANKL. Rpt4, a subunit of the 26S proteasome, was used as an internal control for the cytoplasmic fraction. Lamin A/C (Lamin) was used as an internal control for the nuclear fraction. Data are representative of two independent experiments. d Right, single confocal planes of the cecal FAE from WT and Opg−/− mice. FAE monolayers were stained with anti-RelB (green) and anti-Spi-B (red) antibodies and with Hoechst 33342. Left, a bar graph summarizing the proportions of RelB-positive cells among total numbers of M cells (Spi-B-positive cells). ***p < 0.005; p values were calculated with the Student’s t-test (n = 4 biologically independent experiments). Scale bars: 20 µm. The source data underlying panels b and d and non-cropped scan images of western blotting (c) are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932605), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$RANKL–RANK signaling is stimulated in the gut epithelia of Opg−/− mice.a GP2+ cells (red) are more effectively induced in the cecal epithelium (CE) and ileal villi (VE) of Opg−/− mice by RANKL administration. Whole-mount immunohistochemical images of Spi-B (green) and GP2 (red) in the VE and CE of WT (upper panels) and Opg−/− mice (lower panels) treated with either GST (control; left) or GST-RANKL (right). Scale bars: 100 µm. Representative images from three independent experiment are shown. b Quantitative PCR analysis of M-cell marker expression in conventional epithelia from the VE and CE of mice injected with GST (control) or GST-RANKL. Results were normalized to Gapdh expression and are presented relative to the expression in the ileal epithelium from GST-treated mice. Data shown are mean values from three independent experiments (error bars indicate standard deviation). ***p < 0.005, **p < 0.01, *p < 0.05; p values were calculated with the Student’s t-test (n = 3 biologically independent experiments). c, d Nuclear translocation activities of RelB and p52 following RANKL stimulation are enhanced in Opg−/− mice. c Western blot analysis of p100/p52 and RelB in the VE and CE of mice injected with GST or GST-RANKL. Rpt4, a subunit of the 26S proteasome, was used as an internal control for the cytoplasmic fraction. Lamin A/C (Lamin) was used as an internal control for the nuclear fraction. Data are representative of two independent experiments. d Right, single confocal planes of the cecal FAE from WT and Opg−/− mice. FAE monolayers were stained with anti-RelB (green) and anti-Spi-B (red) antibodies and with Hoechst 33342. Left, a bar graph summarizing the proportions of RelB-positive cells among total numbers of M cells (Spi-B-positive cells). ***p < 0.005; p values were calculated with the Student’s t-test (n = 4 biologically independent experiments). Scale bars: 20 µm. The source data underlying panels b and d and non-cropped scan images of western blotting (c) are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932605), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7204-SP

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NBP2-37458

Spi-B Antibody (4G5) - BSA Free

(1 Publication)

0.1 ml

NBP2-37458

Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of paraffin-embedded esophageal cancerwith DAB staining.

Flow Cytometry: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).

Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.

ELISA: Spi-B Antibody (4G5) [NBP2-37458] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37458-0.025ml

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NBP1-70345

Spi-B Antibody

0.1 mg

NBP1-70345

Western Blot: SPIB Antibody [NBP1-70345] - Analysis of Spi-B in human Daudi lysate in the 1) absence and 2) presence of immunizing peptide and 3) on mouse RAW lysate using Spi-B polyclonal antibody at 4 ug/ml.

Reactivity:

Hu, Mu, Bv, Ca, Eq, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Bv, Ca, Eq, Pm, Pm

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6715A

Alt Catalog# IMG-6715A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB7576

Spi-B Antibody (769653)

100 μg

MAB7576

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Spi-B Monoclonal Antibody (Catalog # MAB7576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Spi-B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7576-SP

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NBP2-98958

Spi-B Antibody

100 ul

NBP2-98958

Immunoprecipitation: Spi-B Antibody [NBP2-98958] - Spi-B was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate 4 ul anti-Spi-B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Spi-B rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 44 kDa

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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