SAFB Antibodies
We offer SAFB Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each SAFB Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SAFB Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the SAFB Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SAFB polyclonal antibodies and browse our SAFB monoclonal antibody catalog.
Alternate Names for SAFB Antibodies
anti-SAFB antibody, anti-glutathione S-transferase fusion protein antibody, anti-HAP antibody, anti-heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein antibody, anti-HETDKFZp779C1727 antibody, anti-Hsp27 ERE-TATA binding protein antibody, anti-HSP27 ERE-TATA-binding protein antibody, anti-HSP27 estrogen response element-TATA box-binding protein antibody, anti-SAF-B antibody, anti-SAF-B1 antibody, anti-SAFB1SAB-B1 antibody, anti-scaffold attachment factor B antibody, anti-scaffold attachment factor B1 antibody
9 Results for "safb" in Primary Antibodies
![Western Blot: SAFB Antibody [NB100-2593] - Detection of Human and Mouse SAFB1 on HeLa whole cell lysate using NB100-2593. SAFB1 was also immunoprecipitated by rabbit anti-SAFB1 antibody NB100-2592.](http://images.novusbio.com/fullsize/SAFB-Antibody-Western-Blot-NB100-2593-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SAFB Antibody [NB100-2593] - Detection of Human and Mouse SAFB1 on HeLa whole cell lysate using NB100-2593. SAFB1 was also immunoprecipitated by rabbit anti-SAFB1 antibody NB100-2592.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: SAFB Antibody [NB100-2593] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SAFB1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-NB100-2593-img0005.jpg)
![Immunohistochemistry-Paraffin: SAFB Antibody [NB100-2593] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-SAFB1 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-Paraffin-NB100-2593-img0001.jpg)
![Immunohistochemistry-Paraffin: SAFB Antibody [NB100-2593] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-SAFB1 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-Paraffin-NB100-2593-img0002.jpg)
![Immunohistochemistry: SAFB Antibody [NB100-2593] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SAFB1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-NB100-2593-img0004.jpg)
![Western Blot: SAFB Antibody (5A11) [H00006294-M04] - Analysis of SAFB expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/SAFB-Antibody-5A11-Western-Blot-H00006294-M04-img0007.jpg "Western Blot: SAFB Antibody (5A11) [H00006294-M04] - Analysis of SAFB expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: SAFB Antibody (5A11) [H00006294-M04] - Analysis of monoclonal antibody to SAFB on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/SAFB-Antibody-5A11-Immunocytochemistry-Immunofluorescence-H00006294-M04-img0005.jpg)
![Immunohistochemistry-Paraffin: SAFB Antibody (5A11) [H00006294-M04] - Analysis of monoclonal antibody to SAFB on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/SAFB-Antibody-5A11-Immunohistochemistry-Paraffin-H00006294-M04-img0006.jpg)
![ELISA: SAFB Antibody (5A11) [H00006294-M04] - Detection limit for recombinant GST tagged SAFB is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SAFB-Antibody-5A11-ELISA-H00006294-M04-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: SAFB Antibody [NBP3-38485] - Immunofluorescence analysis of U-2 OS cells using SAFB Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38485_rabbit-safb-pab-1012202412061.jpg "Immunocytochemistry/ Immunofluorescence: SAFB Antibody [NBP3-38485] - Immunofluorescence analysis of U-2 OS cells using SAFB Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: SAFB Antibody [NBP3-38485] - Western blot analysis of various lysates using SAFB Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38485_rabbit-safb-pab-10122024121072.jpg)
![Immunohistochemistry: SAFB Antibody [NBP3-38485] - Immunohistochemistry analysis of paraffin-embedded Human colon using SAFB Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38485_rabbit-safb-pab-101220241154552.jpg)
![Western Blot: SAFB Antibody (JE64-94) [NBP3-32837] - Western blot analysis of SAFB on A431 cell lysates with Rabbit anti-SAFB antibody (NBP3-32837) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 103 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32837) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32837_rabbit-safb-mab-je64-94-288202412461634.jpg "Western Blot: SAFB Antibody (JE64-94) [NBP3-32837] - Western blot analysis of SAFB on A431 cell lysates with Rabbit anti-SAFB antibody (NBP3-32837) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 103 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32837) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SAFB Antibody (JE64-94) [NBP3-32837] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SAFB antibody (NBP3-32837) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32837) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32837_rabbit-safb-mab-je64-94-298202414333691.jpg)
![Immunocytochemistry/ Immunofluorescence: SAFB Antibody (JE64-94) [NBP3-32837] - Immunocytochemistry analysis of NCI-H441 cells labeling SAFB with Rabbit anti-SAFB antibody (NBP3-32837) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SAFB antibody (NBP3-32837) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32837_rabbit-safb-mab-je64-94-2920241023421.jpg)
![Western Blot: SAFB Antibody (6F7) [NB200-332] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5% SDS-PAGE SAFB antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SAFB-Antibody-6F7-Western-Blot-NB200-332-img0004.jpg "Western Blot: SAFB Antibody (6F7) [NB200-332] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5% SDS-PAGE SAFB antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SAFB Antibody (6F7) [NB200-332] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SAFB-Antibody-6F7-Immunocytochemistry-Immunofluorescence-NB200-332-img0002.jpg)
![Immunohistochemistry: SAFB Antibody (6F7) [NBP3-27940] - Immunohistochemistry staining of SAFB in brain cortex tissue using SAFB monoclonal Antibody.](http://images.novusbio.com/fullsize/nbp3-27940_mouse-safb-mab-6f7-2711202420312952.jpg "Immunohistochemistry: SAFB Antibody (6F7) [NBP3-27940] - Immunohistochemistry staining of SAFB in brain cortex tissue using SAFB monoclonal Antibody.")
![Western Blot: SAFB Antibody (SR1200) [NBP3-22591] - Western blot analysis using NBP3-22591 in 293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22591_rabbit-safb-mab-sr1200-5720231531245.jpg "Western Blot: SAFB Antibody (SR1200) [NBP3-22591] - Western blot analysis using NBP3-22591 in 293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: SAFB Antibody [NBP1-83265] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83265-img0005.jpg "Immunocytochemistry/Immunofluorescence: SAFB Antibody [NBP1-83265] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: SAFB Antibody [NBP1-83265] - Staining of human cervix, uterine shows moderate nuclear and cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-Paraffin-NBP1-83265-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SAFB Antibody [NBP1-83266] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83266-img0007.jpg "Immunocytochemistry/Immunofluorescence: SAFB Antibody [NBP1-83266] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: SAFB Antibody [NBP1-83266] - Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-Paraffin-NBP1-83266-img0006.jpg)