PRPS2 Antibodies
We offer PRPS2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, RNA Inhibition, Western Blot. Each PRPS2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PRPS2 Antibodies can be used in a variety of model species: Human. Use the list below to choose the PRPS2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PRPS2 polyclonal antibodies and browse our PRPS2 monoclonal antibody catalog.
Alternate Names for PRPS2 Antibodies
anti-PRPS2 antibody, anti-EC 2.7.6.1 antibody, anti-Phosphoribosyl pyrophosphate synthase II antibody, anti-phosphoribosyl pyrophosphate synthetase 2 antibody, anti-PPRibP antibody, anti-PPRibP synthetase antibody, anti-PRS-II antibody, anti-PRSII antibody, anti-ribose-phosphate diphosphokinase 2 antibody, anti-ribose-phosphate pyrophosphokinase 2 antibody
8 Results for "prps2" in Primary Antibodies
![Western Blot: PRPS2 Antibody [NBP1-31435] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE PRPS2 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Western-Blot-NBP1-31435-img0005.jpg "Western Blot: PRPS2 Antibody [NBP1-31435] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE PRPS2 antibody, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PRPS2 Antibody [NBP1-31435] - Analysis of HaLa, using NBP1-31435 at 1:200 dilution.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31435-img0003.jpg)
![Immunohistochemistry: PRPS2 Antibody [NBP1-31435] - Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), and with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, and in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 x 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 x 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 x 1 = 1. Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunohistochemistry-NBP1-31435-img0007.jpg)
![Immunohistochemistry-Paraffin: PRPS2 Antibody [NBP1-31435] - Paraffin-embedded Hep3B xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunohistochemistry-Paraffin-NBP1-31435-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: PRPS2 Antibody [NBP1-31435] - The effects of PRPS2 knockdown confirmed by qRT-PCR (top) and Western blotting (bottom). Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Knockdown-Validated-NBP1-31435-img0006.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 decreased prostate cancer cell growth. (A) The effects of PRPS2 knockdown confirmed by qRT-PCR (top) & Western blotting (bottom). (B) In the CCK‑8 assay, cell viability was decreased in si‑PRPS2 compared with NC. (C) In the plate colony formation assay, colony formation was obviously decreased in si-PRPS2 cells transfected. ***P < 0.001. NC: negative controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-310202415345324.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 inhibits xenograft tumor growth in vivo. (A) PRPS2 shRNA effectively blocked its protein expression in PC-3 cells. Cells were stably transfected with scramble (sh-Ctrl) or PRPS2 shRNA (sh-PRPS2). PRPS2 protein levels were normalized to alpha -Tubulin. (B) Gross observation of xenograft tumor size in NOD/SCID mice. (C & D) Silencing of PRPS2 inhibited the tumor growth, including tumor volume (P < 0.001) & weight (P = 0.028, n = 6). (E) H&E‑stained paraffin‑embedded sections obtained from xenografts of PC-3 cells. (F) Top: Immunohistochemical analysis of Ki-67 in the xenografts; Bottom: The apoptosis in tumor tissues was evaluated by TUNEL assay (×200). Graphical illustrated the quantification of Ki-67 & TUNEL positive cells percentage. ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-310202416163792.jpg)
![Western Blot: PRPS2 Antibody [NBP1-31435] - PRPS2 expression in prostate cancer tissues & prostate cancer cell lines. (A) Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), & with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, & in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 & its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 & its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 & its total protein expression score is 1 × 1 = 1. (B) Western blotting & real-time qPCR analysis of PRPS2 protein & mRNA expression in prostate cancer cell lines & normal prostate epithelial cell lines (RWPE-1). (*P < 0.05; **P < 0.01; ***P < 0.001). PRPS2: Phosphoribosyl pyrophosphate synthetase 2; TMA: tissue microarray. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-3102024161890.jpg)
![Western Blot: PRPS2 Antibody [NBP1-57669] - Titration: 0.2-1 ug/ml Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Western-Blot-NBP1-57669-img0003.jpg "Western Blot: PRPS2 Antibody [NBP1-57669] - Titration: 0.2-1 ug/ml Positive Control: HepG2 cell lysate.")
![Immunohistochemistry-Paraffin: PRPS2 Antibody [NBP1-57669] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunohistochemistry-Paraffin-NBP1-57669-img0004.jpg)
![Western Blot: PRPS2 Antibody [NBP2-56594] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Western-Blot-NBP2-56594-img0002.jpg "Western Blot: PRPS2 Antibody [NBP2-56594] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.")
![Immunocytochemistry/Immunofluorescence: PRPS2 Antibody [NBP2-56594] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56594-img0001.jpg)
![Western Blot: PRPS2 Antibody [NBP1-56666] - Jurkat cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Western-Blot-NBP1-56666-img0003.jpg "Western Blot: PRPS2 Antibody [NBP1-56666] - Jurkat cell lysate, concentration 1.25ug/ml.")
![Western Blot: PRPS2 Antibody [NBP3-35260] - Western blot analysis of various lysates using PRPS2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35260_rabbit-prps2-pab-91220241519585.jpg "Western Blot: PRPS2 Antibody [NBP3-35260] - Western blot analysis of various lysates using PRPS2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: PRPS2 Antibody (4C1) [H00005634-M02] - Analysis of PRPS2 expression in transfected 293T cell line by PRPS2 monoclonal antibody (M02), clone 4C1.Lane 1: PRPS2 transfected lysate(34.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PRPS2-Antibody-4C1-Western-Blot-H00005634-M02-img0004.jpg "Western Blot: PRPS2 Antibody (4C1) [H00005634-M02] - Analysis of PRPS2 expression in transfected 293T cell line by PRPS2 monoclonal antibody (M02), clone 4C1.Lane 1: PRPS2 transfected lysate(34.8 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: PRPS2 Antibody (4C1) [H00005634-M02] - Analysis of PRPS2 over-expressed 293 cell line, cotransfected with PRPS2 Validated Chimera RNAi ( Cat # H00005634-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRPS2 monoclonal antibody (M02), clone 4C1 (Cat # H00005634-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PRPS2-Antibody-4C1-Western-Blot-H00005634-M02-img0003.jpg)
![Western Blot: PRPS2 Antibody [NBP3-35261] - Western blot analysis of various lysates using PRPS2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35261_rabbit-prps2-pab-912202415175222.jpg "Western Blot: PRPS2 Antibody [NBP3-35261] - Western blot analysis of various lysates using PRPS2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: PRPS2 Antibody [NBP1-57670] - Jurkat cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Western-Blot-NBP1-57670-img0003.jpg "Western Blot: PRPS2 Antibody [NBP1-57670] - Jurkat cell lysate, concentration 1.25ug/ml.")