PKM1 Antibodies
We offer PKM1 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot. Each PKM1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PKM1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the PKM1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PKM1 polyclonal antibodies.
Alternate Names for PKM1 Antibodies
anti-PKM1 antibody, anti-PKM antibody, anti-PK1 antibody, anti-Pyruvate kinase isozymes M1 antibody, anti-Pyruvate kinase M1 antibody
3 Results for "pkm1" in Primary Antibodies
![Western Blot: PKM1 Antibody [NBP2-14833] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions as Figure 1a at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM1-Antibody-Western-Blot-NBP2-14833-img0008.jpg "Western Blot: PKM1 Antibody [NBP2-14833] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions as Figure 1a at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: PKM1 Antibody [NBP2-14833] - PKM1 antibody was tested in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PKM1-Antibody-Immunohistochemistry-Paraffin-NBP2-14833-img0002.jpg)
![Western Blot: PKM1 Antibody [NBP2-14833] - PKM1 antibody was tested in 1. human skeletal muscle 2. mouse skeletal muscle 3. human brain 4. mouse brain and 5. human heart cell lysate.](http://images.novusbio.com/fullsize/PKM1-Antibody-Western-Blot-NBP2-14833-img0003.jpg)
![Western Blot: PKM1 Antibody [NBP2-14833] - Detection of PKM1 on HEK293T and U-87 cells. Lane 1: HEK293T cells (major isoform is PKM2, human). Lane 2: HEK293T cells with plasmid overexpressing mouse PKM1. Lane 3: HEK293T cells with plasmid overexpressing mouse PKM2. Lane 4: U-87 cells with control shRNA (major isoform is PKM2, human). Lane 5: U-87 cells with PTB/A1/A2 shRNAs (major isoform is PKM1, human). Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/PKM1-Antibody-Western-Blot-NBP2-14833-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PKM1 Antibody [NBP2-14833] - Expression of PTB1, PKM1, and PKM2 proteins at 72 h after transfection of DLD-1, RD or KYM-1 cells with miR-133b (10, 20 nM). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM1-Antibody-Western-Blot-NBP2-14833-img0006.jpg)
![Western Blot: PKM1 Antibody [NBP2-14833] - Expression profile of PKM isoforms in various cancer cell lines, control cell lines, and cells in primary culture. PKM1 and PKM2 were detected by Western blotting under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2a. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKM1-Antibody-Western-Blot-NBP2-14833-img0007.jpg)
![Simple Western: PKM1 Antibody [NBP2-14833] - Simple Western lane view shows a specific band for PKM1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PKM1-Antibody-Simple-Western-NBP2-14833-img0005.jpg)
![Immunohistochemistry-Paraffin: PKM1 Antibody - BSA Free [NBP2-14833] - Expression of PKM1 & PKM2 in clinical colorectal cancer samples.(a) The protein expression of PKM1 & PKM2 in clinical specimens of cancer tumor (T) & the adjacent normal tissues (N) is shown. PKM1 & PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S3b. (b–d) Immunohistochemical staining of normal colon tissue adjacent to tumor tissue of case 10. Results of H&E staining (b), staining with anti-PKM1 antibody (c), & staining with anti-PKM2 (d) are shown. The boxed regions in “c” & “d” are enlarged in the images below. (e–h) Immunohistochemical staining of clinical colorectal cancer tissue specimen of representative case 3. H&E-stained section with normal tissue (upper right corner) neighboring the tumor area in the section is shown (e), along with the same section stained with anti-PKM2 antibody (f). Enlarged views of boxed areas in “f” show normal colorectal crypt in mucosa (g) & tumor area (h) stained with anti-PKM2 antibody. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-31020241612233.jpg)
![Western Blot: PKM1 Antibody - BSA Free [NBP2-14833] - (a) Luciferase activities after co-transfection of DLD-1 cells w/ control or miR-124 (wild-type or mutant-type) pMIR vectors having predictive miR-124 binding site in 3′UTR of PTB1. Upper panel region of 3′-UTR of human PTB1 mRNA complementary to mature miR-124. Box indicates predicted binding sites for miR-124. (b) Same as “a” except miR-133b used. (c) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB9 or IMR-32 cells w/ miR-124 (10, 20 or 40 nM). (d) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, RD or KYM-1 cells w/ miR-133b (10, 20 nM). (e) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB-9 or RD cells w/ siR-PTB1 (2, 5 nM). (f) Effect of combined treatment of DLD-1 cells w/ antagomiR-124 & miR-124 or antagomiR-133b & miR-133b. DLD-1 cells transfected w/ non-specific control, miR-124/miR-133b (10 nM), miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (5 nM) or miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (10 nM). Expression level of PTB1 assessed at 48 h after transfection. The full-length blots are presented in Supplementary Figure S3a. (g) IF of PKM1 (upper panels) & PKM2 (lower panels) at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM) or miR-133b (20 nM). Left panels, treatment w/ control miRNA; middle panels, treatment w/ miR-124; right panels, treatment w/ miR-133b. PKM1 or PKM2 is stained red, & nuclei are stained blue. (h) Lactate production measured at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM), miR-133b (20 nM) or siR-PTB1 (5 nM). Results are presented as mean± SD (* P < 0.05; ** P < 0.01; *** P < 0.001; N.S., not statistically significant). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-31020241612196.jpg)
![Immunocytochemistry/ Immunofluorescence: PKM1 Antibody - BSA Free [NBP2-14833] - (a) Luciferase activities after co-transfection of DLD-1 cells w/ control or miR-124 (wild-type or mutant-type) pMIR vectors having predictive miR-124 binding site in 3′UTR of PTB1. Upper panel region of 3′-UTR of human PTB1 mRNA complementary to mature miR-124. Box indicates predicted binding sites for miR-124. (b) Same as “a” except miR-133b used. (c) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB9 or IMR-32 cells w/ miR-124 (10, 20 or 40 nM). (d) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, RD or KYM-1 cells w/ miR-133b (10, 20 nM). (e) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB-9 or RD cells w/ siR-PTB1 (2, 5 nM). (f) Effect of combined treatment of DLD-1 cells w/ antagomiR-124 & miR-124 or antagomiR-133b & miR-133b. DLD-1 cells transfected w/ non-specific control, miR-124/miR-133b (10 nM), miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (5 nM) or miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (10 nM). Expression level of PTB1 assessed at 48 h after transfection. The full-length blots are presented in Supplementary Figure S3a. (g) IF of PKM1 (upper panels) & PKM2 (lower panels) at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM) or miR-133b (20 nM). Left panels, treatment w/ control miRNA; middle panels, treatment w/ miR-124; right panels, treatment w/ miR-133b. PKM1 or PKM2 is stained red, & nuclei are stained blue. (h) Lactate production measured at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM), miR-133b (20 nM) or siR-PTB1 (5 nM). Results are presented as mean± SD (* P < 0.05; ** P < 0.01; *** P < 0.001; N.S., not statistically significant). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-31020241613613.jpg)
![Western Blot: PKM1 Antibody - BSA Free [NBP2-14833] - (a) Luciferase activities after co-transfection of DLD-1 cells w/ control or miR-124 (wild-type or mutant-type) pMIR vectors having predictive miR-124 binding site in 3′UTR of PTB1. Upper panel region of 3′-UTR of human PTB1 mRNA complementary to mature miR-124. Box indicates predicted binding sites for miR-124. (b) Same as “a” except miR-133b used. (c) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB9 or IMR-32 cells w/ miR-124 (10, 20 or 40 nM). (d) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, RD or KYM-1 cells w/ miR-133b (10, 20 nM). (e) Expression of PTB1, PKM1, & PKM2 proteins at 72 h after transfection of DLD-1, NB-9 or RD cells w/ siR-PTB1 (2, 5 nM). (f) Effect of combined treatment of DLD-1 cells w/ antagomiR-124 & miR-124 or antagomiR-133b & miR-133b. DLD-1 cells transfected w/ non-specific control, miR-124/miR-133b (10 nM), miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (5 nM) or miR-124/miR-133b (10 nM) + antagomiR-124/antagomiR-133b (10 nM). Expression level of PTB1 assessed at 48 h after transfection. The full-length blots are presented in Supplementary Figure S3a. (g) IF of PKM1 (upper panels) & PKM2 (lower panels) at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM) or miR-133b (20 nM). Left panels, treatment w/ control miRNA; middle panels, treatment w/ miR-124; right panels, treatment w/ miR-133b. PKM1 or PKM2 is stained red, & nuclei are stained blue. (h) Lactate production measured at 48 h after transfection of DLD-1 cells w/ miR-124 (20 nM), miR-133b (20 nM) or siR-PTB1 (5 nM). Results are presented as mean± SD (* P < 0.05; ** P < 0.01; *** P < 0.001; N.S., not statistically significant). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-310202416113524.jpg)
![Western Blot: PKM1 Antibody - BSA Free [NBP2-14833] - Expression of PKM1 & PKM2 in clinical colorectal cancer samples.(a) The protein expression of PKM1 & PKM2 in clinical specimens of cancer tumor (T) & the adjacent normal tissues (N) is shown. PKM1 & PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S3b. (b–d) Immunohistochemical staining of normal colon tissue adjacent to tumor tissue of case 10. Results of H&E staining (b), staining with anti-PKM1 antibody (c), & staining with anti-PKM2 (d) are shown. The boxed regions in “c” & “d” are enlarged in the images below. (e–h) Immunohistochemical staining of clinical colorectal cancer tissue specimen of representative case 3. H&E-stained section with normal tissue (upper right corner) neighboring the tumor area in the section is shown (e), along with the same section stained with anti-PKM2 antibody (f). Enlarged views of boxed areas in “f” show normal colorectal crypt in mucosa (g) & tumor area (h) stained with anti-PKM2 antibody. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-3102024161364.jpg)
![Western Blot: PKM1 Antibody - BSA Free [NBP2-14833] - Characterization of NOX4 & PKM2 in human RCC tumors & adjacent tissue. a Mitochondrial fractions were prepared from human tumors (T) or uninvolved adjacent tissue (N). NOX4 expression was examined by western blot analysis. Prohibitin was probed as a mitochondrial marker & loading control. b Quantitation of NOX4 distribution in the mitochondrial fraction from a. The results are expressed as the means using one-way ANOVA with Tukey’s post hoc test where ± S.E.M. *p < 0.05 compared to normal (N). c Mitochondria fractions were prepared from RCC tumors & NADPH-dependent superoxide generation was examined in the presence (+) or absence (−) of ATP. The results are from eight tumors & are expressed as the means using one-way ANOVA with Tukey’s post hoc test where ±S.E.M. **p < 0.01 is compared to without (−) ATP. d PKM2 & PKM1 expression was examined by western blot analysis in lysates prepared from human tumors (T) or uninvolved adjacent tissue (N) from the same patient. Actin as loading control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29051480), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14833_rabbit-polyclonal-pkm1-antibody-310202416121963.jpg)
![Western Blot: PKM1 Antibody (PSH04-37) [NBP3-32825] - Western blot analysis of PKM1 on different lysates with Rabbit anti-PKM1 antibody (NBP3-32825) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Mouse heart tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (negative) (40 ug/Lane) Predicted band size: 58 kDa Observed band size: 55 kDa Exposure time: 14 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32825) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32825_rabbit-pkm1-mab-psh04-37-288202412442963.jpg "Western Blot: PKM1 Antibody (PSH04-37) [NBP3-32825] - Western blot analysis of PKM1 on different lysates with Rabbit anti-PKM1 antibody (NBP3-32825) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Mouse heart tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (negative) (40 ug/Lane) Predicted band size: 58 kDa Observed band size: 55 kDa Exposure time: 14 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32825) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PKM1 Antibody (PSH04-37) [NBP3-32825] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-PKM1 antibody (NBP3-32825) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32825) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32825_rabbit-pkm1-mab-psh04-37-298202414262689.jpg)
![Immunocytochemistry/ Immunofluorescence: PKM1 Antibody (PSH04-37) [NBP3-32825] - Immunocytochemistry analysis of HCT 116 cells labeling PKM1 with Rabbit anti-PKM1 antibody (NBP3-32825) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PKM1 antibody (NBP3-32825) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32825_rabbit-pkm1-mab-psh04-37-29202410234284.jpg)
![Flow Cytometry: PKM1 Antibody (PSH04-37) [NBP3-32825] - Flow cytometric analysis of HCT 116 cells labeling PKM1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32825, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32825_rabbit-pkm1-mab-psh04-37-2920241637219.jpg)