Phosphothreonine Antibodies
We offer Phosphothreonine Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each Phosphothreonine Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Phosphothreonine Antibodies can be used in a variety of model species: Fungi. Use the list below to choose the Phosphothreonine Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Phosphothreonine polyclonal antibodies and browse our Phosphothreonine monoclonal antibody catalog.
Alternate Names for Phosphothreonine Antibodies
anti-Phosphothreonine antibody
6 Results for "phosphothreonine" in Primary Antibodies
![Western Blot: Phosphothreonine Antibody [NB110-96896] - Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896). Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896) at 1:1000. Left: Treated with Vanadium, Right: Non-treated..](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-Western-Blot-NB110-96896-img0007.jpg "Western Blot: Phosphothreonine Antibody [NB110-96896] - Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896). Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896) at 1:1000. Left: Treated with Vanadium, Right: Non-treated..")
![Immunocytochemistry/Immunofluorescence: Phosphothreonine Antibody [NB110-96896] - Human HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96896-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Phosphothreonine Antibody [NB110-96896] - Human HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96896-img0004.jpg)
![Immunoprecipitation: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] - Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by clone RM102 rabbit Anti-Phosphothreonine mAb, at 1:500 dilution, and then blotted with RM102. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM102.](http://images.novusbio.com/fullsize/nbp3-25980_rabbit-phosphothreonine-mab-rm102-azide-and-bsa-free-22620242311086.jpg "Immunoprecipitation: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] - Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by clone RM102 rabbit Anti-Phosphothreonine mAb, at 1:500 dilution, and then blotted with RM102. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM102.")
![Immunocytochemistry/ Immunofluorescence: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] - Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using Clone RM102 at 1:500 dilution followed by a PE conjugated secondary antibody (red) and DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-25980_rabbit-phosphothreonine-mab-rm102-azide-and-bsa-free-226202422174423.jpg)
![Western Blot: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] - Western blot of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using Clone RM102 at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-25980_rabbit-phosphothreonine-mab-rm102-azide-and-bsa-free-2462024132611.jpg)
![Multiplex Immunoassay: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] - Clone RM102 recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.](http://images.novusbio.com/fullsize/nbp3-25980_rabbit-phosphothreonine-mab-rm102-azide-and-bsa-free-22620242311037.jpg)
![Western Blot: Phosphothreonine Antibody (RM102) [NBP2-77398] - Western blot of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using NBP2-77398.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-RM102-Western-Blot-NBP2-77398-img0002.jpg "Western Blot: Phosphothreonine Antibody (RM102) [NBP2-77398] - Western blot of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using NBP2-77398.")
![Immunocytochemistry/Immunofluorescence: Phosphothreonine Antibody (RM102) [NBP2-77398] - Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using NBP2-77398 (followed by a PE conjugated secondary antibody, red) and DAPI (blue).](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-RM102-Immunocytochemistry-Immunofluorescence-NBP2-77398-img0001.jpg)
![Multiplex Immunoassay: Phosphothreonine Antibody (RM102) [NBP2-77398] - NBP2-77398 recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-RM102-Multiplex-Immunoassay-NBP2-77398-img0004.jpg)
![Immunoprecipitation: Phosphothreonine Antibody (RM102) [NBP2-77398] - Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by NBP2-77398, and then blotted with NBP2-77398. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by NBP2-77398.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-RM102-Immunoprecipitation-NBP2-77398-img0003.jpg)
![Western Blot: Phosphothreonine Antibody (18F6) [NB600-878] - Phospho Threonine monoclonal antibody, clone 18F6 is shown to detect threonine phosphorylation of proteins in a lysate from A-431 cells stimulated with EGF.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-18F6-Western-Blot-NB600-878-img0003.jpg "Western Blot: Phosphothreonine Antibody (18F6) [NB600-878] - Phospho Threonine monoclonal antibody, clone 18F6 is shown to detect threonine phosphorylation of proteins in a lysate from A-431 cells stimulated with EGF.")
![ELISA: Phosphothreonine Antibody (18F6) [NB600-878] - was tested against BSA conjugates of phosphothreonine (grey squares), phosphotyrosine (black triangles) and phosphoserine (open circles). Each well was coated with 0.1 ug of conjugate. The starting dilution of antibody was 1/1000 and each point on the X axis represents a 2-fold dilution. For detection, an HRP-conjugated Goat anti-Mouse IgG was used as secondary antibody, with a TMB substrate. Absorbance is represented on the Y axis.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-18F6-ELISA-NB600-878-img0004.jpg)
![Western Blot: Phosphothreonine Antibody [NBP1-78035] - Goat anti Glucoamylase antibody was used to detect purified glucoamylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-Western-Blot-NBP1-78035-img0002.jpg "Western Blot: Phosphothreonine Antibody [NBP1-78035] - Goat anti Glucoamylase antibody was used to detect purified glucoamylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane.")
![Western Blot: Phosphothreonine Antibody [NBP1-78035] - shown to detect phosphorylated proteins present in mouse spleen cell lysates. Left Lane: extract of spleen from mouse treated with vanadium. Right lane: extract of spleen from untreated mouse.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-Western-Blot-NBP1-78035-img0001.jpg)
![Western Blot: Phosphothreonine Antibody (6K2H0) [NBP3-33576] - Western blot analysis of lysates from NIH 3T3 or NIH 3T3 treat with Calyculin A, using Phosphothreonine Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33576_rabbit-phosphothreonine-mab-6k2h0-912202415171146.jpg "Western Blot: Phosphothreonine Antibody (6K2H0) [NBP3-33576] - Western blot analysis of lysates from NIH 3T3 or NIH 3T3 treat with Calyculin A, using Phosphothreonine Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: Phosphothreonine Antibody (6K2H0) [NBP3-33576] - Western blot analysis of lysates from A-431, using Phosphothreonine Rabbit mAb at 1:1000 dilution. A 431 treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33576_rabbit-phosphothreonine-mab-6k2h0-91220241512131.jpg)