Nucleolin Antibodies
We offer Nucleolin Antibodies for use in common research applications: Chromatin Immunoprecipitation, Electron Microscopy, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunomicroscopy, Immunoprecipitation, Western Blot. Each Nucleolin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Nucleolin Antibodies can be used in a variety of model species: Human, Mouse, Plant, Rat. Use the list below to choose the Nucleolin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Nucleolin polyclonal antibodies.
Alternate Names for Nucleolin Antibodies
anti-Nucleolin antibody, anti-NCL antibody, anti-C23 antibody, anti-FLJ45706 antibody, anti-FLJ59041 antibody, anti-Protein C23 antibody
27 Results for "nucleolin" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - Analysis of Nucleolin MCF-7 breast cancer cells. Image courtsey of product review by Lacey Litchfield.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-241-img0008.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - Analysis of Nucleolin MCF-7 breast cancer cells. Image courtsey of product review by Lacey Litchfield.")
![Simple Western: Nucleolin Antibody [NB600-241] - Image shows a specific band for Nucleolin in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Simple-Western-NB600-241-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - Endogenous nuclear nucleolin-COUP-TFII interaction in MCF-7 and T47D cells.NE (200A ug protein) from MCF-7 cells. Immunofluorescent staining of endogenous COUP-TFII (green) and nucleolin (red) in the nuclei (Hoechst, blue) of MCF-7 cells. Merged images are shown at the right. Bar is 10A um. Image collected and cropped by Citeab from the following publication (Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor transcription in breast cancer cells. PLoS One (2012) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-241-img0012.jpg)
![Western Blot: Nucleolin Antibody [NB600-241] - Detection of nucleolin in crude PD31 nuclear extracts.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Western-Blot-NB600-241-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-241-img0011.jpg)
![Immunohistochemistry: Nucleolin Antibody [NB600-241] - Detection of Nucleolin in human tonsil germinal center.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunohistochemistry-NB600-241-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - In-situ immunofluorescent staining of PD31 murine pre-B cells.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-241-img0007.jpg)
![Western Blot: Nucleolin Antibody [NB600-241] - HT-LKO cells recapitulate the hallmarks of lamin A/C deficiency.(a) Quantitative PCR shows a >6-fold reduction (expressed as log2 fold change of ddCt value) of LMNA transcripts in different LKO colonies as compared to HT-WT clones; (b) Quantitative immunofluorescence shows a dramatic reduction of A-type lamin levels in HT-LKO colonies, approximating background levels (dotted white line); (c) Western blot for lamin A/C reveals the absence of both proteins in HT-LKO cells; (d) Immunostaining & nuclear counterstaining of HT-LKO cells reveals their aberrant nuclear morphology, virtual absence of lamin A (green) & local depletion of lamin B (red) (arrowheads) as opposed to HT-WT cells; (e) Time-lapse recordings after H2B-GFP transfection illustrate increased nuclear plasticity of HT-LKO vs. HT-WT cells, as evidenced by their larger projected area (Σ Area) & contour changes (Σ Outline) across time; (f) HT-LKO nuclei have a lower average nuclear circularity (dotted white line indicates a circularity of 0.85) & higher nuclear circularity fluctuations (average + 95% confidence interval) than HT-WT cells; The Y-axis has been cropped for clarity; (g) This translates into a significantly larger coefficient of variation (CoV) for the circularity across time (p < 0.001). (h) Nuclear ruptures occur more frequently in HT-LKO cells than in HT-WT cells (p < 0.001); (i) Representative montages of the NLS channel of a C4 HT-WT & L2 HT-LKO cell, with corresponding NLS/H2B ratio measurements (grey-coded bar plots). The moments of rupture events are indicated as red dots. Bar graphs reflect mean ± standard error (n = number of tracks). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27461848), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-241_rabbit-polyclonal-nucleolin-antibody-310202416165620.jpg)
![Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Western-Blot-NBP3-11037-img0003.jpg "Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0010.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Human tonsil stained with Nucleolin Antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0001.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Analysis of human cells administered in pig brain using Nucleolin antibody. Antibody did NOT stain porcine tissue allowing the presence of human cells to be determined. Primary antibody dilution 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Immunohistochemistry-Paraffin-NBP3-11037-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is DAPI (Blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0008.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is DAPI (Blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0003.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Flow Cytometry of Human Nucleolin Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Flow-Cytometry-NBP2-44612-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Skin stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0004.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Human Nucleolin Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Flow-Cytometry-NBP2-44612-img0001.jpg)
![Western Blot: Nucleolin Antibody [NB100-1920] - Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Nucleolin antibody NB100-1920 (aNCL) used to probe western blot is indicated to the left of the image. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005121#sec002) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Western-Blot-NB100-1920-img0010.jpg "Western Blot: Nucleolin Antibody [NB100-1920] - Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Nucleolin antibody NB100-1920 (aNCL) used to probe western blot is indicated to the left of the image. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005121#sec002) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB100-1920] - Detection of Nucleolin (green) in Hela cells detected with a Dylight-488 conjugated secondary antibody. Nuclei are counter stained with Hoechst 33258.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1920-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody [NB100-1920] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human colon cancer using 1:750 conc. of Nucleolin antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed in normal cells and nuclear staining was observed in cancer cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunohistochemistry-Paraffin-NB100-1920-img0009.jpg)
![Western Blot: Nucleolin Antibody [NB100-1920] - Analysis of Nucleolin in HeLa nuclear extracts.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Western-Blot-NB100-1920-img0006.jpg)
![Western Blot: Nucleolin Antibody (NCL/902) [NBP2-44610] - A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Western-Blot-NBP2-44610-img0009.jpg "Western Blot: Nucleolin Antibody (NCL/902) [NBP2-44610] - A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)")
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0016.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Formalin-fixed, paraffin-embedded human colon stained with Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0021.jpg)
![Flow Cytometry: Nucleolin Antibody (NCL/902) [NBP2-44610] - Flow Cytometry of Human Nucleolin Ag on Jurkat cells. Grey: Isotype Control; Green: FITC-labeled; Purple: APC-Labeled; Turquoise: AF700 Labeled Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Flow-Cytometry-NBP2-44610-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - HeLa cells stained with AF488 labeled Nucleolin Antibody (NCL/902) Green: AF488-labeled Ab. Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Antibody (NCL/902)(Green) Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0015.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Bladder carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0003.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Skin stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Uterus stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0018.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Formalin-fixed, paraffin-embedded human Uterus stained with Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44613-img0009.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0010.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (364-5 + NCL/902).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Flow-Cytometry-NBP2-44613-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44613-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0003.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Testicular Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902)](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Uterus stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0006.jpg)
![Western Blot: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Western-Blot-NBP3-11038-img0001.jpg "Western Blot: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0003.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0006.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) [NBP2-44611] - Human Tonsil stained with Nucleolin Monoclonal Antibody (SPM614).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunohistochemistry-Paraffin-NBP2-44611-img0001.jpg)
![Flow Cytometry: Nucleolin Antibody (SPM614) [NBP2-44611] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (SPM614).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Flow-Cytometry-NBP2-44611-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Antibody (SPM614)(Green) Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0005.jpg)
![Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-278202415212410.jpg "Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32668) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-298202414333643.jpg)
![Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunocytochemistry analysis of Hela cells labeling Nucleolin with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-29202410334079.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48319-img0003.jpg "Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48319-img0001.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48319-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NBP1-88271] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88271-img0004.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NBP1-88271] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.")
![Immunohistochemistry-Paraffin: Nucleolin Antibody [NBP1-88271] - Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunohistochemistry-Paraffin-NBP1-88271-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7338) [NBP3-13980] - Formalin-fixed, paraffin-embedded human pancreas stained with Nucleolin antibody (NCL/7338). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7338-Immunohistochemistry-Paraffin-NBP3-13980-img0002.jpg "Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7338) [NBP3-13980] - Formalin-fixed, paraffin-embedded human pancreas stained with Nucleolin antibody (NCL/7338). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7338) [NBP3-13980] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin antibody (NCL/7338). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7338-Immunohistochemistry-Paraffin-NBP3-13980-img0001.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7338) [NBP3-13980] - Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin antibody (NCL/7338). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7338-Immunohistochemistry-Paraffin-NBP3-13980-img0003.jpg)