Nrf2 Antibodies
We offer Nrf2 Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Nrf2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Nrf2 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Nrf2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Nrf2 polyclonal antibodies and browse our Nrf2 monoclonal antibody catalog.
Alternate Names for Nrf2 Antibodies
anti-Nrf2 antibody, anti-NFE2L2 antibody, anti-HEBP1 antibody, anti-NF-E2-related factor 2 antibody, anti-NFE2-related factor 2 antibody, anti-NRF2 erythroid derived 2, like 2 antibody, anti-nuclear factor (erythroid-derived 2)-like 2 antibody, anti-nuclear factor erythroid 2-related factor 2 antibody, anti-nuclear factor erythroid-derived 2-like 2 antibody
12 Results for "nrf2" in Primary Antibodies
![Hydralazine enhances NRF2 signaling in SH-SY5Y cells. c Hydralazine reduced the interaction between NRF2 and KEAP1. Interactions were measured by reciprocal Co-IPs followed by western blot analysis. *p  0.05). n = 5–6/group. * P Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nrf2 stained by Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32822_rabbit-polyclonal-nrf2-antibody-91020242021994.jpg "Hydralazine enhances NRF2 signaling in SH-SY5Y cells. c Hydralazine reduced the interaction between NRF2 and KEAP1. Interactions were measured by reciprocal Co-IPs followed by western blot analysis. *p  0.05). n = 5–6/group. * P Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nrf2 stained by Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - Nrf2 antibody [N2C2], Internal detects Nrf2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Nrf2 stained by Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32822_rabbit-polyclonal-nrf2-antibody-910202420115018.jpg)
![Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) Rat2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-32822_rabbit-polyclonal-nrf2-antibody-910202419593583.jpg)
![Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32822_rabbit-polyclonal-nrf2-antibody-91020242063329.jpg)
![Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - Nrf2 antibody [N2C2], Internal detects Nrf2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nrf2 stained by Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-32822_rabbit-polyclonal-nrf2-antibody-9102024202465.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02] - Analysis of monoclonal antibody to NFE2L2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Nrf2-Antibody-2G7-Immunocytochemistry-Immunofluorescence-H00004780-M02-img0004.jpg "Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02] - Analysis of monoclonal antibody to NFE2L2 on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: Nrf2 Antibody (2G7) [H00004780-M02] - Detection limit for recombinant GST tagged NFE2L2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nrf2-Antibody-2G7-ELISA-H00004780-M02-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Nrf2 Antibody (2G7) [H00004780-M02] - EMPA treatment attenuated cardiomyocyte overproduction of reactive oxygen species (ROS) induced by TAC. Cardiac expression of endogenous antioxidant-related genes was significantly reduced after TAC. Representative blot of NRF2 and quantitative results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647080/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/h00004780-m02_mouse-monoclonal-nrf2-antibody-2g7-20920231412572.jpg)
![Western Blot: Nrf2 Antibody (2G7) [H00004780-M02] - EMPA treatment attenuated cardiomyocyte overproduction of reactive oxygen species (ROS) induced by TAC. (A) Left, Mitosox staining of cardiomyocytes in sham, sham + EMPA, TAC & TAC + EMPA groups & Right, quantitative results. (B) Representative blots of NRF-2 & HO-1, & quantitative results. (C) The relative mRNA levels of genes related to endogenous antioxidants. Results are expressed as mean ± SEM, n = 5–7, *p < 0.05 vs. corresponding sham group, †p < 0.05 vs. corresponding TAC vehicle group. One-way ANOVA & Tukey post hoc test. EMPA, empagliflozin; SEM, standard error of the mean; TAC, transverse aortic constriction; NRF-2, the nuclear factor erythroid 2–related factor 2; HO-1, heme oxygenase-1; GCLM, glutamate-cysteine ligase modifier subunit; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004780-m02_mouse-monoclonal-nrf2-antibody-2g7-310202415482245.jpg)
![Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Western-Blot-NBP3-13682-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (HL1021) [NBP3-13682] - Nrf2 antibody [HL1021] detects Nrf2 protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nrf2 stained by Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Immunocytochemistry-Immunofluorescence-NBP3-13682-img0004.jpg)
![Immunohistochemistry-Paraffin: Nrf2 Antibody (HL1021) [NBP3-13682] - Human breast carcinoma. Nrf2 stained by Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Immunohistochemistry-Paraffin-NBP3-13682-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Western-Blot-NBP3-13682-img0001.jpg)
![Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Untreated (-) and treated (+) Rat2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Western-Blot-NBP3-13682-img0002.jpg)
![Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-910202420122022.jpg)
![Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-91020242014615.jpg)
![Immunohistochemistry-Paraffin: Nrf2 Antibody (HL1021) [NBP3-13682] - Nrf2 antibody [HL1021] detects Nrf2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.Nrf2 stained by Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-91020242011413.jpg)
![Western Blot: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] - Western blot analysis of Nrf2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/Nrf2-p-Ser40-Antibody-SU0334-Western-Blot-NBP2-67465-img0009.jpg "Western Blot: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] - Western blot analysis of Nrf2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Flow Cytometry: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -Analysis of RIP3 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67465_rabbit-monoclonal-nrf2-p-ser40-antibody-su0334-flow-cytometry-2572024102417..jpg)
![Immunohistochemistry-Paraffin: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -Rat kidney tissue using anti-RIP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67465_rabbit-monoclonal-nrf2-p-ser40-antibody-su0334-immunohistochemistry-paraffin-2572024102613..jpg)
![Western Blot: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] - Analysis of RIP3 on HT-29 cell lysates with Rabbit anti-RIP3 antibody at 1/1,000 dilution.Lysates/proteins at 10 ug/Lane.Predicted band size: 57 kDaObserved band size: 50 kDaExposure time: 2 minutes;12% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67465_rabbit-monoclonal-nrf2-p-ser40-antibody-su0334-western-blot-2572024102740..jpg)
![Immunohistochemistry: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NBP3-21580.](http://images.novusbio.com/fullsize/nbp3-21580_rabbit-nrf2-p-ser-40-mab-sr2277-5720231721512.jpg "Immunohistochemistry: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NBP3-21580.")
![Western Blot: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Western blot analysis using NBP3-21580 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21580_rabbit-nrf2-p-ser-40-mab-sr2277-67202310627.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Immunofluorescent analysis of HepG2 cells, using NBP3-21580.](http://images.novusbio.com/fullsize/nbp3-21580_rabbit-nrf2-p-ser-40-mab-sr2277-6720231051260.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (1B8) [H00004780-M03] - Analysis of monoclonal antibody to NFE2L2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Nrf2-Antibody-1B8-Immunocytochemistry-Immunofluorescence-H00004780-M03-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (1B8) [H00004780-M03] - Analysis of monoclonal antibody to NFE2L2 on HeLa cell. Antibody concentration 10 ug/ml")
![ELISA: Nrf2 Antibody (1B8) [H00004780-M03] - Detection limit for recombinant GST tagged NFE2L2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nrf2-Antibody-1B8-ELISA-H00004780-M03-img0001.jpg)
![Western Blot-Nrf2 Antibody (1B8) [H00004780-M03] -Detection against Immunogen (36.63 KDa).](http://images.novusbio.com/fullsize/antibody/h00004780-m03_mouse-monoclonal-nrf2-antibody-1b8-western-blot-213202475431.jpg)
![Immunohistochemistry: Nrf2 [p Ser40] Antibody (2E6) [NBP3-26402] - Image of Nrf2 [p Ser40] Antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26402_rabbit-nrf2-p-ser-40-mab-2e6-2722024850464.jpg "Immunohistochemistry: Nrf2 [p Ser40] Antibody (2E6) [NBP3-26402] - Image of Nrf2 [p Ser40] Antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: Nrf2 [p Ser40] Antibody (2E6) [NBP3-26402] - Positive Western Blot detected in HepG2 whole cell lysate, 293 whole cell lysate (treated with Calyculin A or EGF). All lanes: Nrf2 [p Ser40] Antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 KDa Observed band size: 90 KDa](http://images.novusbio.com/fullsize/nbp3-26402_rabbit-nrf2-p-ser-40-mab-2e6-282202411351612.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf2 [p Ser40] Antibody (2E6) [NBP3-26402] - Staining of Hela cells with Nrf2 [p Ser40] Antibody (2E6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26402_rabbit-nrf2-p-ser-40-mab-2e6-282202412475116.jpg)
![Western Blot: Nrf2 Antibody (3G7) [H00004780-M04] - Analysis of NFE2L2 expression in transfected 293T cell line by NFE2L2 monoclonal antibody (M04), clone 3G7. Lane 1: NFE2L2 transfected lysatE (67.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Nrf2-Antibody-3G7-Western-Blot-H00004780-M04-img0003.jpg "Western Blot: Nrf2 Antibody (3G7) [H00004780-M04] - Analysis of NFE2L2 expression in transfected 293T cell line by NFE2L2 monoclonal antibody (M04), clone 3G7. Lane 1: NFE2L2 transfected lysatE (67.8 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Nrf2 Antibody (3G7) [H00004780-M04] - NFE2L2 monoclonal antibody (M04), clone 3G7 Western Blot analysis of NFE2L2 expression in PC-12 ( Cat # L012V1 ).](http://images.novusbio.com/fullsize/Nrf2-Antibody-3G7-Western-Blot-H00004780-M04-img0002.jpg)
![Western Blot: Nrf2 Antibody (JE30-14) [NBP3-32661] - Western blot analysis of Nrf2 on different lysates with Rabbit anti-Nrf2 antibody (NBP3-32661) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 2uM MG-132 for 18 hours whole cell lysate Lane 3: HCT 116 whole cell lysate Lane 4: HCT 116 treated with 25uM MG-132 for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 68 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32661) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32661_rabbit-nrf2-mab-je30-14-27820241631686.jpg "Western Blot: Nrf2 Antibody (JE30-14) [NBP3-32661] - Western blot analysis of Nrf2 on different lysates with Rabbit anti-Nrf2 antibody (NBP3-32661) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 2uM MG-132 for 18 hours whole cell lysate Lane 3: HCT 116 whole cell lysate Lane 4: HCT 116 treated with 25uM MG-132 for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 68 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32661) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2D12) [NBP3-26113] - Staining of Hela Cells with Nrf2 Antibody (2D12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26113_rabbit-nrf2-mab-2d12-282202412493615.jpg "Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2D12) [NBP3-26113] - Staining of Hela Cells with Nrf2 Antibody (2D12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")