Myosin Phosphatase Antibodies
We offer Myosin Phosphatase Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each Myosin Phosphatase Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Myosin Phosphatase Antibodies can be used in a variety of model species: Chicken, Human, Mouse, Rat. Use the list below to choose the Myosin Phosphatase Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Myosin Phosphatase polyclonal antibodies and browse our Myosin Phosphatase monoclonal antibody catalog.
Alternate Names for Myosin Phosphatase Antibodies
anti-Myosin Phosphatase antibody, anti-PPP1R12A antibody, anti-MBSmyosin phosphatase, target subunit 1 antibody, anti-MGC133042 antibody, anti-Myosin phosphatase target subunit 1 antibody, anti-Myosin phosphatase-targeting subunit 1 antibody, anti-MYPT1protein phosphatase 1 regulatory subunit 12A antibody, anti-protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody, anti-Protein phosphatase myosin-binding subunit antibody
11 Results for "myosin-phosphatase" in Primary Antibodies
![Immunoprecipitation: Myosin Phosphatase Antibody [NBP3-35077] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug Myosin Phosphatase antibody. Western blot was performed from the immunoprecipitate using Myosin Phosphatase antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415161825.jpg "Immunoprecipitation: Myosin Phosphatase Antibody [NBP3-35077] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug Myosin Phosphatase antibody. Western blot was performed from the immunoprecipitate using Myosin Phosphatase antibody at a dilution of 1:1000.")
![Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP3-35077] - Immunofluorescence analysis of HeLa cells using Myosin Phosphatase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415152878.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP3-35077] - Immunofluorescence analysis of PC-12 cells using Myosin Phosphatase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415175270.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP3-35077] - Immunofluorescence analysis of NIH/3T3 cells using Myosin Phosphatase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-91220241581217.jpg)
![Western Blot: Myosin Phosphatase Antibody [NBP3-35077] - Western blot analysis of various lysates using Myosin Phosphatase Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415133450.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP3-35077] - Immunofluorescence analysis of U2OS cells using Myosin Phosphatase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415111826.jpg)
![Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody NB110-38889 (lot NB110-38889-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot NB110-38889-1) of this antibody and by another rabbit anti-MYPT1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Western-Blot-NB110-38889-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody NB110-38889 (lot NB110-38889-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot NB110-38889-1) of this antibody and by another rabbit anti-MYPT1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - (4ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455812.jpg "Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - (4ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-13112023114762.jpg)
![Flow Cytometry: Myosin Phosphatase Antibody [NBP2-75097] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455813.jpg)
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455817.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455810.jpg)
![Western Blot: Myosin Phosphatase Antibody [NBP2-68929] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Western-Blot-NBP2-68929-img0001.jpg "Western Blot: Myosin Phosphatase Antibody [NBP2-68929] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Stainng of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-68929-img0006.jpg)
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-68929-img0003.jpg)
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-68929-img0004.jpg)
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glanular and smooth muscle cells.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-68929-img0005.jpg)
![Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: untreated C2C12 lysate| Lane 2: pervanadate treated C2C12 lysate. Resolved by electrophoresis, transferred to nitrocellulose and probed with Myosin Phosphatase [p Thr853] antibody (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates phosphorylated MYPT1 substrate (80kD).](http://images.novusbio.com/fullsize/Myosin-Phosphatase-[p-Thr853]-Antibody-NBP2-13185-img0001.jpg "Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: untreated C2C12 lysate| Lane 2: pervanadate treated C2C12 lysate. Resolved by electrophoresis, transferred to nitrocellulose and probed with Myosin Phosphatase [p Thr853] antibody (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates phosphorylated MYPT1 substrate (80kD).")
![Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: ROKa/ROCK-II only Lane 2: MYPT1 substrate only Lane 3: MYPT1 substrate posphorylated by ROKa/ROCK-II 3](http://images.novusbio.com/fullsize/Myosin-Phosphatase-[p-Thr853]-Antibody-NBP2-13185-img0002.jpg)
![Western Blot: Myosin Phosphatase [p Thr853] Antibody (SA19) [NBP2-29680] - Untreated HEK293 cell lysate (lane 1) and HEK293 cell lysate phosphorylated by ROKalpha /ROCKII (lane 2) were probed with anti-phospho-MyPT1 (Thr850), clone SA19 (1:1000 dilution).](http://images.novusbio.com/fullsize/Myosin-Phosphatase-p-Thr853-Antibody-SA19-Western-Blot-NBP2-29680-img0001.jpg "Western Blot: Myosin Phosphatase [p Thr853] Antibody (SA19) [NBP2-29680] - Untreated HEK293 cell lysate (lane 1) and HEK293 cell lysate phosphorylated by ROKalpha /ROCKII (lane 2) were probed with anti-phospho-MyPT1 (Thr850), clone SA19 (1:1000 dilution).")
![Western Blot: Myosin Phosphatase [p Ser668] Antibody [NBP3-38642] - Western blot analysis of various lysates using Myosin Phosphatase Rabbit pAb at 1:2000 dilution. Both HeLa cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38642_rabbit-myosin-phosphatase-p-ser-668-pab-1012202411392921.jpg "Western Blot: Myosin Phosphatase [p Ser668] Antibody [NBP3-38642] - Western blot analysis of various lysates using Myosin Phosphatase Rabbit pAb at 1:2000 dilution. Both HeLa cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: Myosin Phosphatase [p Thr853] Antibody [NBP3-38643] - Western blot analysis of various lysates using Myosin Phosphatase Rabbit pAb ( AP0916) at 1:1000 dilution. Hela cells were treated by CIP(20uL/400ul) at 37C for 1 hour. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38643_rabbit-myosin-phosphatase-p-thr-853-pab-9122024159566.jpg "Western Blot: Myosin Phosphatase [p Thr853] Antibody [NBP3-38643] - Western blot analysis of various lysates using Myosin Phosphatase Rabbit pAb ( AP0916) at 1:1000 dilution. Hela cells were treated by CIP(20uL/400ul) at 37C for 1 hour. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-58950] - Staining of human cell line U-2 OS shows localization to cytosol & actin filaments.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58950-img0001.jpg "Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-58950] - Staining of human cell line U-2 OS shows localization to cytosol & actin filaments.")
![Western Blot: Myosin Phosphatase Antibody [NBP3-16931] - Western blot analysis of extracts of Jurkat cells, using Myosin Phosphatase Rabbit pAb (NBP3-16931) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Western-Blot-NBP3-16931-img0001.jpg "Western Blot: Myosin Phosphatase Antibody [NBP3-16931] - Western blot analysis of extracts of Jurkat cells, using Myosin Phosphatase Rabbit pAb (NBP3-16931) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Myosin Phosphatase [p Ser507] Antibody [NBP3-38639] - Western blot analysis of various lysates using Myosin Phosphatase Rabbit pAb at 1:2000 dilution or PPP1R12A antibody. HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38639_rabbit-myosin-phosphatase-p-ser-507-pab-1012202411555910.jpg "Western Blot: Myosin Phosphatase [p Ser507] Antibody [NBP3-38639] - Western blot analysis of various lysates using Myosin Phosphatase Rabbit pAb at 1:2000 dilution or PPP1R12A antibody. HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.")