Mitofilin Antibodies
We offer Mitofilin Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each Mitofilin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Mitofilin Antibodies can be used in a variety of model species: Canine, Feline, Human, Mouse, Rat. Use the list below to choose the Mitofilin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Mitofilin polyclonal antibodies and browse our Mitofilin monoclonal antibody catalog.
Alternate Names for Mitofilin Antibodies
anti-Mitofilin antibody, anti-IMMT antibody, anti-DKFZp779P1653 antibody, anti-inner membrane protein, mitochondrial antibody, anti-MGC111146 antibody, anti-Mic 60 antibody, anti-Mic60 antibody, anti-MICOS Complex Subunit MIC60 antibody, anti-mitochondrial (mitofilin) antibody, anti-motor protein antibody, anti-p87/89 antibody, anti-proliferation-inducing gene 4 antibody
12 Results for "mitofilin" in Primary Antibodies
![Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0006.jpg "Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.")
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NB100-1919] - HeLa cells with antibody 1:500 dilution labeling mitochondria.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1919-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. The representative image shows a specific and expected granular staining of Mitofilin in the keratinocytes as well as the other cells of epidermal layer with no staining in basal cells. [60X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0009.jpg)
![Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import-defective. HEK293 cells that transiently expressed wild type or mutant COA7HIS were solubilized, and the affinity purification of COA7HIS was performed. The samples were analyzed by reducing and non-reducing SDS-PAGE and Western blot. Load: 3%. Eluate: 100%. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201809561), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0012.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P analysis of Mitofilin/Mitochondrial inner membrane protein in a transverse section of normal human skeletal muscle using Mitofilin antibody at 1:200 dilution. The myocytes showed an expected and very specific granular staining in the cytoplasm. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0011.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. Specific cytoplasmic positivity of Mitofilin was observed in the keratinocytes as well as other cells of the epidermal layer except for basal cells and dermal connective tissue. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0010.jpg)
![Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import‐defectiveASchematic representation of wild‐type & mutant COA7.BCellular protein extracts were isolated from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The samples were analyzed by reducing SDS–PAGE & Western blot.CCellular fractions were prepared from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The fractions were analyzed by reducing SDS–PAGE & Western blot. T, total; C, cytosol; M, mitochondria.DHEK293 cells that transiently expressed wild‐type or mutant COA7HIS were solubilized, & the affinity purification of COA7HIS was performed. The samples were analyzed by reducing & non‐reducing SDS–PAGE & Western blot. Load: 3%. Eluate: 100%.EEqual amounts of wild‐type & mutant radiolabeled [35S]COA7 precursors were imported into mitochondria isolated from HEK293 cells. The samples were analyzed by reducing SDS–PAGE & autoradiography. The results of three biological replicates were analyzed, quantified, & normalized to wild‐type COA7 at 45 min. The data are expressed as a mean ± SEM (n = 3). IAA, iodoacetamide.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30885959), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1919_rabbit-polyclonal-mitofilin-antibody-310202415532097.jpg)
![Western Blot: Mitofilin Antibody [NBP2-38286] - Analysis using Anti-IMMT antibody NBP2-38286 (A) shows similar pattern to independent antibody NBP2-38285 (B).](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP2-38286-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Mitofilin Antibody [NBP2-38286] - Analysis using Anti-IMMT antibody NBP2-38286 (A) shows similar pattern to independent antibody NBP2-38285 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NBP2-38286] - Staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38286-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38286] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38286-img0012.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38286] - Staining of human heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38286-img0009.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38286] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38286-img0010.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38286] - Staining of human stomach shows strong grtanular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38286-img0011.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining in human parathyroid gland and pancreas tissues using anti-IMMT antibody. Corresponding IMMT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining in human parathyroid gland and pancreas tissues using anti-IMMT antibody. Corresponding IMMT RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Mitofilin Antibody [NBP2-38285] - Analysis using Anti-IMMT antibody NBP2-38285 (A) shows similar pattern to independent antibody NBP2-38286 (B).](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP2-38285-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NBP2-38285] - Immunofluorescent staining of human cell line HEK 293 shows localization to mitochondria.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38285-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0005.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0006.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414304127.jpg "Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32597) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414333672.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-29202410334014.jpg)
![Western Blot: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/100,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Saos-2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-3920248485018.jpg)
![Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-27820241633128.jpg "Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32596) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-298202413585565.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32596, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29820241430416.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29202410334040.jpg)
![Western Blot: Mitofilin Antibody [NBP3-03346] - Analysis of extracts of Mouse brain, using IMMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP3-03346-img0003.jpg "Western Blot: Mitofilin Antibody [NBP3-03346] - Analysis of extracts of Mouse brain, using IMMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: Mitofilin Antibody [NBP3-03346] - Analysis of extracts of various cell lines, using IMMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP3-03346-img0002.jpg)
![Immunohistochemistry: Mitofilin Antibody - BSA Free [NBP3-03346] - Immunohistochemistry analysis of Mitofilin in paraffin-embedded rat heart tissue using Mitofilin Rabbit pAb (A2751) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03346_rabbit-polyclonal-mitofilin-antibody-52202519302993.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody - BSA Free [NBP3-03346] - Confocal immunofluorescence analysis of U2OS cells using Mitofilin Rabbit pAb (A2751) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03346_rabbit-polyclonal-mitofilin-antibody-52202519302926.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/antibody/nbp3-23409_mouse-mitofilin-mab-m027-207202313321516.png "Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23409_mouse-mitofilin-mab-m027-1992023199280.jpg)
![Western Blot: Mitofilin Antibody (M027) [NBP3-23409] - Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (NBP3-23409) at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23409_mouse-mitofilin-mab-m027-19920231820291.jpg)
![Western Blot: Mitofilin Antibody [NBP3-03850] - Analysis of extracts of various cell lines, using Mitofilin antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Ba](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP3-03850-img0001.jpg "Western Blot: Mitofilin Antibody [NBP3-03850] - Analysis of extracts of various cell lines, using Mitofilin antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Ba")
![Immunoprecipitation:Mitofilin AntibodyNBP3-30256] -Detection of human IMMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IMMT antibody NBP3-30256 (lot NBP3-30256-1) used for IP at 6 µg per reaction. IMMT was also immunoprecipitated by rabbit anti-IMMT.. For blotting immunoprecipitated IMMT, NBP3-30256 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30256_rabbit-mitofilin-pab-301220241524147.jpg "Immunoprecipitation:Mitofilin AntibodyNBP3-30256] -Detection of human IMMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IMMT antibody NBP3-30256 (lot NBP3-30256-1) used for IP at 6 µg per reaction. IMMT was also immunoprecipitated by rabbit anti-IMMT.. For blotting immunoprecipitated IMMT, NBP3-30256 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot:[NBP3-29603]-Detection of human IMMT by western blot. Samples: Whole cell lysate from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IMMT antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29603_rabbit-mitofilin-pab-231202512101653.jpg "Western Blot:[NBP3-29603]-Detection of human IMMT by western blot. Samples: Whole cell lysate from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IMMT antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:Mitofilin AntibodyNBP3-29812] -Detection of human IMMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IMMT antibody NBP3-29812 (lot NBP3-29812-1) used for IP at 6 µg per reaction. IMMT was also immunoprecipitated by rabbit anti-IMMT.. For blotting immunoprecipitated IMMT, NBP3-29812 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29812_rabbit-mitofilin-pab-30122024188590.jpg "Immunoprecipitation:Mitofilin AntibodyNBP3-29812] -Detection of human IMMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IMMT antibody NBP3-29812 (lot NBP3-29812-1) used for IP at 6 µg per reaction. IMMT was also immunoprecipitated by rabbit anti-IMMT.. For blotting immunoprecipitated IMMT, NBP3-29812 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Mitofilin Antibody (S03-6H5) [NBP3-15060] - Western blot detection of Mitofilin in K562, Molt-4, Raji cell lysates using Mitofilin Rabbit mAb (1:500 diluted). Predicted band size: 84kDa. Observed band size: 84kDa.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-S03-6H5-Western-Blot-NBP3-15060-img0001.jpg "Western Blot: Mitofilin Antibody (S03-6H5) [NBP3-15060] - Western blot detection of Mitofilin in K562, Molt-4, Raji cell lysates using Mitofilin Rabbit mAb (1:500 diluted). Predicted band size: 84kDa. Observed band size: 84kDa.")