IQGAP1 Antibodies
We offer IQGAP1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each IQGAP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our IQGAP1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the IQGAP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our IQGAP1 polyclonal antibodies and browse our IQGAP1 monoclonal antibody catalog.
Alternate Names for IQGAP1 Antibodies
anti-IQGAP1 antibody, anti-HUMORFA01 antibody, anti-IQ motif containing GTPase activating protein 1 antibody, anti-KIAA0051ras GTPase-activating-like protein IQGAP1 antibody, anti-p195RasGAP-like with IQ motifs antibody, anti-SAR1 antibody
9 Results for "iqgap1" in Primary Antibodies
![Knockdown Validated: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 mRNA and protein levels in HO-8910PM-shIQGAP1, HO-8910PM-shRNA negative and un-transfected HO-8910PM cells were determined by RT-PCR and Western blot analysis, respectively. Image collected and cropped by CiteAb from the following publication (jeccr.biomedcentral.com/articles/10.1186/1756-9966-27-77), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Knockdown-Validated-H00008826-M01-img0011.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 mRNA and protein levels in HO-8910PM-shIQGAP1, HO-8910PM-shRNA negative and un-transfected HO-8910PM cells were determined by RT-PCR and Western blot analysis, respectively. Image collected and cropped by CiteAb from the following publication (jeccr.biomedcentral.com/articles/10.1186/1756-9966-27-77), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - Western blot analysis for IQGAP1 in whole-cell lysates from the indicated cell lines. Image collected and cropped by CiteAb from the following publication (jeccr.biomedcentral.com/articles/10.1186/1756-9966-27-77), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Western-Blot-H00008826-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: IQGAP1 Antibody (2C5) [H00008826-M01] - Analysis of monoclonal antibody to IQGAP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Immunocytochemistry-Immunofluorescence-H00008826-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: IQGAP1 Antibody (2C5) [H00008826-M01] - Analysis of monoclonal antibody to IQGAP1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Immunohistochemistry-Paraffin-H00008826-M01-img0008.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 monoclonal antibody (M01), clone 2C5 Analysis of IQGAP1 expression in C32.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Western-Blot-H00008826-M01-img0010.jpg)
![Sandwich ELISA: IQGAP1 Antibody (2C5) [H00008826-M01] - Detection limit for recombinant GST tagged IQGAP1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Sandwich-ELISA-H00008826-M01-img0009.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - indicates that IQGAP1 mRNA & protein levels in HO-8910PM-shIQGAP1, HO-8910PM-shRNA negative & un-transfected HO-8910PM cells were determined by RT-PCR & Western blot analysis, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19036171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-310202415345311.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - shows that IQGAP1 expression & cell invasive phenotype in ovarian cancer cell lines. (a) RT-PCR analysis for IQGAP1 using total RNA form HO-8910, SK-OV-3 & HO-8910PM cells. (b) Western blot analysis for IQGAP1 in whole-cell lysates from the indicated cell lines. (c) High IQGAP1 expression associated with enhanced invasive potential of ovarian cancer cells. HO-8910, SK-OV-3 & HO-8910PM cells were seeded onto a Matrigel-coated invasion chamber & the number of invading cells was determined as described before. *, P < 0.05 versus SK-OV-3 or HO-8910 cells. (d) High IQGAP1 expression correlated with enhanced migratory potential of ovarian cancer cells. HO-8910, SK-OV-3 & HO-8910PM cells were seeded onto a Boyden chamber & the number of migrating cells was determined as described before. *, P < 0.05 versus SK-OV-3 or HO-8910 cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19036171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-310202415483611.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 induces the epithelial-to-mesenchymal transition, invasiveness, & proliferation of endometrial cancer (EC) cellsA. Reverse transcription quantitative PCR (qPCR) analysis of IQGAP1 mRNA in the immortalized human endometrial cell line EM & the EC cells HEC-1, HEC-50, & HEC-50-HI (HI). The results are presented as the fold-change in expression compared to the EM cells. B. Western blot analysis of the IQGAP1 protein in EC cells. C, E. The expression of IQGAP1, E-cadherin, & N-cadherin proteins in HI cells transfected with control (Ctr) or IQGAP1 siRNA (C) & in HEC-1 cells expressing either the control or IQGAP1 vector (E). D, F. Phase-contrast microscopy shows the morphology of HI cells transfected with control or IQGAP1 siRNA (D) & HEC-1 cells transfected with the control or IQGAP1 vector (F). G-I. Detection of migration (G), invasion (H), & proliferation (I) in HI & HEC-1 cells after the indicated transfection. J, K. qPCR analysis of ZO-1, CK-18, & Vimentin expression in HI (J) & HEC-1 (K) cells, transfected as indicated. L. Representative images from the invasion assays. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-310202415482215.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 induces the epithelial-to-mesenchymal transition, invasiveness, & proliferation of endometrial cancer (EC) cellsA. Reverse transcription quantitative PCR (qPCR) analysis of IQGAP1 mRNA in the immortalized human endometrial cell line EM & the EC cells HEC-1, HEC-50, & HEC-50-HI (HI). The results are presented as the fold-change in expression compared to the EM cells. B. Western blot analysis of the IQGAP1 protein in EC cells. C, E. The expression of IQGAP1, E-cadherin, & N-cadherin proteins in HI cells transfected with control (Ctr) or IQGAP1 siRNA (C) & in HEC-1 cells expressing either the control or IQGAP1 vector (E). D, F. Phase-contrast microscopy shows the morphology of HI cells transfected with control or IQGAP1 siRNA (D) & HEC-1 cells transfected with the control or IQGAP1 vector (F). G-I. Detection of migration (G), invasion (H), & proliferation (I) in HI & HEC-1 cells after the indicated transfection. J, K. qPCR analysis of ZO-1, CK-18, & Vimentin expression in HI (J) & HEC-1 (K) cells, transfected as indicated. L. Representative images from the invasion assays. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-31020241548516.jpg)
![Western Blot: IQGAP1 Antibody [NBP1-06529] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IQGAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Western-Blot-NBP1-06529-img0011.jpg "Western Blot: IQGAP1 Antibody [NBP1-06529] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IQGAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-06529] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-IQGAP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-06529-img0009.jpg)
![Immunoprecipitation: IQGAP1 Antibody [NBP1-06529] - Detection of human IQGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IQGAP1 antibody NBP1-06529 (lot NBP1-06529-2) used for IP at 3 ug per reaction. IQGAP1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06529-1). For blotting immunoprecipitated IQGAP1, NBP1-06529 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunoprecipitation-NBP1-06529-img0010.jpg)
![Western Blot: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Western blot analysis of IQGAP1 on different lysates with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 189 kDa Observed band size: 189 kDa Exposure time: 1 minute 40 second; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32479) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32479_rabbit-iqgap1-mab-je50-66-278202415295553.jpg "Western Blot: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Western blot analysis of IQGAP1 on different lysates with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 189 kDa Observed band size: 189 kDa Exposure time: 1 minute 40 second; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32479) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32479) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32479_rabbit-iqgap1-mab-je50-66-29820241492650.jpg)
![Immunocytochemistry/ Immunofluorescence: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Immunocytochemistry analysis of HeLa cells labeling IQGAP1 with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32479_rabbit-iqgap1-mab-je50-66-29202410484868.jpg)
![Flow Cytometry: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Flow cytometric analysis of HeLa cells labeling IQGAP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32479, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32479_rabbit-iqgap1-mab-je50-66-29202416372144.jpg)
![Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Western-Blot-NBP1-83143-img0008.jpg "Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining in human endometrium and skeletal muscle tissues using anti-IQGAP1 antibody. Corresponding IQGAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83143-img0014.jpg)
![Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Western-Blot-NBP1-83143-img0007.jpg)
![Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining of human endometrium shows high expression.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83143-img0012.jpg)
![Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83143-img0013.jpg)
![Western Blot: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Western blot analysis of extracts of various cell lines, using IQGAP1 Rabbit mAb (NBP3-16879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-7I6X1-Western-Blot-NBP3-16879-img0002.jpg "Western Blot: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Western blot analysis of extracts of various cell lines, using IQGAP1 Rabbit mAb (NBP3-16879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Immunohistochemistry of paraffin-embedded human liver using IQGAP1 Rabbit mAb (NBP3-16879) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-7I6X1-Immunohistochemistry-Paraffin-NBP3-16879-img0001.jpg)
![Western Blot: IQGAP1 Antibody [NBP1-51980] - (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Western-Blot-NBP1-51980-img0002.jpg "Western Blot: IQGAP1 Antibody [NBP1-51980] - (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
