HUWE1 Antibodies
We offer HUWE1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Western Blot. Each HUWE1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HUWE1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the HUWE1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HUWE1 polyclonal antibodies and browse our HUWE1 monoclonal antibody catalog.
Alternate Names for HUWE1 Antibodies
anti-HUWE1 antibody, anti-EC 6.3.2 antibody, anti-EC 6.3.2.6 antibody, anti-HECT, UBA and WWE domain containing 1 antibody, anti-HECTH9 antibody, anti-HSPC272 antibody, anti-KIAA0312MULE antibody, anti-Mcl-1 ubiquitin ligase E3 antibody, anti-Mule antibody, anti-URE-binding protein 1 antibody, anti-UREB1UBA and WWE domain-containing protein 1 antibody
7 Results for "huwe1" in Primary Antibodies
![Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3seconds.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Western-Blot-NB100-652-img0011.jpg "Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3seconds.")
![Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiLasu1/Ureb1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Immunohistochemistry-Paraffin-NB100-652-img0012.jpg)
![Immunoprecipitation: HUWE1 Antibody [NB100-652] - Detection of human Lasu1/Ureb1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Lasu1/Ureb1 antibody NB100-652 (lot NB100-652-9) used for IP at 6 ug per reaction. Lasu1/Ureb1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-652-8). For blotting immunoprecipitated Lasu1/Ureb1, NB100-652 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Immunoprecipitation-NB100-652-img0013.jpg)
![Western Blot: HUWE1 Antibody (BLR129H) [NBP3-14712] - Rabbit antiLasu1/Ureb1 recombinant monoclonal antibody [BLR129H] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/HUWE1-Antibody-BLR129H-Western-Blot-NBP3-14712-img0004.jpg "Western Blot: HUWE1 Antibody (BLR129H) [NBP3-14712] - Rabbit antiLasu1/Ureb1 recombinant monoclonal antibody [BLR129H] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: HUWE1 Antibody (BLR129H) [NBP3-14712] - SiHa cells by immunocytochemistry. Antibody: Rabbit anti-Lasu1/Ureb1recombinant monoclonal antibody [BLR129H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB](http://images.novusbio.com/fullsize/HUWE1-Antibody-BLR129H-Immunocytochemistry-Immunofluorescence-NBP3-14712-img0005.jpg)
![Immunohistochemistry-Paraffin: HUWE1 Antibody (BLR129H) [NBP3-14712] - Head & neck SCC. Rabbit antiLasu1/Ureb1 recombinant monoclonal antibody [BLR129H]. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/HUWE1-Antibody-BLR129H-Immunohistochemistry-Paraffin-NBP3-14712-img0003.jpg)
![Immunoprecipitation: HUWE1 Antibody [NBP3-14712] - : Rabbit anti-Lasu1/Ureb1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. Lasu1/Ureb1 was alsoimmunoprecipitated by other Lasu1/Ureb1 antibodies. For blotting immunoprecipitatedLasu1-Ureb1, was used at 1:1000.Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Immunoprecipitation-NBP3-14712-img0001.jpg)
![Western Blot: HUWE1 Antibody (PSH02-38) [NBP3-32455] - Western blot analysis of HUWE1 on PC-12 cell lysates with Rabbit anti-HUWE1 antibody (NBP3-32455) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 482.7 kDa Observed band size: 482.7 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32455) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32455_rabbit-huwe1-mab-psh02-38-278202416285293.jpg "Western Blot: HUWE1 Antibody (PSH02-38) [NBP3-32455] - Western blot analysis of HUWE1 on PC-12 cell lysates with Rabbit anti-HUWE1 antibody (NBP3-32455) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 482.7 kDa Observed band size: 482.7 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32455) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HUWE1 Antibody (PSH02-38) [NBP3-32455] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-HUWE1 antibody (NBP3-32455) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32455_rabbit-huwe1-mab-psh02-38-298202414304148.jpg)
![Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody (PSH02-38) [NBP3-32455] - Immunocytochemistry analysis of HeLa cells labeling HUWE1 with Rabbit anti-HUWE1 antibody (NBP3-32455) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HUWE1 antibody (NBP3-32455) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32455_rabbit-huwe1-mab-psh02-38-29202410334072.jpg)
![Flow Cytometry: HUWE1 Antibody (PSH02-38) [NBP3-32455] - Flow cytometric analysis of HeLa cells labeling HUWE1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32455, 1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32455_rabbit-huwe1-mab-psh02-38-29202416372110.jpg)
![Western Blot: HUWE1 Antibody [NBP1-76795] - Daudi cell lysate with HUWE1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Western-Blot-NBP1-76795-img0003.jpg "Western Blot: HUWE1 Antibody [NBP1-76795] - Daudi cell lysate with HUWE1 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody - BSA Free [NBP1-76795] - Immunofluorescence of HUWE1 in Daudi cells with HUWE1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76795_rabbit-polyclonal-huwe1-antibody-271120242092032.jpg)
![Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody - BSA Free [NBP1-76795] - Immunocytochemistry of HUWE1 in Daudi cells with HUWE1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76795_rabbit-polyclonal-huwe1-antibody-271120242032482.jpg)
![Immunocytochemistry/Immunofluorescence: HUWE1 Antibody [NBP1-83127] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83127-img0004.jpg "Immunocytochemistry/Immunofluorescence: HUWE1 Antibody [NBP1-83127] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: HUWE1 Antibody [NBP1-83127] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Immunohistochemistry-Paraffin-NBP1-83127-img0002.jpg)
![Western Blot:[NBP3-30079]-Detection of human Lasu1/Ureb1 by western blot. Samples: Whole cell lysate from HEK293T and HeLa cells. Antibodies: Affinity purified goat anti-Lasu1/Ureb1 antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30079_goat-huwe1-pab-23120251204034.jpg "Western Blot:[NBP3-30079]-Detection of human Lasu1/Ureb1 by western blot. Samples: Whole cell lysate from HEK293T and HeLa cells. Antibodies: Affinity purified goat anti-Lasu1/Ureb1 antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry:[NBP3-29248]-Detection of human Lasu1/Ureb1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1.](http://images.novusbio.com/fullsize/nbp3-29248_rabbit-huwe1-pab-231202512101659.jpg "Immunohistochemistry:[NBP3-29248]-Detection of human Lasu1/Ureb1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1.")