| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1054AF405 | |||
| Alexa Fluor 488 | NB21-1054AF488 | |||
| Alexa Fluor 647 | NB21-1054AF647 | |||
| Alexa Fluor 700 | NB21-1054AF700 | |||
| Biotin | NB21-1054B | |||
| DyLight 405 | NB21-1054V | |||
| DyLight 488 | NB21-1054G | |||
| DyLight 550 | NB21-1054R | |||
| DyLight 650 | NB21-1054C | |||
| FITC | NB21-1054F | |||
| HRP | NB21-1054H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1054SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1053AF405 | |||
| Alexa Fluor 488 | NB21-1053AF488 | |||
| Alexa Fluor 647 | NB21-1053AF647 | |||
| Alexa Fluor 700 | NB21-1053AF700 | |||
| Biotin | NB21-1053B | |||
| DyLight 405 | NB21-1053V | |||
| DyLight 488 | NB21-1053G | |||
| DyLight 550 | NB21-1053R | |||
| DyLight 650 | NB21-1053C | |||
| FITC | NB21-1053F | |||
| HRP | NB21-1053H | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1051AF405 | |||
| Alexa Fluor 488 | NB21-1051AF488 | |||
| Alexa Fluor 647 | NB21-1051AF647 | |||
| Alexa Fluor 700 | NB21-1051AF700 | |||
| Biotin | NB21-1051B | |||
| DyLight 405 | NB21-1051V | |||
| DyLight 488 | NB21-1051G | |||
| DyLight 550 | NB21-1051R | |||
| DyLight 650 | NB21-1051C | |||
| FITC | NB21-1051F | |||
| HRP | NB21-1051H | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1101AF405 | |||
| Alexa Fluor 488 | NB21-1101AF488 | |||
| Alexa Fluor 647 | NB21-1101AF647 | |||
| Alexa Fluor 700 | NB21-1101AF700 | |||
| Biotin | NB21-1101B | |||
| DyLight 405 | NB21-1101V | |||
| DyLight 488 | NB21-1101G | |||
| DyLight 550 | NB21-1101R | |||
| DyLight 650 | NB21-1101C | |||
| FITC | NB21-1101F | |||
| HRP | NB21-1101H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1101SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26019 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26021 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26020 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9448-SP | |||
| Carrier Free | IC9448V-100UG | |||
| Carrier Free | IC9448T-100UG | |||
| Carrier Free | IC9448S-100UG | |||
| Carrier Free | IC9448R-100UG | |||
| Carrier Free | IC9448N-100UG | |||
| Carrier Free | IC9448G-100UG | |||
| Carrier Free | IC9448U-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52482-0.025mg | |||
![Dot Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Dot-Blot-NB21-1054-img0010.jpg "Dot Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1054 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1054-img0013.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Western-Blot-NB21-1054-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Histone H3.2 K9me3 pT6 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1054-img0016.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9Me3/pT6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Western-Blot-NB21-1054-img0012.jpg)
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - 2 ug of NB21-1053 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Dimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1053-img0008.jpg "Chromatin Immunoprecipitation: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - 2 ug of NB21-1053 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Western Blot: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - Analysis of H3K9me2/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9-p-Thr6]-Antibody-Western-Blot-NB21-1053-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - Histone H3.2 K9me2 pT6 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Dimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1053-img0007.jpg)
![Dot Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Analysis of Histone H3 [phospho T6] using the peptides stated above in 1.0, 0.3, 0.1 and 0.03 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6]-Antibody-Dot-Blot-NB21-1051-img0008.jpg "Dot Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Analysis of Histone H3 [phospho T6] using the peptides stated above in 1.0, 0.3, 0.1 and 0.03 picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6] Antibody [NB21-1051] - Staining of Histone H3 [phospho T6] in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1051-img0013.jpg)
![Western Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Analysis of H3pt6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6]-Antibody-Western-Blot-NB21-1051-img0010.jpg)
![Western Blot: Histone H3 [p Thr6] Antibody [NB21-1051] - Detection of Histone H3 [phospho T6] in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6]-Antibody-Western-Blot-NB21-1051-img0009.jpg)
![Dot Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 with the peptides stated above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Dot-Blot-NB21-1101-img0003.jpg "Dot Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 with the peptides stated above in picomoles of peptide.")
![Western Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Western-Blot-NB21-1101-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr11] Antibody [NB21-1101] - Staining of H3pT11 in HeLa cells using FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1101-img0006.jpg)
![Western Blot: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3). Using NBP2-61546, showed a band of Histone H3 phosphorylated at threonine 3 in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-p-Thr3-Antibody-RM159-Western-Blot-NBP2-61546-img0007.jpg "Western Blot: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3). Using NBP2-61546, showed a band of Histone H3 phosphorylated at threonine 3 in HeLa cells. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunocytochemistry of HeLa cells using NBP2-61546 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-p-Thr3-Antibody-RM159-Immunocytochemistry-Immunofluorescence-NBP2-61546-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunocytochemistry of HeLa cells using NBP2-61546 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-p-Thr3-Antibody-RM159-Immunocytochemistry-Immunofluorescence-NBP2-61546-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using NBP2-61546 at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-61546_rabbit-monoclonal-histone-h3-p-thr3-antibody-rm159-131020231935490.png)
![Western Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - Western blot was performed on whole cell extracts from untreated HeLa cells (25 ug, lane 1), on histone extracts from HeLa cells treated with colcemid (15 ug, lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3T11p . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Western-Blot-NBP2-59274-img0001.jpg "Western Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - Western blot was performed on whole cell extracts from untreated HeLa cells (25 ug, lane 1), on histone extracts from HeLa cells treated with colcemid (15 ug, lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3T11p . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr11] Antibody [NBP2-59274] - ChIP assays were performed using human HeLa cells, treated with colcemid, the antibody against H3T11p and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the coding region of the inactive MYT1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Chromatin-Immunoprecipitation-NBP2-59274-img0004.jpg)
![Dot Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - To test the cross reactivity of the antibody against H3T11p, a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H3T11. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Dot-Blot-NBP2-59274-img0002.jpg)
![ELISA: Histone H3 [p Thr11] Antibody [NBP2-59274] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3T11p in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,700.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-ELISA-NBP2-59274-img0003.jpg)
![Immunofluorescence: Histone H3 [p Thr11] Antibody [NBP2-59274] - HeLa cells were treated with colcemid and stained with the antibody against H3T11p and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3T11p antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Immunofluorescence-NBP2-59274-img0005.jpg)
![Western Blot: Histone H3 [p Thr11] Antibody (RM164) [NBP2-61521] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61521 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-p-Thr11-Antibody-RM164-Western-Blot-NBP2-61521-img0002.jpg "Western Blot: Histone H3 [p Thr11] Antibody (RM164) [NBP2-61521] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61521 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.")
![Western Blot: Histone H3 [p Thr6] Antibody (RM160) [NBP2-61520] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61520 at 0.1 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-p-Thr6-Antibody-RM160-Western-Blot-NBP2-61520-img0002.jpg "Western Blot: Histone H3 [p Thr6] Antibody (RM160) [NBP2-61520] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61520 at 0.1 ug/ml. Observed molecular weight is ~17 kDa.")
![ELISA: Histone H3 [p Thr45] Antibody [NBP2-62795] - To determine the titer of the antibody, analysis was performed using a serial dilution of the Diagenode antibody directed against H3T45p in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:350,000.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr45]-Antibody-ELISA-NBP2-62795-img0001.jpg "ELISA: Histone H3 [p Thr45] Antibody [NBP2-62795] - To determine the titer of the antibody, analysis was performed using a serial dilution of the Diagenode antibody directed against H3T45p in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:350,000.")
![Dot Blot: Histone H3 [p Thr45] Antibody [NBP2-62795] - To test the cross reactivity of the antibody against H3T45p, an analysis was performed with peptides containing other histone modifications and the unmodified H3T45. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. This figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr45]-Antibody-Dot-Blot-NBP2-62795-img0002.jpg)
![Western Blot: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis using AHH3 mouse mAb against NIH3T3 (1), Hela (2), K562 (3) cell lysate.](http://images.novusbio.com/fullsize/HIST3H3-Antibody-4E9B11-Western-Blot-NBP2-52482-img0003.jpg "Western Blot: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis using AHH3 mouse mAb against NIH3T3 (1), Hela (2), K562 (3) cell lysate.")
![Flow Cytometry: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis of NIH/3T3 cells using HH3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HIST3H3-Antibody-4E9B11-Flow-Cytometry-NBP2-52482-img0001.jpg)
![ELISA: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/HIST3H3-Antibody-4E9B11-ELISA-NBP2-52482-img0002.jpg)
