Granzyme B Antibodies
We offer Granzyme B Antibodies for use in common research applications: ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Neutralization, Sandwich ELISA, Simple Western, Western Blot. Each Granzyme B Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Granzyme B Antibodies can be used in a variety of model species: Human, Monkey, Mouse, Primate, Rat. Use the list below to choose the Granzyme B Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Granzyme B polyclonal antibodies and browse our Granzyme B monoclonal antibody catalog.
Alternate Names for Granzyme B Antibodies
anti-Granzyme B antibody, anti-GZMB antibody
21 Results for "granzyme-b" in Primary Antibodies
![Western Blot: Granzyme B Antibody [NBP2-59678] - Total protein from human spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Granzyme B in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Western-Blot-NBP2-59678-img0002.jpg "Western Blot: Granzyme B Antibody [NBP2-59678] - Total protein from human spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Granzyme B in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP2-59678] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human tonsil (NBP2-30207) using Granzyme B antibody at 1:500 dilution. The primary antibody bound to antigens was detected using HRP conjugated secondary antibody which followed staining with DAB and counterstained with hematoxylin. The antibody generated a specific punctate staining pattern in the cytoplasm of a subset of cells (apparently cytotoxic lymphocytes) in the tested tonsil tissue.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP2-59678-img0001.jpg)
![Flow Cytometry: Granzyme B Antibody [NBP2-59678] - An intracellular stain was performed on U937 cells with NBP2-59678 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Flow-Cytometry-NBP2-59678-img0004.jpg)
![Flow Cytometry: Granzyme B Antibody [NBP2-59678] - An intracellular stain was performed on Raw264.7 cells with NBP2-59678 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Flow-Cytometry-NBP2-59678-img0003.jpg)
![Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - An intracellular stain was performed on U937 cells with Granzyme B [B18.1] Antibody NBP1-97525AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-B18-1-Flow-Cytometry-NBP1-97525-img0006.jpg "Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - An intracellular stain was performed on U937 cells with Granzyme B [B18.1] Antibody NBP1-97525AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: Granzyme B Antibody (B18.1) [NBP1-97525] - Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-B18-1-Western-Blot-NBP1-97525-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Granzyme B Antibody (B18.1) [NBP1-97525] - Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 ug/ml of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-B18-1-Immunocytochemistry-Immunofluorescence-NBP1-97525-img0005.jpg)
![Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - [FITC] Detection of granzyme B in permeabilized human CD8+ T lymphocytes (filled profile). Isotype control in combination with a secondary FITC-labeled antibody is shown as control. Method: Permeabilized human T cells (5 x 10^5).](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-B18-1-Flow-Cytometry-NBP1-97525-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Granzyme B Antibody [NB100-684] - Staining of Granzyme B in red on FFPE human Tonsil. Secondary antibody is donkey anti-rabbit A647. Nuclei counter-stained with DAPI. Staining pattern is cytoplasmic with obvious polarization (see arrowheads) in some cases. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Immunocytochemistry-NB100-684-img0002.jpg "Immunocytochemistry/Immunofluorescence: Granzyme B Antibody [NB100-684] - Staining of Granzyme B in red on FFPE human Tonsil. Secondary antibody is donkey anti-rabbit A647. Nuclei counter-stained with DAPI. Staining pattern is cytoplasmic with obvious polarization (see arrowheads) in some cases. This image was submitted via customer Review.")
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NB100-684] - FFPE human tonsil stained with Granzyme B antibody.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NB100-684-img0001.jpg)
![Flow Cytometry: Granzyme B Antibody (CLB-GB11) [NBP1-50071] - Separation of Granzyme B positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using anti-human Granzyme B (CLB-GB11) purified antibody (concentration in sample 3 ug/ml, GAM FITC).](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-CLB-GB11-Flow-Cytometry-NBP1-50071-img0003.jpg "Flow Cytometry: Granzyme B Antibody (CLB-GB11) [NBP1-50071] - Separation of Granzyme B positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using anti-human Granzyme B (CLB-GB11) purified antibody (concentration in sample 3 ug/ml, GAM FITC).")
![Flow Cytometry: Granzyme B Antibody (CLB-GB11) [NBP1-50071] - Analysis of human CD8+ peripheral blood cells with anti-human granzyme B (CLB-GB11) PE.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-CLB-GB11-Flow-Cytometry-NBP1-50071-img0001.jpg)
![Flow (Intracellular): Granzyme B Antibody (CLB-GB11) [NBP1-50071] - Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) purified antibody (concentration in sample 3 ug/ml, GAM FITC).](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-CLB-GB11-Flow-Intracellular-NBP1-50071-img0002.jpg)
![Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414] - Myc-tagged Granzyme H and B overexpressing cell lysate (15 ug) prepared using NETN lysis buffer. Antibody: Rabbit anti-Granzyme B recombinantmonoclonal antibody [BLR022E] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-myc antibody.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-BLR022E-Western-Blot-NBP2-76414-img0009.jpg "Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414] - Myc-tagged Granzyme H and B overexpressing cell lysate (15 ug) prepared using NETN lysis buffer. Antibody: Rabbit anti-Granzyme B recombinantmonoclonal antibody [BLR022E] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-myc antibody.")
![Immunocytochemistry/Immunofluorescence: Granzyme B Antibody (BLR022E) [NBP2-76414] - FFPE section of Granzyme B over-expressing cells (left) and Granzyme H overexpressing cells (right). Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-BLR022E-Immunocytochemistry-Immunofluorescence-NBP2-76414-img0010.jpg)
![Immunohistochemistry-Paraffin: Granzyme B Antibody (BLR022E) [NBP2-76414] - FFPE section of lung carcinoma. Antibody: Rabbit anti-Granzyme B recombinantmonoclonal antibody [BLR022E]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-BLR022E-Immunohistochemistry-Paraffin-NBP2-76414-img0012.jpg)
![Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414] - Whole cell lysate (15 ug) from HEK293T, HeLa, SR, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GranzymeB recombinantmonoclonal antibody [BLR022E] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-BLR022E-Western-Blot-NBP2-76414-img0008.jpg)
![Immunohistochemistry: Granzyme B Antibody (BLR022E) [NBP2-76414] - Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-BLR022E-Immunohistochemistry-NBP2-76414-img0007.jpg)
![Immunohistochemistry-Paraffin: Granzyme B Antibody (BLR022E) [NBP2-76414] - FFPE section of lymph node. Antibody: Rabbit anti-Granzyme B recombinantmonoclonal antibody [BLR022E]. Secondary: HRP-conjugated goat anti-rabbit IgG](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-BLR022E-Immunohistochemistry-Paraffin-NBP2-76414-img0011.jpg)
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining in human lymph node and skeletal muscle tissues using NBP1-88151 antibody. Corresponding GZMB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining in human lymph node and skeletal muscle tissues using NBP1-88151 antibody. Corresponding GZMB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Granzyme B Antibody [NBP1-88151] - Analysis in control (vector only transfected HEK293T lysate) and GZMB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Western-Blot-NBP1-88151-img0009.jpg)
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0010.jpg)
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0011.jpg)
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0012.jpg)
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0013.jpg)
![Western Blot: Granzyme B Antibody (OTI4E4) [NBP2-45732] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Granzyme B.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Western-Blot-NBP2-45732-img0004.jpg "Western Blot: Granzyme B Antibody (OTI4E4) [NBP2-45732] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Granzyme B.")
![Immunohistochemistry: Granzyme B Antibody (OTI4E4) [NBP2-45732] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Immunohistochemistry-NBP2-45732-img0003.jpg)
![Western Blot: Granzyme B Antibody (OTI4E4) [NBP2-45732] - Analysis of extracts (10ug) from 3 different cell lines.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Western-Blot-NBP2-45732-img0005.jpg)
![Immunohistochemistry: Granzyme B Antibody (OTI4E4) [NBP2-45732] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Immunohistochemistry-NBP2-45732-img0002.jpg)
![Immunofluorescence: Granzyme B Antibody (OTI4E4) [NBP2-45732] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Granzyme B.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Immunofluorescence-NBP2-45732-img0001.jpg)
![Western Blot: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Granzyme B.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Western-Blot-NBP2-70870-img0002.jpg "Western Blot: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Granzyme B.")
![Immunocytochemistry/Immunofluorescence: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Granzyme B.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70870-img0005.jpg)
![Immunohistochemistry: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70870-img0004.jpg)
![Western Blot: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870] - Analysis of extracts (10ug) from 3 different cell lines.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Western-Blot-NBP2-70870-img0001.jpg)
![Immunohistochemistry: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70870-img0003.jpg)
