Flotillin-1 Antibodies
We offer Flotillin-1 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each Flotillin-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Flotillin-1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Flotillin-1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Flotillin-1 polyclonal antibodies and browse our Flotillin-1 monoclonal antibody catalog.
Alternate Names for Flotillin-1 Antibodies
anti-Flotillin-1 antibody, anti-FLOT1 antibody, anti-flotillin 1 antibody, anti-integral membrane component of caveolae antibody
9 Results for "flotillin-1" in Primary Antibodies
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Analysis in human adrenal gland and skin tissues using NBP1-87498 antibody. Corresponding FLOT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-2472024125914..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Analysis in human adrenal gland and skin tissues using NBP1-87498 antibody. Corresponding FLOT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-FLOT1 antibody. Corresponding FLOT1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0012.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0009.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-NBP1-87498-img0013.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413032..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413122..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413216..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Skin shows very weak membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-24720241338..jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-79022-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Simple-Western-NBP1-79022-img0003.jpg)
![Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252844.jpg "Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunoprecipitation: Flotillin-1 Antibody [NBP3-38231] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug Flotillin-1 antibody. Western blot was performed from the immunoprecipitate using Flotillin-1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-1012202411545540.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121097.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Rat heart using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252843.jpg)
![Immunocytochemistry/ Immunofluorescence: Flotillin-1 Antibody [NBP3-38231] - Immunofluorescence analysis of NIH/3T3 cells using Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121062.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western Blot analysis of lysates from wild type (WT) and Flotillin-1 knockout (KO) 293T cells, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-311202516593618.jpg)
![Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Analysis of extracts of various cell lines, using Flotillin-1 Rabbit mAb (NBP3-16152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-8I2S7-Western-Blot-NBP3-16152-img0004.jpg "Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Analysis of extracts of various cell lines, using Flotillin-1 Rabbit mAb (NBP3-16152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Analysis of extracts of various cell lines, using Flotillin-1 Rabbit mAb (NBP3-16152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-8I2S7-Western-Blot-NBP3-16152-img0005.jpg)
![Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16152_rabbit-flotillin-1-mab-8i2s7-5220251215429.jpg)
![Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16152_rabbit-flotillin-1-mab-8i2s7-52202511365156.jpg)
![Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16152_rabbit-flotillin-1-mab-8i2s7-52202511365175.jpg)
![Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [Flotillin-1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16152_rabbit-flotillin-1-mab-8i2s7-5220251723573.jpg)
![Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [Flotillin-1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16152_rabbit-flotillin-1-mab-8i2s7-52202517273526.jpg)
![Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [Flotillin-1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16152_rabbit-flotillin-1-mab-8i2s7-52202517192981.jpg)
![Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NB100-1043-img0001.jpg "Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.")
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0003.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0002.jpg)
![Western Blot: Flotillin-1 Antibody [NBP3-37979] - Western blot analysis of various lysates using Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-37979_rabbit-flotillin-1-pab-1012202411353061.jpg "Western Blot: Flotillin-1 Antibody [NBP3-37979] - Western blot analysis of various lysates using Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-37979] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37979_rabbit-flotillin-1-pab-1012202411343364.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-37979] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37979_rabbit-flotillin-1-pab-1012202411392922.jpg)
![Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508] - IP samples: HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunoprecipitation-NBP2-16508-img0003.jpg "Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508] - IP samples: HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Western Blot: Flotillin-1 Antibody [NBP2-16508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp2-16508_rabbit-polyclonal-flotillin-1-antibody-western-blot-8620239419..jpg)
![Western Blot: Flotillin-1 Antibody [H00010211-D01P] - Analysis of FLOT1 expression in human liver.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-H00010211-D01P-img0003.jpg "Western Blot: Flotillin-1 Antibody [H00010211-D01P] - Analysis of FLOT1 expression in human liver.")
![Western Blot: Flotillin-1 Antibody [H00010211-D01P] - Analysis of FLOT1 expression in transfected 293T cell line by FLOT1 polyclonal antibody.Lane 1: FLOT1 transfected lysate(47.40 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-H00010211-D01P-img0004.jpg)
![Western Blot: Flotillin-1 Antibody (S08-0G5) [NBP3-14932] - Western blot detection of Flotillin-1 in Hela, A549, MCF-7, U251 cell lysates using Flotillin-1 Rabbit mAb (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 49kDa.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-S08-0G5-Western-Blot-NBP3-14932-img0001.jpg "Western Blot: Flotillin-1 Antibody (S08-0G5) [NBP3-14932] - Western blot detection of Flotillin-1 in Hela, A549, MCF-7, U251 cell lysates using Flotillin-1 Rabbit mAb (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 49kDa.")