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DNER Antibodies

We offer DNER Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each DNER Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DNER Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the DNER Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DNER polyclonal antibodies and browse our DNER monoclonal antibody catalog.

Alternate Names for DNER Antibodies

anti-DNER antibody, anti-bet antibody, anti-delta and Notch-like epidermal growth factor-related receptor antibody, anti-delta-notch-like EGF repeat-containing transmembrane antibody, anti-delta/notch-like EGF repeat containing antibody, anti-H_NH0150O02.1 antibody, anti-UNQ26 antibody, anti-WUGSC:H_NH0150O02.1 antibody

9 Results for "dner" in Primary Antibodies

AF2254

DNER Antibody [Unconjugated]

(12 Publications)

100 μg

AF2254

DNER was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2254) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons and molecular layer. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

DNER does not activate, function with, or bind to Notch, but known Notch ligand DLL1 does.(A) Pooled luciferase results from 4 separate experiments (normalized to the mean of empty vector in each experiment). U2OS cells were transfected with ligand (DLL1, DNER, or EV), and separately a population of U2OS cells was transfected to express Notch, the control luciferase Renilla, and TP1, a promoter that expresses firefly luciferase when Notch is activated. The two populations were co-cultured 24 hours after transfection (trans configuration), and activity read after an additional 24–48 hours of incubation. (B) C2C12 cells (myoblasts) were incubated with differentiation media (2% horse serum) that either had pre-clustered DLL1-fc (1:1), pre-clustered DNER-fc (1:1), un-clustered DNER-fc, or fc only, all at a ratio of 1:150 in media. Cells were incubated for 72 hours, then fixed, and stained for the presence of myosin heavy chain (MHC) and nuclei. By measuring the percent of total nuclei that were inside of differentiated MHC positive myotubes, fusion indexes were calculated. (C) DNER (top left, green) transfected U2OS cells were not labeled by pre-clustered Notch-fc (top middle, red) but DLL1 (bottom left, green) transfected U2OS cells were labeled by pre-clustered Notch-fc (bottom middle, red). Merged images are shown at far right. Scale 10 μM. **** = p value <0.0001. *** = p value 0.002. ns = not significant. DLL1 = Delta-like 1, a known Notch Ligand, DNER = Delta/Notch-like epidermal growth factor (EGF) related receptor, GSI = gamma -secretase inhibitor, fc only = rabbit anti-human-fc. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0161157), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2254

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2254-SP

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AF3646

DNER Antibody

(1 Review)

(1 Publication)

100 μg

AF3646

Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3646) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF3646

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3646-SP

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MAB2254

DNER Antibody (298306)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAM2254

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2254-SP

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NBP1-79415

DNER Antibody

100ul

NBP1-79415

Western Blot: DNER Antibody [NBP1-79415] - HepG2 cell lysate, concentration 5.0ug/ml.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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H00092737-M02

DNER Antibody (2F3)

0.1 mg

H00092737-M02

Western Blot: DNER Antibody (2F3) [H00092737-M02] - detection against Immunogen (37.73 KDa) .

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00092737-M03

DNER Antibody (4E8)

0.1 mg

H00092737-M03

Western Blot: DNER Antibody (4E8) [H00092737-M03] - DNER monoclonal antibody (M03), clone 4E8 Western Blot analysis of DNER expression in PC-12 ( Cat # L012V1 ).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-88665

DNER Antibody

(1 Review)

0.1 ml

NBP1-88665

Immunohistochemistry-Paraffin: DNER Antibody [NBP1-88665] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in purkinje cells.

Immunohistochemistry-Paraffin: DNER Antibody [NBP1-88665] - Staining of human placenta shows no positivity in trophoblastic cells as expected.

Immunohistochemistry-Paraffin: DNER Antibody [NBP1-88665] - Staining of human small intestine shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: DNER Antibody [NBP1-88665] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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BAF3646

DNER Antibody [Biotin]

(1 Publication)

50 μg

BAF3646

Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3646) followed by streptavidin-HRP. A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Biotin

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3646
Carrier Free	AF3646-SP

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BAF2254

DNER Antibody [Biotin]

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Biotin

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2254
Carrier Free	AF2254-SP

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