DLG1 Antibodies
We offer DLG1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each DLG1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DLG1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the DLG1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DLG1 polyclonal antibodies and browse our DLG1 monoclonal antibody catalog.
Alternate Names for DLG1 Antibodies
anti-DLG1 antibody, anti-discs, large homolog 1 (Drosophila) antibody, anti-disks large homolog 1 antibody, anti-DKFZp761P0818 antibody, anti-DKFZp781B0426 antibody, anti-hDlg antibody, anti-large (Drosophila) homolog 1 antibody, anti-presynaptic protein SAP97 antibody
9 Results for "dlg1" in Primary Antibodies
![Western Blot: DLG1 Antibody [NB600-1229] - Analysis of 25 ug of C6 (lane 1), PC12 (lane 2) and rat brain (lane 3) cell lysates.](http://images.novusbio.com/fullsize/DLG1-Antibody-Western-Blot-NB600-1229-img0003.jpg "Western Blot: DLG1 Antibody [NB600-1229] - Analysis of 25 ug of C6 (lane 1), PC12 (lane 2) and rat brain (lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: DLG1 Antibody [NB600-1229] - Analysis of SAP97 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SAP97 polyclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/DLG1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1229-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DLG1 Antibody [NB600-1229] - Analysis of SAP97 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SAP97 polyclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/DLG1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1229-img0001.jpg)
![Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929] - Western blot analysis of DLG1 on different lysates with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-288202412442920.jpg "Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929] - Western blot analysis of DLG1 on different lysates with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DLG1 Antibody (JE39-74) [NBP3-32929] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-298202414161898.jpg)
![Flow Cytometry: DLG1 Antibody (JE39-74) [NBP3-32929] - Flow cytometric analysis of SiHa cells labeling DLG1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32929, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32929_rabbit-dlg1-mab-je39-74-392024884663.jpg)
![Immunocytochemistry/ Immunofluorescence: DLG1 Antibody [NBP3-38560] - Immunofluorescence analysis of L929 cells using DLG1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38560_rabbit-dlg1-pab-1012202411535825.jpg "Immunocytochemistry/ Immunofluorescence: DLG1 Antibody [NBP3-38560] - Immunofluorescence analysis of L929 cells using DLG1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: DLG1 Antibody [NBP3-38560] - Western blot analysis of various lysates using DLG1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38560_rabbit-dlg1-pab-101220241243910.jpg)
![Western Blot: DLG1 Antibody [NBP1-20907] - (0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DLG1-Antibody-Western-Blot-NBP1-20907-img0002.jpg "Western Blot: DLG1 Antibody [NBP1-20907] - (0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: DLG1 Antibody (S06-6C8) [NBP3-20002] - Western blot detection of DLG1 in Hela, A549, HL-60 using NBP3-20002 (1:1000 diluted)](http://images.novusbio.com/fullsize/DLG1-Antibody-S06-6C8-Western-Blot-NBP3-20002-img0001.jpg "Western Blot: DLG1 Antibody (S06-6C8) [NBP3-20002] - Western blot detection of DLG1 in Hela, A549, HL-60 using NBP3-20002 (1:1000 diluted)")
![Western Blot: DLG1 Antibody [NBP1-26611] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-SAP97 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SAP97 was also immunoprecipitated by another rabbit anti-SAP97 antibody, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/DLG1-Antibody-Western-Blot-NBP1-26611-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DLG1 Antibody [NBP1-26611] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-SAP97 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SAP97 was also immunoprecipitated by another rabbit anti-SAP97 antibody, which recognizes a downstream epitope.")
![Western Blot: DLG1 Antibody [NBP2-22474] - Analysis of SAP97 on extract from COS cells transfected with rat SAP97.](http://images.novusbio.com/fullsize/DLG1-Antibody-Western-Blot-NBP2-22474-img0002.jpg "Western Blot: DLG1 Antibody [NBP2-22474] - Analysis of SAP97 on extract from COS cells transfected with rat SAP97.")
![Western Blot: DLG1 Antibody (1B12) [H00001739-M03] - Detection against Immunogen (35.64 KDa) .](http://images.novusbio.com/fullsize/DLG1-Antibody-1B12-Western-Blot-H00001739-M03-img0001.jpg "Western Blot: DLG1 Antibody (1B12) [H00001739-M03] - Detection against Immunogen (35.64 KDa) .")