Cytochrome P450 17A1 Antibodies
We offer Cytochrome P450 17A1 Antibodies for use in common research applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each Cytochrome P450 17A1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Cytochrome P450 17A1 Antibodies can be used in a variety of model species: Human. Use the list below to choose the Cytochrome P450 17A1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Cytochrome P450 17A1 polyclonal antibodies and browse our Cytochrome P450 17A1 monoclonal antibody catalog.
Alternate Names for Cytochrome P450 17A1 Antibodies
anti-Cytochrome P450 17A1 antibody, anti-CYP17A1 antibody, anti-CPT7 antibody, anti-cyp17 antibody, anti-cytochrome p450 XVIIA1 antibody, anti-cytochrome P450, family 17, subfamily A, polypeptide 1 antibody, anti-cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenalhyperplasia antibody, anti-Cytochrome P450-C17 antibody, anti-Cytochrome P450c17 antibody, anti-EC 1.14.99 antibody, anti-P450C17 antibody, anti-P450C17CYPXVII antibody, anti-S17AH antibody
12 Results for "cytochrome-p450-17a1" in Primary Antibodies
![Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 17A1.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Western-Blot-NBP2-01151-img0024.jpg "Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 17A1.")
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-OTI3F11-Immunohistochemistry-NBP2-01151-img0026.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Jurkat cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Flow-Cytometry-NBP2-01151-img0014.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0015.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0016.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0017.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0018.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human breast tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0019.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0020.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0021.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0022.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human tonsil using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Immunohistochemistry-Paraffin-NBP2-01151-img0023.jpg)
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in 13 year old testis. Immunoexpression in Leydig cell. Image from a verified customer review.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-OTI3F11-Immunohistochemistry-NBP2-01151-img0025.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Hela cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F11-Flow-Cytometry-NBP2-01151-img0013.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of Cytochrome P450 17A1 on rat testis tissue lysates with Rabbit anti-Cytochrome P450 17A1 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Western-Blot-NBP2-75461-img0008.jpg "Western Blot: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of Cytochrome P450 17A1 on rat testis tissue lysates with Rabbit anti-Cytochrome P450 17A1 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0007.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of SH-SY-5Y cells with Cytochrome P450 17A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Flow-Cytometry-NBP2-75461-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded human breast tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0006.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody [NBP2-13892] - Analysis in human adrenal gland tissue.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NBP2-13892-img0003.jpg "Western Blot: Cytochrome P450 17A1 Antibody [NBP2-13892] - Analysis in human adrenal gland tissue.")
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining of human pancreas shows no positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP17A1 antibody. Corresponding CYP17A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining in human testis and pancreas tissues.. Corresponding CYP17A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NBP2-13892] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NBP2-13892-img0011.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody [NB100-2842] - (0.3ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NB100-2842-img0004.jpg "Western Blot: Cytochrome P450 17A1 Antibody [NB100-2842] - (0.3ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody [NB100-2842] - (3.8ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Immunohistochemistry-Paraffin-NB100-2842-img0003.jpg)
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-2722024831378.jpg "Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Positive Western Blot detected in: HepG2 whole cell lysate, Rat Heart whole cell lysate, Rat Brain whole cell lysate. All lanes: Cytochrome P450 17A1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 kDa Observed band size: 58 kDa](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-28220241140920.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Staining of Hela Cells with Cytochrome P450 17A1 Antibody (5A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26637_rabbit-cytochrome-p450-17a1-mab-5a9-282202412475149.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NBP2-16077-img0003.jpg "Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.")
![Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NBP2-16077-img0001.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-Western-Blot-NBP2-16077-img0002.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-5D2H1-Western-Blot-NBP3-16541-img0002.jpg "Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Immunofluorescence analysis of HeLa cells using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-5D2H1-Immunocytochemistry-Immunofluorescence-NBP3-16541-img0001.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody [NBP3-35383] - Western blot analysis of lysates from rat testis, using Cytochrome P450 17A1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35383_rabbit-cytochrome-p450-17a1-pab-1012202411592881.jpg "Western Blot: Cytochrome P450 17A1 Antibody [NBP3-35383] - Western blot analysis of lysates from rat testis, using Cytochrome P450 17A1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: Cytochrome P450 17A1 Antibody (3F12) [NBP2-46418] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 .](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-3F12-Western-Blot-NBP2-46418-img0001.jpg "Western Blot: Cytochrome P450 17A1 Antibody (3F12) [NBP2-46418] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 .")
![Western Blot: Cytochrome P450 17A1 Antibody [NBP3-35404] - Western blot analysis of lysates from rat testis, using Cytochrome P450 17A1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35404_rabbit-cytochrome-p450-17a1-pab-10122024120623.jpg "Western Blot: Cytochrome P450 17A1 Antibody [NBP3-35404] - Western blot analysis of lysates from rat testis, using Cytochrome P450 17A1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")