COMT Antibodies
We offer COMT Antibodies for use in common research applications: ELISA, Enzyme Immunoassay, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each COMT Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our COMT Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the COMT Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our COMT polyclonal antibodies and browse our COMT monoclonal antibody catalog.
Alternate Names for COMT Antibodies
anti-COMT antibody, anti-catechol O-methyltransferase antibody, anti-catechol-O-methyltransferase antibody, anti-EC 2.1.1.6 antibody
9 Results for "comt" in Primary Antibodies
![Western Blot: COMT Antibody [NB100-1780] - Staining of MCF7 (A) with antibody at 0.5 ug/mL, U251 cell lysate 1 (B) and U251 cell lysate 2 (C) (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NB100-1780-img0002.jpg "Western Blot: COMT Antibody [NB100-1780] - Staining of MCF7 (A) with antibody at 0.5 ug/mL, U251 cell lysate 1 (B) and U251 cell lysate 2 (C) (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: COMT Antibody [NB100-1780] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/COMT-Antibody-Flow-Cytometry-NB100-1780-img0003.jpg)
![Western Blot: COMT Antibody [NB100-1780] - Staining of Human Liver (A) with antibody at 2 ug/mL and Ovary (B) lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NB100-1780-img0001.jpg)
![Western Blot: COMT Antibody [NBP2-15954] - Wild-type (WT) and COMT knockout (KO) HEK293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with COMT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NBP2-15954-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: COMT Antibody [NBP2-15954] - Wild-type (WT) and COMT knockout (KO) HEK293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with COMT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954] - Paraffin-embedded rat brain. COMT antibody diluted at 1:500.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NBP2-15954-img0004.jpg)
![Western Blot: COMT Antibody [NBP2-15954] - COMT antibody detects COMT protein by Western blot analysis. A. 30 ug K562 whole cell lysate/extract 12 % SDS-PAGECOMT antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NBP2-15954-img0001.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954] - Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NBP2-15954-img0002.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954] - Paraffin-embedded Rat brain. COMT antibody diluted at 1:500.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NBP2-15954-img0003.jpg)
![Western Blot: COMT Antibody [NB100-41387] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NB100-41387-img0007.jpg "Western Blot: COMT Antibody [NB100-41387] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0006.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Cerebellum. Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0004.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0005.jpg)
![Western Blot: COMT Antibody [NBP1-85818] - Analysis in human cell lines MCF-7 and PC-3 using Anti-COMT antibody. Corresponding COMT RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NBP1-85818-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: COMT Antibody [NBP1-85818] - Analysis in human cell lines MCF-7 and PC-3 using Anti-COMT antibody. Corresponding COMT RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: COMT Antibody [NBP1-85818] - Staining in human placenta and pancreas tissues using anti-COMT antibody. Corresponding COMT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NBP1-85818-img0014.jpg)
![Western Blot: COMT Antibody [NBP1-85818] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NBP1-85818-img0008.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NBP1-85818] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NBP1-85818-img0010.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NBP1-85818] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NBP1-85818-img0013.jpg)
![Western Blot: COMT Antibody (1G4-1A1) [H00001312-M01] - Analysis of COMT expression in transfected 293T cell line by COMT monoclonal antibody (M01), clone 1G4-1A1.Lane 1: COMT transfected lysate(24.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/COMT-Antibody-1G4-1A1-Western-Blot-H00001312-M01-img0005.jpg "Western Blot: COMT Antibody (1G4-1A1) [H00001312-M01] - Analysis of COMT expression in transfected 293T cell line by COMT monoclonal antibody (M01), clone 1G4-1A1.Lane 1: COMT transfected lysate(24.4 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: COMT Antibody (1G4-1A1) [H00001312-M01] - COMT monoclonal antibody (M01), clone 1G4-1A1 Analysis of COMT expression in A-431.](http://images.novusbio.com/fullsize/COMT-Antibody-1G4-1A1-Western-Blot-H00001312-M01-img0004.jpg)
![Sandwich ELISA: COMT Antibody (1G4-1A1) [H00001312-M01] - Detection limit for recombinant GST tagged COMT is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/COMT-Antibody-1G4-1A1-Sandwich-ELISA-H00001312-M01-img0003.jpg)
![Immunohistochemistry: COMT Antibody (8E1) [NBP3-26646] - Image of COMT Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26646_rabbit-comt-mab-8e1-262202415355741.jpg "Immunohistochemistry: COMT Antibody (8E1) [NBP3-26646] - Image of COMT Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: COMT Antibody (8E1) [NBP3-26646] - Positive Western Blot detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate. All lanes: COMT Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 31, 25 kDa Observed band size: 31 kDa](http://images.novusbio.com/fullsize/nbp3-26646_rabbit-comt-mab-8e1-282202411363016.jpg)
![Western Blot: COMT Antibody (9Y2A3) [NBP3-16413] - Western blot analysis of extracts of various cell lines, using COMT Rabbit mAb (NBP3-16413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/COMT-Antibody-9Y2A3-Western-Blot-NBP3-16413-img0001.jpg "Western Blot: COMT Antibody (9Y2A3) [NBP3-16413] - Western blot analysis of extracts of various cell lines, using COMT Rabbit mAb (NBP3-16413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: COMT Antibody (9Y2A3) [NBP3-16413] - Western blot analysis of extracts of various cell lines, using COMT Rabbit mAb (NBP3-16413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/COMT-Antibody-9Y2A3-Western-Blot-NBP3-16413-img0002.jpg)
![Western Blot: COMT Antibody [H00001312-B01P] - Analysis of COMT expression in human placenta.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-H00001312-B01P-img0004.jpg "Western Blot: COMT Antibody [H00001312-B01P] - Analysis of COMT expression in human placenta.")
![Western Blot: COMT Antibody [H00001312-B01P] - Analysis of COMT expression in transfected 293T cell line by COMT polyclonal antibody. Lane 1: COMT transfected lysate(24.31 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-H00001312-B01P-img0005.jpg)