CENPF Antibodies
We offer CENPF Antibodies for use in common research applications: Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each CENPF Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CENPF Antibodies can be used in a variety of model species: Bovine, Human. Use the list below to choose the CENPF Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CENPF polyclonal antibodies.
Alternate Names for CENPF Antibodies
anti-CENPF antibody, anti-AH antigen antibody, anti-cell-cycle-dependent 350K nuclear protein antibody, anti-CENF antibody, anti-CENP-F antibody, anti-CENP-F kinetochore protein antibody, anti-centromere protein F (350/400kD, mitosin) antibody, anti-centromere protein F antibody, anti-centromere protein F, 350/400ka (mitosin) antibody, anti-centromere protein F, 350/400kDa (mitosin) antibody, anti-hcp-1 antibody, anti-Kinetochore protein CENPF antibody, anti-mitosin antibody, anti-PRO1779 antibody
7 Results for "cenpf" in Primary Antibodies
![Knockdown Validated: CENPF Antibody - BSA Free [NB500-101] - Kinetochore localization of RZZ and MAD1 are independent of CENP-E and CENP-F. CENP-F depletion does not interfere with the recruitment of Zwilch. The graphs show mean intensity of one (B, C, and E), two (D and F), or three (A, G, and H) experiments; the error bars indicate S.E., and the mean values for nondepleted cells are set to 1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29748388/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunohistochemistry-Paraffin-NB500-101-img0009.jpg "<b>Biological Strategies Validation. </b>Knockdown Validated: CENPF Antibody - BSA Free [NB500-101] - Kinetochore localization of RZZ and MAD1 are independent of CENP-E and CENP-F. CENP-F depletion does not interfere with the recruitment of Zwilch. The graphs show mean intensity of one (B, C, and E), two (D and F), or three (A, G, and H) experiments; the error bars indicate S.E., and the mean values for nondepleted cells are set to 1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29748388/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.](http://images.novusbio.com/fullsize/CENPF-Antibody-Western-Blot-NB500-101-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CENPF Antibody [NB500-101] - Distribution of CENP-F (red), largely associated with the mitotic spindle and a clearly distinguishable frac.](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunocytochemistry-Immunofluorescence-NB500-101-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CENPF Antibody [NB500-101] - CENPF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunocytochemistry-Immunofluorescence-NB500-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CENPF Antibody [NB500-101] - Confocal immunofluorescent analysis of HeLa cells using CENPF antibody (NB500-101, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunocytochemistry-Immunofluorescence-NB500-101-img0007.jpg)
![Western Blot: CENPF Antibody (PSH0-79) [NBP3-32181] - Western blot analysis of CENPF on different lysates with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 358 kDa Observed band size: 358 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32181) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32181_rabbit-cenpf-mab-psh0-79-278202416331287.jpg "Western Blot: CENPF Antibody (PSH0-79) [NBP3-32181] - Western blot analysis of CENPF on different lysates with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 358 kDa Observed band size: 358 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32181) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CENPF Antibody (PSH0-79) [NBP3-32181] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32181) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32181_rabbit-cenpf-mab-psh0-79-298202414262657.jpg)
![Immunohistochemistry-Paraffin: CENPF Antibody [NBP2-62641] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunohistochemistry-Paraffin-NBP2-62641-img0002.jpg "Immunohistochemistry-Paraffin: CENPF Antibody [NBP2-62641] - Staining of human testis shows high expression.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CENPF Antibody [NBP2-62641] - Immunohistochemistry analysis in human testis and liver tissues using Anti-CENPF antibody. Corresponding CENPF RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunohistochemistry-Paraffin-NBP2-62641-img0001.jpg)
![Immunohistochemistry-Paraffin: CENPF Antibody [NBP2-62641] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunohistochemistry-Paraffin-NBP2-62641-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CENPF Antibody (7F11.2) [NBP3-13730] - Immunofluorescent staining of mitotic U2OS cells with mitosin/CENP-F antibody NBP3-13730 (green) at 1:500 dilution. Cells were pre-extracted with ice-cold CSK buffer (0.5% Triton X-100, 300 mM Sucrose, 10 mM PIPES, pH8.0) for 4 min, followed by fixation with 4% PFA and regular IFA procedure. Blue channel (DAPI staining) shows chromosomes.](http://images.novusbio.com/fullsize/CENPF-Antibody-7F11-2-Immunocytochemistry-Immunofluorescence-NBP3-13730-img0001.jpg "Immunocytochemistry/Immunofluorescence: CENPF Antibody (7F11.2) [NBP3-13730] - Immunofluorescent staining of mitotic U2OS cells with mitosin/CENP-F antibody NBP3-13730 (green) at 1:500 dilution. Cells were pre-extracted with ice-cold CSK buffer (0.5% Triton X-100, 300 mM Sucrose, 10 mM PIPES, pH8.0) for 4 min, followed by fixation with 4% PFA and regular IFA procedure. Blue channel (DAPI staining) shows chromosomes.")
![Immunohistochemistry: CENPF Antibody (7F11.2) [NBP3-13730] - High magnification photomicrograph of an NG3 tumor with a high percentage of mitosin-positive nuclei, indicative of high proliferative activity.](http://images.novusbio.com/fullsize/CENPF-Antibody-7F11-2-Immunohistochemistry-NBP3-13730-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CENPF Antibody [NBP2-56124] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CENPF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56124-img0001.jpg "Immunocytochemistry/Immunofluorescence: CENPF Antibody [NBP2-56124] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
