CD58/LFA-3 Antibodies
We offer CD58/LFA-3 Antibodies for use in common research applications: Blocking/Neutralizing, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Sandwich ELISA, Western Blot. Each CD58/LFA-3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CD58/LFA-3 Antibodies can be used in a variety of model species: Human, Sheep. Use the list below to choose the CD58/LFA-3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CD58/LFA-3 polyclonal antibodies and browse our CD58/LFA-3 monoclonal antibody catalog.
Alternate Names for CD58/LFA-3 Antibodies
anti-CD58/LFA-3 antibody, anti-CD58 antibody, anti-Ag3 antibody, anti-CD58 antigen antibody, anti-CD58 antigen, (lymphocyte function-associated antigen 3) antibody, anti-CD58 molecule antibody, anti-FLJ23181 antibody, anti-FLJ43722 antibody, anti-LFA-3 antibody, anti-LFA3ag3 antibody, anti-lymphocyte function-associated antigen 3 antibody, anti-Surface glycoprotein LFA-3 antibody
18 Results for "cd58-lfa-3" in Primary Antibodies
![Western Blot: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-TS2-9-Western-Blot-NBP2-22542-img0005.jpg "Western Blot: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) and a molecular weight protein ladder.")
![Immunocytochemistry/Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 (green) showing staining in the cytoplasm of Raji cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-TS2-9-Immunocytochemistry-Immunofluorescence-NBP2-22542-img0004.jpg)
![Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in BAF-3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-TS2-9-Flow-Cytometry-NBP2-22542-img0003.jpg)
![Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in PBMC cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-TS2-9-Flow-Cytometry-NBP2-22542-img0001.jpg)
![Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in PBMC cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-TS2-9-Flow-Cytometry-NBP2-22542-img0002.jpg)
![Western Blot: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - CD58 monoclonal antibody (M01), clone 2D11-B10 Analysis of CD58 expression in Jurkat.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-2D11-B10-Western-Blot-H00000965-M01-img0013.jpg "Western Blot: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - CD58 monoclonal antibody (M01), clone 2D11-B10 Analysis of CD58 expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - Analysis of monoclonal antibody to CD58 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-2D11-B10-Immunocytochemistry-Immunofluorescence-H00000965-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - Analysis of monoclonal antibody to CD58 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-2D11-B10-Immunohistochemistry-Paraffin-H00000965-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - Analysis of monoclonal antibody to CD58 on formalin-fixed paraffin-embedded human lymphoma tissue Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-2D11-B10-Immunohistochemistry-Paraffin-H00000965-M01-img0010.jpg)
![ELISA: CD58/LFA-3 Antibody (2D11-B10) [H00000965-M01] - Detection limit for recombinant GST tagged CD58 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-2D11-B10-ELISA-H00000965-M01-img0006.jpg)
![Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-085-Western-Blot-NBP2-90096-img0001.jpg "Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-CD58 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD58 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 61 kDa](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-085-Immunoprecipitation-NBP2-90096-img0002.jpg)
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human tonsil with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-126-Immunohistochemistry-Paraffin-NBP2-90097-img0002.jpg "Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human tonsil with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human appendix with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-126-Immunohistochemistry-Paraffin-NBP2-90097-img0001.jpg)
![Western Blot: CD58/LFA-3 Antibody (PSH02-74) [NBP3-32161] - Western blot analysis of CD58/LFA-3 on different lysates with Rabbit anti-CD58/LFA-3 antibody (NBP3-32161) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: Jurkat cell lysate Lane 4: K-562 cell lysate Lane 5: HUVEC cell lysate Lane 6: HT-29 cell lysate Lane 7: TF-1 cell lysate Lane 8: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 28 kDa Observed band size: 55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32161) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32161_rabbit-cd58-lfa-3-mab-psh02-74-278202416331221.jpg "Western Blot: CD58/LFA-3 Antibody (PSH02-74) [NBP3-32161] - Western blot analysis of CD58/LFA-3 on different lysates with Rabbit anti-CD58/LFA-3 antibody (NBP3-32161) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: Jurkat cell lysate Lane 4: K-562 cell lysate Lane 5: HUVEC cell lysate Lane 6: HT-29 cell lysate Lane 7: TF-1 cell lysate Lane 8: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 28 kDa Observed band size: 55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32161) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: CD58/LFA-3 Antibody (PSH02-74) [NBP3-32161] - Flow cytometric analysis of human peripheral blood lymphocytes labeling CD58/LFA-3. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32161, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (black). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-32161_rabbit-cd58-lfa-3-mab-psh02-74-29202416453261.jpg)
![Flow Cytometry: CD58/LFA-3 Antibody (MEM-63) [NB120-1420] - Separation of human monocytes (red-filled) from human CD58/LFA-3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD58/LFA-3 (MEM-63) purified antibody (concentration in sample 1.67 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-MEM-63-Flow-Cytometry-NB120-1420-img0002.jpg "Flow Cytometry: CD58/LFA-3 Antibody (MEM-63) [NB120-1420] - Separation of human monocytes (red-filled) from human CD58/LFA-3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD58/LFA-3 (MEM-63) purified antibody (concentration in sample 1.67 ug/ml, GAM APC).")
![Flow Cytometry: CD58/LFA-3 Antibody (MEM-63) [NB120-1420] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD58/LFA-3 (MEM-63) purified antibody (concentration in sample 1.67 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-MEM-63-Flow-Cytometry-NB120-1420-img0001.jpg)
![Western Blot: CD58/LFA-3 Antibody [NBP1-98401] - COLO205 Cell Lysate 1.0ug/ml, Gel Concentration: 12%](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-Western-Blot-NBP1-98401-img0002.jpg "Western Blot: CD58/LFA-3 Antibody [NBP1-98401] - COLO205 Cell Lysate 1.0ug/ml, Gel Concentration: 12%")
![Western Blot: CD58/LFA-3 Antibody (186) [NBP2-90099] - Lane A: Raji Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: TF-1 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per laneSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:28 kDa](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-186-Western-Blot-NBP2-90099-img0001.jpg "Western Blot: CD58/LFA-3 Antibody (186) [NBP2-90099] - Lane A: Raji Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: TF-1 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per laneSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:28 kDa")
![Western Blot: CD58/LFA-3 Antibody (185) [NBP2-90098] - Lane A: Raji Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: TF-1 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-185-Western-Blot-NBP2-90098-img0001.jpg "Western Blot: CD58/LFA-3 Antibody (185) [NBP2-90098] - Lane A: Raji Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: TF-1 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.")
![Western Blot: CD58/LFA-3 Antibody (13G5) [NBP3-26677] - Positive Western Blot detected in: Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate. All lanes: CD58/LFA-3 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 27, 26 kDa Observed band size: 55-70 kDa](http://images.novusbio.com/fullsize/nbp3-26677_rabbit-cd58-lfa-3-mab-13g5-282202411351624.jpg "Western Blot: CD58/LFA-3 Antibody (13G5) [NBP3-26677] - Positive Western Blot detected in: Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate. All lanes: CD58/LFA-3 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 27, 26 kDa Observed band size: 55-70 kDa")
![Flow Cytometry: CD58/LFA-3 Antibody (RTH32A) [NBP2-60929] - Obtained with rabbit leukocytes](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-RTH32A-Flow-Cytometry-NBP2-60929-img0001.jpg "Flow Cytometry: CD58/LFA-3 Antibody (RTH32A) [NBP2-60929] - Obtained with rabbit leukocytes")