CD19 Antibodies
CD19 (Cluster of Differentiation 19) is a type I transmembrane glycoprotein belonging to immunoglobulin (Ig) subfamily that is a biomarker for B cells and plays a role in B cell signaling and immune response. The human CD19 protein is 556 amino acids (aa) in length with a theoretical molecular weight of 61 kDa. We offer CD19 antibodies in our catalog have been cited in over 25+ publications, including 5-star customer reviewed antibodies. Browse our inventory of recombinant, monoclonal, polyclonal, and CD19-conjugated antibodies. Our CD19 antibodies have been validated for multiple research applications including Blocking/Neutralizing, CyTOF-ready, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, and Western Blot. These CD19 antibodies are reactive in Human, Rat, Mouse, Monkey, and Chimpanzee. Choose the anti-CD19 antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products. Each CD19 antibody is fully covered by Novus’ Guarantee+ to give you peace of mind and support when you need it.
Alternate Names for CD19 Antibodies
anti-CD19 antibody, anti-B-lymphocyte antigen CD19 antibody, anti-B-lymphocyte surface antigen B4 antibody, anti-B4 antibody, anti-CD19 antigen antibody, anti-CD19 molecule antibody, anti-CVID3 antibody, anti-Differentiation antigen CD19 antibody, anti-MGC12802 antibody, anti-T-cell surface antigen Leu-12 antibody
61 Results for "cd19" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - CD19 antibody (CB19) was tested in Non-Hodgkin's lymphoma cells at 1:200 dilution. Green: Alexa Flour 488. Image from verified customer review.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Immunocytochemistry-Immunofluorescence-NBP2-25196-img0014.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - CD19 antibody (CB19) was tested in Non-Hodgkin's lymphoma cells at 1:200 dilution. Green: Alexa Flour 488. Image from verified customer review.")
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Immunocytochemistry-Immunofluorescence-NBP2-25196-img0002.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0001.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the FITC conjugate of NBP2-25196. Staining of CD19 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD19 antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0007.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the PE conjugate of NBP2-25196. Staining of CD19 in 1x10^6 human PBMC using 10 ul (0.25 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0008.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25196. Staining of human PBMC. Image from verified customer review.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0009.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the DyLight 405 conjugate of NBP2-25196. Staining of CD19 in human PBMCs using anti-CD19 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0010.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with TACI/TNFRSF13B/CVID (1A1) antibody NB600-1189PE and a matched isotype control. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0011.jpg)
![Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646.](http://images.novusbio.com/fullsize/CD19-Antibody-CB19-Flow-Cytometry-NBP2-25196-img0012.jpg)
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Cell surface analysis of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green: isotype control; red: anti-CD19 PE. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0011.jpg "Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Cell surface analysis of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green: isotype control; red: anti-CD19 PE. Image using the PE form of this antibody.")
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Cell surface analysis of CD19 in mouse splenocytes, 25 ug/10^6 cells. Anti-rat PE secondary and cell surface staining kit were used in this test.](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0001.jpg)
![Flow (Cell Surface): CD19 Antibody (1D3) [NBP2-24965] - Analysis using the Allophycocyanin conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents anti-CD19 APC.](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cell-Surface-NBP2-24965-img0006.jpg)
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Analysis using the Allophycocyanin conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes. Cells were stained with anti-CD4 PE this antibody and either 1 ug of isotype control antibody (left) or 1 ug of anti-CD19 APC (right).](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0007.jpg)
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Analysis using the PE conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes. Cells were stained with anti-CD4 FITC this antibody and either 1 ug of isotype control antibody (left, ) or 1 ug of anti-CD19 PE (right).](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0010.jpg)
![Western Blot: CD19 Antibody [NBP2-15782] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-Western-Blot-NBP2-15782-img0016.jpg "Western Blot: CD19 Antibody [NBP2-15782] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CD19 Antibody [NBP2-15782] - Human tonsil. CD19 stained by CD19 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD19-Antibody-Immunohistochemistry-Paraffin-NBP2-15782-img0011.jpg)
![Flow Cytometry: CD19 Antibody [NBP2-15782] - Mouse splenocytes cell fixed in 4% paraformaldehyde at 4-C for 15 min. Black: Unlabelled sample was used as a control. Red: CD19 antibody [C1C3] dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/CD19-Antibody-Flow-Cytometry-NBP2-15782-img0006.jpg)
![Western Blot: CD19 Antibody [NBP2-15782] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/CD19-Antibody-Western-Blot-NBP2-15782-img0007.jpg)
![Immunohistochemistry-Paraffin: CD19 Antibody [NBP2-15782] - Human tonsil. Green: CD19 stained by CD19 antibody [C1C3] diluted at 1:250.Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD19-Antibody-Immunohistochemistry-Paraffin-NBP2-15782-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody [NBP2-15782] -Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD19 stained by CD19 antibody [C1C3] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-15782_rabbit-polyclonal-cd19-antibody-immunocytochemistry-immunofluorescence-35202413518..jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: CD19 Antibody (JF100-06) [NBP2-67229] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunohistochemistry-Paraffin-NBP2-67229-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0002.jpg)
![Immunohistochemistry-Paraffin: CD19 Antibody (JF100-06) [NBP2-67229] - Analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunohistochemistry-Paraffin-NBP2-67229-img0003.jpg)
![Western Blot: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of CD19 on different lysates with Rabbit anti-CD19 antibody at 1/1,000 dilution.Lane 1: Raji cell lysateLane 2: Daudi cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 61 kDaObserved band size: 90 kDaExposure time: 2 minutes;8% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-western-blot-1382024114350..jpg)
![Flow Cytometry: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of Daudi cells labeling CD19.Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-flow-cytometry-138202411462..jpg)
![Flow Cytometry: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of Raji cells labeling CD19.Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-flow-cytometry-1382024114721..jpg)
![Western Blot: CD19 Antibody (LE-CD19) [NB100-65672] - analysis of Raji human Lymphoblastic Burkitt's lymphoma whole cell lysate](http://images.novusbio.com/fullsize/CD19-Antibody-LE-CD19-Western-Blot-NB100-65672-img0002.jpg "Western Blot: CD19 Antibody (LE-CD19) [NB100-65672] - analysis of Raji human Lymphoblastic Burkitt's lymphoma whole cell lysate")
![Immunohistochemistry: CD19 Antibody (LE-CD19) [NB100-65672] - Mouse anti human CD19 staining formalin-fixed, paraffin-embedded human tonsil.](http://images.novusbio.com/fullsize/CD19-Antibody-LE-CD19-Immunohistochemistry-NB100-65672-img0001.jpg)
![Immunohistochemistry-Frozen: CD19 Antibody (SJ25-C1) [NBP1-28375] - CD19 (red) stained in human skin, counterstained with DAPI (blue) at 20x. Tissue fixed with 4% PFA, permeabilized with 0.5% Triton, and blocked with 37% Egg DI, 5% Milk DI, and 3% FSG 2% BSA TBS Ca/Azide in that order. Anti-CD19 applied at 1:500 v/v, 4C, O/N. Alexa Fluor 568 secondary antibody, with DAPI counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/CD19-Antibody-SJ25-C1-Immunohistochemistry-Frozen-NBP1-28375-img0006.jpg "Immunohistochemistry-Frozen: CD19 Antibody (SJ25-C1) [NBP1-28375] - CD19 (red) stained in human skin, counterstained with DAPI (blue) at 20x. Tissue fixed with 4% PFA, permeabilized with 0.5% Triton, and blocked with 37% Egg DI, 5% Milk DI, and 3% FSG 2% BSA TBS Ca/Azide in that order. Anti-CD19 applied at 1:500 v/v, 4C, O/N. Alexa Fluor 568 secondary antibody, with DAPI counterstain. Image from verified customer review.")
![Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19-APC and Mouse Anti-Human CD3-FITC.](http://images.novusbio.com/fullsize/CD19-Antibody-SJ25-C1-Flow-Cytometry-NBP1-28375-img0007.jpg)
![Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2682024158261.jpg "Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds")
![Western Blot: CD19 Antibody (PSH03-94) [NBP3-32130] - Western blot analysis of CD19 on mouse spleen tissue lysates with Rabbit anti-CD19 antibody (NBP3-32130) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 60-120 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32130) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-278202415212455.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling CD19 with Rabbit anti-CD19 antibody (NBP3-32130) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32130, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-29820241420976.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue with Rabbit anti-CD19 antibody (NBP3-32130) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32130) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2982024149261.jpg)