Bim Antibodies
We offer Bim Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each Bim Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Bim Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Bim Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Bim polyclonal antibodies and browse our Bim monoclonal antibody catalog.
Alternate Names for Bim Antibodies
anti-Bim antibody, anti-BCL2L11 antibody, anti-BAM antibody, anti-bcl-2 interacting mediator of cell death antibody, anti-bcl-2 interacting protein Bim antibody, anti-bcl-2-like protein 11 antibody, anti-bcl-2-related ovarian death agonist antibody, anti-Bcl2-interacting mediator of cell death antibody, anti-bcl2-L-11 antibody, anti-BCL2-like 11 (apoptosis facilitator) antibody, anti-BIM-alpha6 antibody, anti-BIM-beta6 antibody, anti-BIM-beta7 antibody, anti-BIMBOD antibody, anti-BimEL antibody, anti-BimL antibody
15 Results for "bim" in Primary Antibodies
![Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0008.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in HeLa Cells. Loading: 15 ug of lysate per lane. Antibodies: Bim NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate. Bim antibody can detect all three human isoforms, including EL, L and S isoforms.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0009.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0010.jpg)
![Western Blot: Bim Antibody - BSA Free [NBP1-76963] - Figure 3 Western Blot Validation in Human TissueLoading: 15 u of lysates per lane.Antibodies: BIM , (0.5 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human thymusLane 2: Human colon](http://images.novusbio.com/fullsize/nbp1-76963_rabbit-polyclonal-bim-antibody-2711202420115554.gif)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-76963] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Bim with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76963_rabbit-polyclonal-bim-antibody-271120242045646.gif)
![Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP2-53088-img0001.jpg "Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP2-53088] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Bim at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53088-img0003.jpg)
![Immunohistochemistry-Paraffin: Bim Antibody [NBP2-53088] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human endometrial cancer xenograft using Bim antibody at 1:50 dilution. The signal was detected using HRP labeled secondary antibody and DAB staining method which followed counter-staining with hematoxylin. The antibody generated a specific cytoplasmic staining with punctate appearance, signifying Bim/Bcl2L11 presence on mitochondria.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunohistochemistry-Paraffin-NBP2-53088-img0002.jpg)
![Western Blot: Bim Antibody (SU0318) [NBP2-67456] - Analysis of Bim on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Western-Blot-NBP2-67456-img0006.jpg "Western Blot: Bim Antibody (SU0318) [NBP2-67456] - Analysis of Bim on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456] - Staining Bim in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Immunocytochemistry-Immunofluorescence-NBP2-67456-img0003.jpg)
![Immunohistochemistry-Paraffin: Bim Antibody (SU0318) [NBP2-67456] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Bim antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Immunohistochemistry-Paraffin-NBP2-67456-img0004.jpg)
![Western Blot: Bim Antibody (SU0318) [NBP2-67456] - Analysis of Bim on different lysates using anti-Bim antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Jurkat Lane 3: A431](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Western-Blot-NBP2-67456-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456] - Staining Bim in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Immunocytochemistry-Immunofluorescence-NBP2-67456-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456] - Staining Bim in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Immunocytochemistry-Immunofluorescence-NBP2-67456-img0002.jpg)
![Western Blot: Bim Antibody (10B12) - BSA Free [NBP2-80103] - Using Bim, mAb (10B12) (Prod. No. NBP2-80103)](http://images.novusbio.com/fullsize/Bim-Antibody-10B12-BSA-Free-Western-Blot-NBP2-80103-img0001.jpg "Western Blot: Bim Antibody (10B12) - BSA Free [NBP2-80103] - Using Bim, mAb (10B12) (Prod. No. NBP2-80103)")
![Bim Antibody (10B12) - BSA Free [NBP2-80103] - Data in permeabilized and fixed transfected cells using Bim, mAb (10B12) (Prod. No. NBP2-80103)](http://images.novusbio.com/fullsize/Bim-Antibody-10B12-BSA-Free-NBP2-80103-img0002.jpg)
![Western Blot: Bim Antibody (3C5) [NBP2-80102] - Detecting EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 mAb was used as a loading control for all blots. B) Western blot using anti-Bim, mAb (3C5) detecting endogenous Bim expression in mouse tissues but not in Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) and detection by ECL. Probing with an anti-Hsp-70 mAb was used as a loading control.](http://images.novusbio.com/fullsize/Bim-Antibody-3C5-Western-Blot-NBP2-80102-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Bim Antibody (3C5) [NBP2-80102] - Detecting EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 mAb was used as a loading control for all blots. B) Western blot using anti-Bim, mAb (3C5) detecting endogenous Bim expression in mouse tissues but not in Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) and detection by ECL. Probing with an anti-Hsp-70 mAb was used as a loading control.")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: Bim Antibody (3C5) [NBP2-80102] - Data in permeabilized and fixed transfected cells using anti-Bim, mAb (3C5) anti-EE (positive control) or secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.](http://images.novusbio.com/fullsize/Bim-Antibody-3C5-Flow-Cytometry-NBP2-80102-img0001.jpg)
![Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0007.jpg "Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas")
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-77118] - IAV via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963 (0.5 ug/ml), BIM NBP1-77118 (5 ug/ml), beta-actin (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling BIM with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-2711202419381793.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM K562 Cells. Immunohistochemical analysis of K562 cells using anti-BIM antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241933343.jpg)
![Western Blot: Bim Antibody - BSA Free [NBP1-77118] - Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: BIM , (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241928862.gif)
![Western Blot: Bim Antibody (1C2H4) [NBP1-75511] - Western blot analysis of 5 ng of Bim recombinant protein with Bim antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Bim-Antibody-1C2H4-Western-Blot-NBP1-75511-img0003.jpg "Western Blot: Bim Antibody (1C2H4) [NBP1-75511] - Western blot analysis of 5 ng of Bim recombinant protein with Bim antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] - Immunofluorescence of Bim in K562 cells with Bim antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-75511_mouse-monoclonal-bim-antibody-1c2h4-271120241932616.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] - Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-75511_mouse-monoclonal-bim-antibody-1c2h4-271120242022921.jpg)
![Western Blot: Bim Antibody [NB100-1440] - (0.5ug/ml) of K562lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NB100-1440-img0002.jpg "Western Blot: Bim Antibody [NB100-1440] - (0.5ug/ml) of K562lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Bim-Antibody-3R0T2-Western-Blot-NBP3-15867-img0001.jpg "Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Bim-Antibody-3R0T2-Western-Blot-NBP3-15867-img0002.jpg)