ATG9A Antibodies
We offer ATG9A Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each ATG9A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ATG9A Antibodies can be used in a variety of model species: Bovine, Chicken, Human, Mouse, Primate, Rat. Use the list below to choose the ATG9A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ATG9A polyclonal antibodies and browse our ATG9A monoclonal antibody catalog.
Alternate Names for ATG9A Antibodies
anti-ATG9A antibody, anti-APG9 autophagy 9-like 1 (S. cerevisiae) antibody, anti-APG9 autophagy 9-like 1 antibody, anti-APG9L1APG9-like 1 antibody, anti-ATG9 autophagy related 9 homolog A (S. cerevisiae) antibody, anti-autophagy 9-like 1 protein antibody, anti-autophagy-related protein 9A antibody, anti-FLJ22169 antibody, anti-mATG9 antibody, anti-MGD3208 antibody
7 Results for "atg9a" in Primary Antibodies
![Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. (1) siRNA ATG9A knockdown and (2) wildtype ATG9A.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Western-Blot-NB110-56893-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. (1) siRNA ATG9A knockdown and (2) wildtype ATG9A.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody [NB110-56893] - After stimulation with the NOD1 ligand, macrophages increased their expression of autophagy proteins Atg9, LC3 and IRGM. Alveolar macrophages (AMs), monocyte-derived macrophages (MDMs) and monocytes (MNs) were incubated in the presence of 5 ug/ml of Tri-DAP for 24 h. Cells were pre-incubated with Rip2/p38 inhibitor SB203580 (SB) for 30 min prior to Tri-DAP stimulation to block NOD1-mediated responses, as indicated. Atg9 and LC3 proteins were measured in the cytosolic fractions by western blot analysis. Image collected and cropped by CiteAb from the following publication (//bmcpulmmed.biomedcentral.com/articles/10.1186/1471-2466-14-152), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Western-Blot-NB110-56893-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody [NB110-56893] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-ATG9A at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-56893-img0008.jpg)
![Immunohistochemistry: ATG9A Antibody [NB110-56893] - Staining in mouse intestine.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunohistochemistry-NB110-56893-img0005.jpg)
![Flow Cytometry: ATG9A Antibody [NB110-56893] - An intracellular stain was performed on Hela Cells with NB110-56893 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).](http://images.novusbio.com/fullsize/ATG9A-Antibody-Flow-Cytometry-NB110-56893-img0007.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of chloroquine, spautin-1 & MG132 on ATG protein levels in A549 cells. (A) A549 cells were treated with 50 μM chloroquine, 10 μM spautin-1 or 5 μM MG132 in the presence or absence of 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3, mean±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415371977.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - CD133 promoted glucose uptake.A. Levels of glucose uptake in Huh-7con & Huh-7sh133 cells. Mean fluorescence intensity of 2-NBDG uptake was measured by FASC analysis after incubating for 6 h. B. Time course of 2-NBDG uptake in these two cell lines. Statistical significance indicated as *p<0.05; **p<0.01. C. CD133 or empty vector was transfected respectively into LM3 cells. Glucose uptake was measured at the indicated times upon the medium added with 100 µM 2-NBDG. *p<0.05. D. Detection of relative activities of signaling pathways in Huh-7con & Huh-7sh133 cells by Western blotting. con: Huh-7con cells; sh: Huh-7sh133 cells. E. Detection of autophagic genes in Huh-7con & Huh-7sh133 cells by Western blotting. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437259), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415382433.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of TGF beta 1 on ATG protein levels & LC3B lipidation in A549 cells. (A) A549 cells were treated with 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415384151.jpg)
![Immunohistochemistry-Paraffin: ATG9A Antibody (SR3028) [NBP3-27739] - Immunohistochemical analysis of paraffin-embedded human lung cancer](http://images.novusbio.com/fullsize/nbp3-27739_rabbit-atg9a-mab-sr3028-752024939136.jpg "Immunohistochemistry-Paraffin: ATG9A Antibody (SR3028) [NBP3-27739] - Immunohistochemical analysis of paraffin-embedded human lung cancer")
![Western Blot: ATG9A Antibody (SR3028) [NBP3-27739] - Western blot analysis of ATG9A expression in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27739_rabbit-atg9a-mab-sr3028-7520241015115.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG9A Antibody (SR3028) [NBP3-27739] - Immunofluorescent analysis of Hepg2 cells](http://images.novusbio.com/fullsize/nbp3-27739_rabbit-atg9a-mab-sr3028-75202410133477.jpg)
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody [NBP2-32477] - Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32477-img0008.jpg "Immunocytochemistry/Immunofluorescence: ATG9A Antibody [NBP2-32477] - Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.")
![Immunohistochemistry-Paraffin: ATG9A Antibody [NBP2-32477] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunohistochemistry-Paraffin-NBP2-32477-img0009.jpg)
![Immunoprecipitation: ATG9A Antibody - Azide and BSA Free [NBP3-26074] - ATG9A was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 uL NBP3-26074 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-26074, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 94 kDa](http://images.novusbio.com/fullsize/nbp3-26074_rabbit-atg9a-pab-azide-and-bsa-free-13620242234114.png "Immunoprecipitation: ATG9A Antibody - Azide and BSA Free [NBP3-26074] - ATG9A was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 uL NBP3-26074 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-26074, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 94 kDa")