ASH2L Antibodies
We offer ASH2L Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunoprecipitation (Negative), Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each ASH2L Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ASH2L Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the ASH2L Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ASH2L polyclonal antibodies and browse our ASH2L monoclonal antibody catalog.
Alternate Names for ASH2L Antibodies
anti-ASH2L antibody, anti-ash2 (absent, small, or homeotic)-like (Drosophila) antibody, anti-ASH2 antibody, anti-ASH2L1ash2 (absent, small, or homeotic, Drosophila, homolog)-like antibody, anti-ASH2L2ASH2-like protein antibody, anti-Bre2 antibody, anti-set1/Ash2 histone methyltransferase complex subunit ASH2 antibody
11 Results for "ash2l" in Primary Antibodies
![Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody NB600-250 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-250-img0015.jpg "Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody NB600-250 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-250] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ASH2used at a dilution of 1:1,000 ( 1ug/ml).Detection: DAB](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-NB600-250-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ASH2L Antibody [NB600-250] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASH2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-250-img0012.jpg)
![Western Blot: ASH2L Antibody (10B6U5) [NBP3-16505] - Analysis of extracts of various cell lines, using ASH2L Rabbit mAb (NBP3-16505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/ASH2L-Antibody-10B6U5-Western-Blot-NBP3-16505-img0007.jpg "Western Blot: ASH2L Antibody (10B6U5) [NBP3-16505] - Analysis of extracts of various cell lines, using ASH2L Rabbit mAb (NBP3-16505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunofluorescence analysis of U-2 OS cells using ASH2L Rabbit mAb (NBP3-16505) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-10B6U5-Immunocytochemistry-Immunofluorescence-NBP3-16505-img0006.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody (10B6U5) [NBP3-16505] - Mouse brain using ASH2L Rabbit mAb (NBP3-16505) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ASH2L-Antibody-10B6U5-Immunohistochemistry-Paraffin-NBP3-16505-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunofluorescence analysis of C6 cells using ASH2L Rabbit mAb (NBP3-16505) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-10B6U5-Immunocytochemistry-Immunofluorescence-NBP3-16505-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunofluorescence analysis of NIH-3T3 cells using ASH2L Rabbit mAb (NBP3-16505) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-10B6U5-Immunocytochemistry-Immunofluorescence-NBP3-16505-img0005.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody (10B6U5) [NBP3-16505] - Rat brain using ASH2L Rabbit mAb (NBP3-16505) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ASH2L-Antibody-10B6U5-Immunohistochemistry-Paraffin-NBP3-16505-img0001.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody (10B6U5) [NBP3-16505] - Human colon using ASH2L Rabbit mAb (NBP3-16505) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ASH2L-Antibody-10B6U5-Immunohistochemistry-Paraffin-NBP3-16505-img0002.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0006.jpg "Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of rectum cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Immunohistochemistry-NBP2-61685-img0004.jpg)
![Flow Cytometry: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of K562 cells using ASH2L mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Flow-Cytometry-NBP2-61685-img0002.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0005.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mouse mAb against K562 (1) and F9 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0007.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of colon cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Immunohistochemistry-NBP2-61685-img0003.jpg)
![ELISA: ASH2L Antibody (6F6B9) [NBP2-61685] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-ELISA-NBP2-61685-img0001.jpg)
![Western Blot: ASH2L Antibody [NBP1-88858] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP1-88858-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ASH2L Antibody [NBP1-88858] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ASH2L Antibody [NBP1-88858] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88858-img0008.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody [NBP1-88858] - Staining of human tonsil shows nuclear positivity in non-germinal center.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-Paraffin-NBP1-88858-img0007.jpg)
![Western Blot: ASH2L Antibody [NBP1-88858] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP1-88858-img0006.jpg)
![Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-281-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-281] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ASH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-Paraffin-NB600-281-img0013.jpg)
![Chromatin Immunoprecipitation: ASH2L Antibody [NB600-281] - ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-281 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products abeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Chromatin-Immunoprecipitation-NB600-281-img0009.jpg)
![Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Western-Blot-NBP2-61686-img0004.jpg "Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)")
![Immunohistochemistry: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis of paraffin-embedded rectum cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Immunohistochemistry-NBP2-61686-img0003.jpg)
![Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Western-Blot-NBP2-61686-img0005.jpg)
![Immunohistochemistry: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis of paraffin-embedded esophageal cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Immunohistochemistry-NBP2-61686-img0002.jpg)
![ELISA: ASH2L Antibody (6B10H10) [NBP2-61686] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-ELISA-NBP2-61686-img0001.jpg)
![Western Blot: ASH2L Antibody [NBP3-18639] - Figure 1. Western blot analysis using the antibody directed against ASH2L Figure 1A: Western blot was performed using the antibody against hASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on nuclear extracts from HeLa cells (upper panel: lane 1) and ectopically expressed FLAG-tagged ASH2L (ASH2L-F, upper panel: lane 2). The lower panel shows the same western blot with an anti-FLAG antibody. The molecular weight marker is shown on the left.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP3-18639-img0002.jpg "Western Blot: ASH2L Antibody [NBP3-18639] - Figure 1. Western blot analysis using the antibody directed against ASH2L Figure 1A: Western blot was performed using the antibody against hASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on nuclear extracts from HeLa cells (upper panel: lane 1) and ectopically expressed FLAG-tagged ASH2L (ASH2L-F, upper panel: lane 2). The lower panel shows the same western blot with an anti-FLAG antibody. The molecular weight marker is shown on the left.")
![Western Blot: ASH2L Antibody [NBP3-18639] - Figure 1. Western blot analysis using the antibody directed against ASH2L Figure 1B: Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 ug) with the antibody against ASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/ASH2L Antibody-Western Blot-NBP3-18639-img0001.jpg)
![Western Blot: ASH2L Antibody [NBP2-15488] - Sample (20 ug) A: HeLa nucleus 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP2-15488-img0001.jpg "Western Blot: ASH2L Antibody [NBP2-15488] - Sample (20 ug) A: HeLa nucleus 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: ASH2L Antibody [NBP3-18668] - Figure 1. Western blot analysis using the antibody directed against ASH2L Western blot was performed on HeLa nuclear extract (HeLa NE, 20 ug) using the antibody against ASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/ASH2L Antibody-Western Blot-NBP3-18668-img0001.jpg "Western Blot: ASH2L Antibody [NBP3-18668] - Figure 1. Western blot analysis using the antibody directed against ASH2L Western blot was performed on HeLa nuclear extract (HeLa NE, 20 ug) using the antibody against ASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.")
![Sandwich ELISA: ASH2L Antibody (4G7) [H00009070-M09] - Detection limit for recombinant GST tagged ASH2L is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ASH2L-Antibody-4G7-Sandwich-ELISA-H00009070-M09-img0001.jpg "Sandwich ELISA: ASH2L Antibody (4G7) [H00009070-M09] - Detection limit for recombinant GST tagged ASH2L is 0.1 ng/ml as a capture antibody.")