Aly Antibodies
We offer Aly Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Sandwich ELISA, Western Blot. Each Aly Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Aly Antibodies can be used in a variety of model species: Human, Mouse, Xenopus. Use the list below to choose the Aly Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Aly polyclonal antibodies and browse our Aly monoclonal antibody catalog.
Alternate Names for Aly Antibodies
anti-Aly antibody, anti-ALYREF antibody, anti-Ally of AML-1 and LEF-1 antibody, anti-ALY/REF antibody, anti-ALYBEFbZIP enhancing factor antibody, anti-bZIP-enhancing factor BEF antibody, anti-REF antibody, anti-THO complex 4 antibody, anti-THO complex subunit 4 antibody, anti-tho4 antibody, anti-Transcriptional coactivator Aly/REF antibody
8 Results for "aly" in Primary Antibodies
![Western Blot: Aly Antibody (JE36-45) [NBP3-32033] - Western blot analysis of Aly on different lysates with Rabbit anti-Aly antibody (NBP3-32033) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: SK-OV-3 cell lysate (20 ug/Lane) Lane 6: HL-60 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Neuro-2a cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: Mouse testis tissue lysate (40 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32033) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32033_rabbit-aly-mab-je36-45-278202416285215.jpg "Western Blot: Aly Antibody (JE36-45) [NBP3-32033] - Western blot analysis of Aly on different lysates with Rabbit anti-Aly antibody (NBP3-32033) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: SK-OV-3 cell lysate (20 ug/Lane) Lane 6: HL-60 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Neuro-2a cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: Mouse testis tissue lysate (40 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32033) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Aly Antibody (JE36-45) [NBP3-32033] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Aly antibody (NBP3-32033) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32033) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32033_rabbit-aly-mab-je36-45-29820241358555.jpg)
![Immunocytochemistry/ Immunofluorescence: Aly Antibody (JE36-45) [NBP3-32033] - Immunocytochemistry analysis of HepG2 cells labeling Aly with Rabbit anti-Aly antibody (NBP3-32033) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aly antibody (NBP3-32033) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32033_rabbit-aly-mab-je36-45-29202410334059.jpg)
![Flow Cytometry: Aly Antibody (JE36-45) [NBP3-32033] - Flow cytometric analysis of HepG2 cells labeling Aly. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32033, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32033_rabbit-aly-mab-je36-45-29202416372142.jpg)
![Immunohistochemistry-Paraffin: Aly Antibody [NBP1-33598] - Human esophageal cancer ALY antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Aly-Antibody-Immunohistochemistry-Paraffin-NBP1-33598-img0009.jpg "Immunohistochemistry-Paraffin: Aly Antibody [NBP1-33598] - Human esophageal cancer ALY antibody diluted at 1:500.")
![Western Blot: Aly Antibody [NBP1-33598] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aly-Antibody-Western-Blot-NBP1-33598-img0006.jpg)
![Immunohistochemistry-Paraffin: Aly Antibody [NBP1-33598] - Human cervical carcinoma: Paraffin-embedded human cervical carcinoma diluted at 1:500.](http://images.novusbio.com/fullsize/Aly-Antibody-Immunohistochemistry-Paraffin-NBP1-33598-img0008.jpg)
![Western Blot: Aly Antibody [NBP1-33598] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aly antibody (NBP1-33598) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp1-33598_rabbit-polyclonal-aly-antibody-91020242075973.jpg)
![Western Blot: Aly Antibody (11G5) [NB100-670] - La cell extract was separated by SDS-PAGE and probed with Monoclonal Anti-ALY (0.4 ug/mL, lane 1) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304, lane 1,2) and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/Aly-Antibody-11G5-Western-Blot-NB100-670-img0002.jpg "Western Blot: Aly Antibody (11G5) [NB100-670] - La cell extract was separated by SDS-PAGE and probed with Monoclonal Anti-ALY (0.4 ug/mL, lane 1) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304, lane 1,2) and a chemiluminescent substrate.")
![Western Blot: Aly Antibody (6I4T10) [NBP3-16372] - Western blot anTHOC4/AlyREFsis of extracts of various cell lines, using THOC4/AlyREF Rabbit mAb (NBP3-16372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Aly-Antibody-6I4T10-Western-Blot-NBP3-16372-img0004.jpg "Western Blot: Aly Antibody (6I4T10) [NBP3-16372] - Western blot anTHOC4/AlyREFsis of extracts of various cell lines, using THOC4/AlyREF Rabbit mAb (NBP3-16372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Aly Antibody (6I4T10) [NBP3-16372] - Immunohistochemistry of paraffin-embedded mouse brain using THOC4/AlyREF Rabbit mAb (NBP3-16372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aly-Antibody-6I4T10-Immunohistochemistry-Paraffin-NBP3-16372-img0003.jpg)
![Immunohistochemistry-Paraffin: Aly Antibody (6I4T10) [NBP3-16372] - Immunohistochemistry of paraffin-embedded rat brain using THOC4/AlyREF Rabbit mAb (NBP3-16372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aly-Antibody-6I4T10-Immunohistochemistry-Paraffin-NBP3-16372-img0001.jpg)
![Immunohistochemistry-Paraffin: Aly Antibody (6I4T10) [NBP3-16372] - Immunohistochemistry of paraffin-embedded human esophageal using THOC4/AlyREF Rabbit mAb (NBP3-16372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aly-Antibody-6I4T10-Immunohistochemistry-Paraffin-NBP3-16372-img0002.jpg)
![Western Blot: Aly Antibody (SR1439) [NBP3-22102] - Western blot analysis using NBP3-22102 in HEK293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22102_rabbit-aly-mab-sr1439-57202316494211.jpg "Western Blot: Aly Antibody (SR1439) [NBP3-22102] - Western blot analysis using NBP3-22102 in HEK293 cell lysate.")
![Western Blot: Aly Antibody (2G8) [H00010189-M03] - Analysis of ALYREF expression in Jurkat.](http://images.novusbio.com/fullsize/Aly-Antibody-2G8-Western-Blot-H00010189-M03-img0003.jpg "Western Blot: Aly Antibody (2G8) [H00010189-M03] - Analysis of ALYREF expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: Aly Antibody (2G8) [H00010189-M03] - Analysis of monoclonal antibody to ALYREF on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Aly-Antibody-2G8-Immunocytochemistry-Immunofluorescence-H00010189-M03-img0001.jpg)
![Sandwich ELISA: Aly Antibody (2G8) [H00010189-M03] - Detection limit for recombinant GST tagged ALYREF is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aly-Antibody-2G8-Sandwich-ELISA-H00010189-M03-img0002.jpg)
![Western Blot: Aly Antibody [NBP2-56154] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Aly-Antibody-Western-Blot-NBP2-56154-img0002.jpg "Western Blot: Aly Antibody [NBP2-56154] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: Aly Antibody [NBP2-56154] - Staining of human cell line U-2 OS shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/Aly-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56154-img0001.jpg)
![Western Blot: Aly Antibody [NBP3-10855] - Western blot analysis of Aly in Mouse Pancreas lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/Aly-Antibody-Western-Blot-NBP3-10855-img0001.jpg "Western Blot: Aly Antibody [NBP3-10855] - Western blot analysis of Aly in Mouse Pancreas lysates. Antibody dilution at 1ug/ml")