ALKBH5 Antibodies
We offer ALKBH5 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each ALKBH5 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ALKBH5 Antibodies can be used in a variety of model species: Human. Use the list below to choose the ALKBH5 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ALKBH5 polyclonal antibodies.
Alternate Names for ALKBH5 Antibodies
anti-ALKBH5 antibody, anti-ABH5OFOXD antibody, anti-alkB, alkylation repair homolog 5 (E. coli) antibody, anti-Alkylated DNA repair protein alkB homolog 5 antibody, anti-EC 1.14.11.- antibody, anti-FLJ20308 antibody, anti-OFOXD1 antibody, anti-oxoglutarate and iron-dependent oxygenase domain containing antibody, anti-probable alpha-ketoglutarate-dependent dioxygenase ABH5 antibody
7 Results for "alkbh5" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: ALKBH5 Antibody [NBP2-78817] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ALKBH5 Antibody NBP2-78817 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78817-img0001.jpg "Immunocytochemistry/Immunofluorescence: ALKBH5 Antibody [NBP2-78817] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ALKBH5 Antibody NBP2-78817 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Western-Blot-NBP1-82188-img0008.jpg "Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis in human cell line U-87 MG.")
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Immunohistochemical analysis of human breast cancer biopsies. Immunohistochemistry was performed on breast cancer biopsies using ALKBH5 and HIF-1a antibodies. Representative negative and positive staining (biopsies from patients #2 and #3, respectively) are shown. Scale bar: 500 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/11743/text/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0023.jpg)
![Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis of ALKBH5 in MDA-MB-231 human breast cancer subclones using anti-ALKBH5 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Western-Blot-NBP1-82188-img0007.jpg)
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human liver shows weak to moderate nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0015.jpg)
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0018.jpg)
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human kidney shows nuclear positivity.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0019.jpg)
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0021.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: ALKBH5 Antibody [NBP1-82188] - ZNF217 and ALKBH5 regulate NANOG and KLF4 expression via modulation of m6A levels. MCF-7 subclones were exposed to 20% or 1% O2 for 48 h and immunoblot assays were performed. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/11743/text/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Western-Blot-NBP1-82188-img0022.jpg)
![Western Blot: ALKBH5 Antibody [NBP1-82188] - ZNF217 & ALKBH5 regulate NANOG & KLF4 expression via modulation of m6A levels. J. MCF-7 subclones exposed to 20% or 1% O2 for 48 h & immunoblot assays performed. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82188_rabbit-polyclonal-alkbh5-antibody-31020241534366.jpg)
![Western Blot: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (NBP3-25357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25357_rabbit-alkbh5-mab-hl2061-azide-and-bsa-free-2120249435518.jpg "Western Blot: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (NBP3-25357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] - Wild-type (WT) and ALKBH5 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (NBP3-25357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-25357_rabbit-alkbh5-mab-hl2061-azide-and-bsa-free-2120249383164.jpg)
![Western Blot: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (NBP3-25357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25357_rabbit-alkbh5-mab-hl2061-azide-and-bsa-free-2120249415989.jpg)
![Western Blot: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (NBP3-25357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25357_rabbit-alkbh5-mab-hl2061-azide-and-bsa-free-2120249374993.jpg)
![Western Blot: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (NBP3-25357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25357_rabbit-alkbh5-mab-hl2061-azide-and-bsa-free-2120249413350.jpg)
![Western Blot: ALKBH5 Antibody [NBP2-92048] - Western blot analysis of extracts of various cell lines, using ALKBH5 antibody (NBP2-92048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Western-Blot-NBP2-92048-img0001.jpg "Western Blot: ALKBH5 Antibody [NBP2-92048] - Western blot analysis of extracts of various cell lines, using ALKBH5 antibody (NBP2-92048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: ALKBH5 Antibody [NBP2-92048] - Western blot analysis of extracts of various cell lines, using ALKBH5 antibody (NBP2-92048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ALKBH5 Antibody-Western Blot-NBP2-92048-img0002.jpg)
![Western Blot: ALKBH5 Antibody (PSH01-16) [NBP3-32027] - Western blot analysis of ALKBH5 on different lysates with Rabbit anti-ALKBH5 antibody (NBP3-32027) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: MCF7 cell lysate (20 ug/Lane) Lane 6: F9 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35~44 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32027) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32027_rabbit-alkbh5-mab-psh01-16-27820241514174.jpg "Western Blot: ALKBH5 Antibody (PSH01-16) [NBP3-32027] - Western blot analysis of ALKBH5 on different lysates with Rabbit anti-ALKBH5 antibody (NBP3-32027) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: MCF7 cell lysate (20 ug/Lane) Lane 6: F9 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35~44 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32027) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ALKBH5 Antibody (PSH01-16) [NBP3-32027] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ALKBH5 antibody (NBP3-32027) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32027) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32027_rabbit-alkbh5-mab-psh01-16-298202414333632.jpg)
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP3-27856] - Immunohistochemical analysis of paraffin-embedded human tonsil.](http://images.novusbio.com/fullsize/nbp3-27856_rabbit-alkbh5-pab-7520249522022.jpg "Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP3-27856] - Immunohistochemical analysis of paraffin-embedded human tonsil.")
![Western Blot: ALKBH5 Antibody [NBP3-27856] - Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4C over night](http://images.novusbio.com/fullsize/nbp3-27856_rabbit-alkbh5-pab-752024120310.jpg)