Ago2/eIF2C2 Antibodies
We offer Ago2/eIF2C2 Antibodies for use in common research applications: Chromatin Immunoprecipitation, Electron Microscopy, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, RNA Inhibition, Sandwich ELISA, Western Blot. Each Ago2/eIF2C2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Ago2/eIF2C2 Antibodies can be used in a variety of model species: Drosophila, Human, Rat. Use the list below to choose the Ago2/eIF2C2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Ago2/eIF2C2 polyclonal antibodies and browse our Ago2/eIF2C2 monoclonal antibody catalog.
Alternate Names for Ago2/eIF2C2 Antibodies
anti-Ago2/eIF2C2 antibody, anti-EIF2C2 antibody, anti-AGO2argonaute 2 antibody, anti-argonaute2 antibody, anti-EC 3.1.26.n1 antibody, anti-EC 3.1.26.n2 antibody, anti-eIF-2C 2 antibody, anti-eIF2C 2 antibody, anti-Eukaryotic translation initiation factor 2C 2 antibody, anti-eukaryotic translation initiation factor 2C, 2 antibody, anti-hAgo2 antibody, anti-MGC3183 antibody, anti-PAZ Piwi domain protein antibody, anti-PPD antibody, anti-protein argonaute-2 antibody, anti-Protein slicer antibody, anti-Q10 antibody
17 Results for "ago2-eif2c2" in Primary Antibodies
![Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Argonaute 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysate](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Western-Blot-NBP2-67121-img0009.jpg "Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Argonaute 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysate")
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunocytochemistry-Immunofluorescence-NBP2-67121-img0008.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Argo2/elF2C2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0012.jpg)
![Flow Cytometry: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Hela cells with Argonaute 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Flow-Cytometry-NBP2-67121-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunocytochemistry-Immunofluorescence-NBP2-67121-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunocytochemistry-Immunofluorescence-NBP2-67121-img0003.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded human kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0004.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded mouse kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0005.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded mouse stomach tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0006.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Ago2/elF2C2 antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0010.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-Argo/elF2C2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-JF0992-Immunohistochemistry-Paraffin-NBP2-67121-img0011.jpg)
![Western Blot: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] - Western blot analysis of extracts of various cell lines, using Ago2/eIF2C2 antibody (NBP3-15873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-10S10L5-Western-Blot-NBP3-15873-img0004.jpg "Western Blot: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] - Western blot analysis of extracts of various cell lines, using Ago2/eIF2C2 antibody (NBP3-15873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] - Rat brain using Argonaute-2 Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15873_rabbit-ago2-eif2c2-mab-10s10l5-immunohistochemistry-paraffin-135202494529..jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] - Mouse liver tissue using Argonaute-2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15873_rabbit-ago2-eif2c2-mab-10s10l5-immunohistochemistry-paraffin-135202494632..jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] - Rat kidney tissue using Argonaute-2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15873_rabbit-ago2-eif2c2-mab-10s10l5-immunohistochemistry-paraffin-135202494732..jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (10S10L5) [NBP3-15873] -Rat liver tissue using Argonaute-2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15873_rabbit-ago2-eif2c2-mab-10s10l5-immunohistochemistry-paraffin-135202494832..jpg)
![Western Blot: Ago2/eIF2C2 Antibody [NBP3-13024] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AGO2 antibody (NBP3-13024) diluted at 1:1000.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-Western-Blot-NBP3-13024-img0001.jpg "Western Blot: Ago2/eIF2C2 Antibody [NBP3-13024] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AGO2 antibody (NBP3-13024) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody [NBP3-13024] - AGO2 antibody detects AGO2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGO2 protein stained by AGO2 antibody (NBP3-13024) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13024-img0003.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody [NBP3-13024] - Ago2/eIF2C2 antibody detects Ago2/eIF2C2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Ago2/eIF2C2 antibody (NBP3-13024) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-Immunohistochemistry-Paraffin-NBP3-13024-img0002.jpg)
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - Immunochemical staining of AGO2 in human brain with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-036-Immunohistochemistry-Paraffin-NBP2-90675-img0002.jpg "Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - Immunochemical staining of AGO2 in human brain with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - Immunochemical staining of AGO2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-036-Immunohistochemistry-Paraffin-NBP2-90675-img0001.jpg)
![Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - AGO2 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-Mouse AGO2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse AGO2 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size: 100 kDa](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-036-Immunoprecipitation-NBP2-90675-img0004.jpg)
![Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - AGO2 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate. Lane B:0.5 mg MCF-7 Whole Cell Lysate. Lane C:0.5 mg HEK293 Whole Cell Lysate 4 uL anti-AGO2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AGO2 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size :100 kDa.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-036-Immunoprecipitation-NBP2-90675-img0005.jpg)
![Western Blot: Ago2/eIF2C2 [p Tyr393] Antibody [NBP3-23364] - Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2/eIF2C2 (NBP3-23228) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23364_rabbit-ago2-eif2c2-p-tyr393-pab-19920231410113.jpg "Western Blot: Ago2/eIF2C2 [p Tyr393] Antibody [NBP3-23364] - Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2/eIF2C2 (NBP3-23228) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).")
![Western Blot: Ago2/eIF2C2 Antibody [NBP3-23235] - Western blot analysis of mouse recombinant Ago2/eIF2C2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2/eIF2C2 (NBP3-23235) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Ser-387) phospho-specific antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23235_rabbit-ago2-eif2c2-pab-199202312523612.jpg "Western Blot: Ago2/eIF2C2 Antibody [NBP3-23235] - Western blot analysis of mouse recombinant Ago2/eIF2C2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2/eIF2C2 (NBP3-23235) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Ser-387) phospho-specific antibody (lanes 3 & 4).")
![Western Blot: Ago2/eIF2C2 [p Ser387] Antibody [NBP3-23236] - Western blot analysis of mouse recombinant Ago2/eIF2C2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2/eIF2C2 (NBP3-23235) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Ser-387) phospho-specific antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23236_rabbit-ago2-eif2c2-p-ser387-pab-19920231856925.jpg "Western Blot: Ago2/eIF2C2 [p Ser387] Antibody [NBP3-23236] - Western blot analysis of mouse recombinant Ago2/eIF2C2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2/eIF2C2 (NBP3-23235) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Ser-387) phospho-specific antibody (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (7C2) [NBP3-26520] - Staining of MCF-7 with Ago2/eIF2C2 Antibody (7C2) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 492-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26520_rabbit-ago2-eif2c2-mab-7c2-28220241323626.jpg "Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (7C2) [NBP3-26520] - Staining of MCF-7 with Ago2/eIF2C2 Antibody (7C2) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 492-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Ago2/eIF2C2 Antibody (7C2) [NBP3-26520] - Overlay Peak curve showing Hela cells stained with Ago2/eIF2C2 Antibody (7C2) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26520_rabbit-ago2-eif2c2-mab-7c2-282202414183698.jpg)
![Western Blot: Ago2/eIF2C2 Antibody (4D1-1D1) [H00027161-M02] - Detection against Immunogen (68.03 KDa) .](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-4D1-1D1-Western-Blot-H00027161-M02-img0003.jpg "Western Blot: Ago2/eIF2C2 Antibody (4D1-1D1) [H00027161-M02] - Detection against Immunogen (68.03 KDa) .")
![ELISA: Ago2/eIF2C2 Antibody (4D1-1D1) [H00027161-M02] - Detection limit for recombinant GST tagged EIF2C2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-4D1-1D1-Sandwich-ELISA-H00027161-M02-img0001.jpg)
![Western Blot: Ago2/eIF2C2 Antibody (11A9) [NBP3-23228] - Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2/eIF2C2 (NBP3-23228) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23228_rat-ago2-eif2c2-mab-11a9-199202312403711.jpg "Western Blot: Ago2/eIF2C2 Antibody (11A9) [NBP3-23228] - Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2/eIF2C2 (NBP3-23228) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody [NBP2-58382] - Staining of human cell line A-431 shows localization to nucleoplasm & cell junctions.Ab staining is shown in green](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58382-img0002.jpg "Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody [NBP2-58382] - Staining of human cell line A-431 shows localization to nucleoplasm & cell junctions.Ab staining is shown in green")
![Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody [NBP2-99647] - Immunofluorescence staining of Ago2/eIF2C2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Ago2/eIF2C2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/Ago2-eIF2C2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99647-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody [NBP2-99647] - Immunofluorescence staining of Ago2/eIF2C2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Ago2/eIF2C2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus .")