ADE2 Antibodies
We offer ADE2 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Simple Western, Western Blot. Each ADE2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ADE2 Antibodies can be used in a variety of model species: Human, Rat. Use the list below to choose the ADE2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ADE2 polyclonal antibodies and browse our ADE2 monoclonal antibody catalog.
Alternate Names for ADE2 Antibodies
anti-ADE2 antibody, anti-PAICS antibody, anti-AIR carboxylase antibody, anti-AIRCADE2H1 antibody, anti-DKFZp781N1372 antibody, anti-MGC1343 antibody, anti-MGC5024 antibody, anti-multifunctional protein ADE2 antibody, anti-PAIS antibody, anti-phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazolesuccinocarboxamide synthetase antibody, anti-SAICAR synthetase antibody
9 Results for "ade2" in Primary Antibodies
![Western Blot: ADE2 Antibody [NBP1-90235] - Analysis using Anti-PAICS antibody NBP1-90235 (A) shows similar pattern to independent antibody NBP2-58966 (B).](http://images.novusbio.com/fullsize/ADE2-Antibody-Western-Blot-NBP1-90235-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ADE2 Antibody [NBP1-90235] - Analysis using Anti-PAICS antibody NBP1-90235 (A) shows similar pattern to independent antibody NBP2-58966 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Analysis in human liver and skeletal muscle tissues using NBP1-90235 antibody. Corresponding PAICS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0015.jpg)
![Western Blot: ADE2 Antibody [NBP1-90235] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ADE2-Antibody-Western-Blot-NBP1-90235-img0008.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0010.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0011.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0012.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0013.jpg)
![Simple Western: ADE2 Antibody [NBP1-90235] - Simple Western lane view shows a specific band for ADE2 in 0.2 mg/ml of RT-4 and U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ADE2-Antibody-Simple-Western-NBP1-90235-img0005.jpg)
![Simple Western: ADE2 Antibody [NBP1-90235] - Electropherogram image(s) of corresponding Simple Western lane view. ADE2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/ADE2-Antibody-Simple-Western-NBP1-90235-img0006.jpg)
![Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Western-Blot-NBP2-02817-img0005.jpg "Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: ADE2 Antibody (OTI1B4) [NBP2-02817] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ADE2.](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Immunocytochemistry-Immunofluorescence-NBP2-02817-img0004.jpg)
![Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ADE2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Flow-Cytometry-NBP2-02817-img0002.jpg)
![Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADE2.](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Western-Blot-NBP2-02817-img0003.jpg)
![Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817] - Analysis of Jurkat cells, using anti-ADE2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Flow-Cytometry-NBP2-02817-img0001.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP2-15290] - Paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP2-15290-img0002.jpg "Immunohistochemistry-Paraffin: ADE2 Antibody [NBP2-15290] - Paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP2-15290] - Paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP2-15290-img0001.jpg)
![Western Blot: ADE2 Antibody [NBP2-15290] -Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAICS antibody [N1C1] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15290_rabbit-polyclonal-ade2-antibody-western-blot-811202494659..png)
![Western Blot: ADE2 Antibody [NBP3-38269] - Western blot analysis of various lysates using ADE2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38269_rabbit-ade2-pab-91220241581283.jpg "Western Blot: ADE2 Antibody [NBP3-38269] - Western blot analysis of various lysates using ADE2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: ADE2 Antibody [NBP3-38269] - Immunofluorescence analysis of U2OS cells using ADE2 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38269_rabbit-ade2-pab-91220241517528.jpg)
![Western Blot: ADE2 Antibody [NBP2-58966] - Analysis using Anti-PAICS antibody NBP2-58966 (A) shows similar pattern to independent antibody NBP1-90235 (B).](http://images.novusbio.com/fullsize/ADE2-Antibody-Western-Blot-NBP2-58966-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ADE2 Antibody [NBP2-58966] - Analysis using Anti-PAICS antibody NBP2-58966 (A) shows similar pattern to independent antibody NBP1-90235 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: ADE2 Antibody [NBP2-58966] - Staining of human cell line HEK 293 shows localization to cytosol.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58966-img0001.jpg)
![Western Blot: ADE2 Antibody [NBP2-15289] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ADE2-Antibody-Western-Blot-NBP2-15289-img0003.jpg "Western Blot: ADE2 Antibody [NBP2-15289] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP2-15289] - U87 xenograft, using PAICS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP2-15289-img0001.jpg)
![Immunohistochemistry:[NBP3-29140]-Detection of mouse ADE2 by immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Antibody: Affinity purified rabbit anti-ADE2 used at a dilution of 1: 1,000. Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29140_rabbit-ade2-pab-231202512101661.jpg "Immunohistochemistry:[NBP3-29140]-Detection of mouse ADE2 by immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Antibody: Affinity purified rabbit anti-ADE2 used at a dilution of 1: 1,000. Detection: DAB")
![Immunoprecipitation:ADE2 AntibodyNBP3-29511] -Detection of human ADE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADE2 antibody NBP3-29511 (lot NBP3-29511-1) used for IP at 6 µg per reaction. ADE2 was also immunoprecipitated by rabbit anti-ADE2.. For blotting immunoprecipitated ADE2, NBP3-29511 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29511_rabbit-ade2-pab-301220241735512.jpg "Immunoprecipitation:ADE2 AntibodyNBP3-29511] -Detection of human ADE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADE2 antibody NBP3-29511 (lot NBP3-29511-1) used for IP at 6 µg per reaction. ADE2 was also immunoprecipitated by rabbit anti-ADE2.. For blotting immunoprecipitated ADE2, NBP3-29511 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: ADE2 Antibody (4F4) [H00010606-M01] - PAICS monoclonal antibody (M01), clone 4F4. Analysis of PAICS expression in human placenta.](http://images.novusbio.com/fullsize/ADE2-Antibody-4F4-Western-Blot-H00010606-M01-img0002.jpg "Western Blot: ADE2 Antibody (4F4) [H00010606-M01] - PAICS monoclonal antibody (M01), clone 4F4. Analysis of PAICS expression in human placenta.")
![Sandwich ELISA: ADE2 Antibody (4F4) [H00010606-M01] - Detection limit for recombinant GST tagged PAICS is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ADE2-Antibody-4F4-Sandwich-ELISA-H00010606-M01-img0001.jpg)