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Regulation Of Translation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Regulation Of Translation Pathway and Malignant Neoplasms, Neoplasms, Malignant Paraganglionic Neoplasm, Neoplasm Metastasis, Physiological Stress. The study of the Regulation Of Translation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Regulation Of Translation Pathway has been researched in relation to Translation, Cell Growth, Transport, Cell Cycle, Localization. The Regulation Of Translation Pathway complements our catalog of research reagents including antibodies and ELISA kits against EIF2ALPHA, AKT1, EEF2, EIF2B1, EIF4A1.

Top Research Reagents

We have 2592 products for the study of the Regulation Of Translation Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

eEF-2 Antibody (4E2Z5)
NBP3-16866
Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Western blot analysis of extracts of various cell lines, using eEF-2 Rabbit mAb (NBP3-16866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Western blot analysis of extracts of Rat spleen, using eEF-2 Rabbit mAb (NBP3-16866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

Ribosomal Protein S6/RPS6 Antibody
NB100-1595
Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.PreviousImmunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

6 Publications
eIF2B4 Antibody
NBP3-24813
Analysis in human cell line RT-4 and human cell line U-251 MG.Staining of human cell line Hep-G2 shows localization to endoplasmic reticulum.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

TOR/mTOR [p Ser2448] Antibody
NB600-607
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - affinity purified TOR/TOR/mTOR [p Ser2448] antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ELISA, IHC

     2 Reviews

9 Publications
EIF2B2 Antibody
NBP3-38367
Immunocytochemistry/ Immunofluorescence: EIF2B2 Antibody [NBP3-38367] - Immunofluorescence analysis of C6 cells using EIF2B2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Immunohistochemistry: EIF2B2 Antibody [NBP3-38367] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using EIF2B2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

Tbp7 Antibody
NBP1-87799
Western Blot: Tbp7 Antibody [NBP1-87799] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.Immunocytochemistry/Immunofluorescence: Tbp7 Antibody [NBP1-87799] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
eIF2B1 Antibody
NBP2-16292
Western Blot: eIF2B1 Antibody [NBP2-16292] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 12% SDS PAGE gel, diluted at 1:10000.Immunocytochemistry/Immunofluorescence: eIF2B1 Antibody [NBP2-16292] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2B1 protein stained by EIF2B1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

eIF4A2 Antibody
NBP2-24529
Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Rabbit Polyclonal
Species Human, Mouse, Canine
Applications WB, Simple Western, IHC

     1 Review

1 Publication
eIF4A1 Antibody
NBP2-24632
Western Blot: eIF4A1 Antibody [NBP2-24632] - Representative immunoblot of total lysate of MEFs with eIF4A (C-terminal epitope) and Tubulin antibodies in control and GS (glucose starvation). Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/21907) licensed under a CC-BY license.Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

2 Publications
AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
AF887
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (<a class=Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

50 Publications
ERK2 Antibody [Unconjugated]
AF1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class= ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, KO

3 Publications
Insulin Antibody (182410) [Unconjugated]
MAB1417
Insulin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (<a class=

Rat Monoclonal
Species Human, Mouse, Bovine
Applications IHC, CyTOF-ready, ICC

25 Publications
TNF-alpha [Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

822 Publications
PABP Antibody (10E10) - BSA Free
NB120-6125
Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.Immunocytochemistry/Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.

Mouse Monoclonal
Species Human, Bovine, Canine
Applications WB, ELISA, Flow

     1 Review

11 Publications
eIF4G1 Antibody
NB100-268
Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: eIF4G1 Antibody [NB100-268] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for IP at 6 ug per reaction. eiF4G1/eiF4GI was also immunoprecipitated by rabbit antieiF4G1/eiF4GI antibody. For blotting immunoprecipitated eiF4G1/eiF4GI, rabbit antieiF4G1/eiF4GI antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Rabbit Polyclonal
Species Human, Porcine
Applications WB, IP

26 Publications
EIF2 beta Antibody (2F3)
H00008894-M09
Western Blot: EIF2 beta Antibody (2F3) [H00008894-M09] - Analysis of EIF2S2 expression in NIH/3T3 (Cat # L018V1).Immunocytochemistry/Immunofluorescence: EIF2 beta Antibody (2F3) [H00008894-M09] - Analysis of monoclonal antibody to EIF2S2 on HeLa cell. Antibody concentration 10 ug/ml

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

1 Publication
4EBP1 Antibody
NB200-157
Western Blot: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human 4EBP1 Polyclonal Antibody (Catalog # NB200-157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

45 Publications
eIF4E [p Ser209] Antibody
NB100-81898
Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] - Staining of methanol-fixed MCF cells using NB100-81898

Rabbit Polyclonal
Species Human, Porcine
Applications WB, ICC/IF, IHC

1 Publication