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Meiotic Prophase I Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Meiotic Prophase I Pathway and Infertility, Sterility, Male Infertility, Neoplasms, Polyploidy. The study of the Meiotic Prophase I Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Meiotic Prophase I Pathway has been researched in relation to Prophase, Meiosis, Pachytene, Zygotene, Spermatogenesis. The Meiotic Prophase I Pathway complements our catalog of research reagents including antibodies and ELISA kits against RAD51, ERMAP, SYCP3, SYCP1, SPO11.

Top Research Reagents

We have 2762 products for the study of the Meiotic Prophase I Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB300-229
Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) and SYCP1 (green), which are axial element and central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) and abnormal SYCP3 structures (arrows). Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. <i>PLoS One</i>  (2016)

Rabbit Polyclonal
Species Mouse, Rat, Chicken
Applications WB, Simple Western, ChIP

     3 Reviews

56 Publications
NB100-384
Detection of Human and Mouse Histone H2AX [p Ser139] by Western Blot. Samples: Nuclear extract (50 ug) from human HEK293, human melanoma (G361), mouse wildtype embryonic fibroblasts (+/+) or mouse H2AX knockout embryonic fibroblasts (-/-). Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody NB100-384 used at 0.1 ug/ml. Detection: Chemiluminescence with 30 second exposure. (NCS, neocarzinostatin - 200 ng/ml, 30 min). Bands appear at an observed molecular weight of ~15 kDa.Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right) . Antibody: Affinity purified rabbit Histone H2AX [p Ser139] used at a dilution of 1:5,000  (0.2ug/ml).  Detection: Red fluorescent Anti-rabbit IgG-DyLight 594 used at a dilution of 1:100.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     1 Review

161 Publications
NB100-204
Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DABImmunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] -  FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ELISA

36 Publications
NB100-181
Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.

Mouse Monoclonal
Species Human, Mouse
Applications WB, IP

     1 Review

1 Publication
NB100-56648
Western Blot: RISC Antibody [NB100-56648] - Analysis of human RISC in 15 ugs of human heart cell lysate using NB100-56648 at 1:1000 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB

NBP1-58172
Western Blot: SPO11 Antibody [NBP1-58172] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/mlWestern Blot: SPO11 Antibody [NBP1-58172] - HepG2 cell lysate, concentration 0.2-1 ug/ml.

Rabbit Polyclonal
Species Human, Mouse
Applications WB

1 Publication
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     3 Reviews

42 Publications
NBP1-87145
Immunocytochemistry/Immunofluorescence: REC8 Antibody [NBP1-87145] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.Immunohistochemistry-Paraffin: REC8 Antibody [NBP1-87145] - Staining of human skin shows very weak positivity in squamous epthelial cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NBP2-02667
Western Blot: HID1 Antibody (OTI2F4) [NBP2-02667] - Analysis of extracts (10ug) from 10 Human tissue by using anti-C17orf28 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).Immunocytochemistry/Immunofluorescence: HID1 Antibody (2F4) [NBP2-02667] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY C17orf28.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

3 Publications
NBP2-03456
Western Blot: CTDSP1 Antibody (OTI1B6) [NBP2-03456] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTDSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTDSP1.Immunohistochemistry-Paraffin: CTDSP1 Antibody (OTI1B6) [NBP2-03456] - Staining of paraffin-embedded Human prostate tissue using anti-CTDSP1 mouse monoclonal antibody.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

NBP2-20053
Western Blot: Rad21 Antibody [NBP2-20053] - A: NIH-3T3  B: JC   C: BCL-1  7.5% SDS PAGE   diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: Rad21 Antibody [NBP2-20053] -  HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad21 stained by Rad21 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

AF4928
K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human ERMAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4928, filled histogram) or control antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, Flow, CyTOF-ready

NBP2-38851
Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining of human testis using Anti-STAG3 antibody NBP2-38851.Immunohistochemistry-Paraffin: STAG3 Antibody [NBP2-38851] - Staining of human testis shows high expression.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NB500-106
Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 100X objective and digitally deconvolved.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     5 Reviews

45 Publications
NBP2-67381
Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-RabbImmunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NBP1-20903
Immunocytochemistry/Immunofluorescence: CTDSPL Antibody [NBP1-20903] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Flow Cytometry: CTDSPL Antibody [NBP1-20903] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Goat Polyclonal
Species Human
Applications Flow, ICC/IF, PEP-ELISA

1 Publication
NB100-148
Western Blot: Rad51 Antibody (14B4) [NB100-148] - eIF3f facilitates hMSH4 stabilization. Western blotting analysis of the levels of HDAC3, hRad51, and VBP1 expression in 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells. 293T/eIF3f-hMSH4 cells treated with 1 or 10 Gy IR were fractionated at 6 hrs post-treatment and the levels of hMSH4 and eIF3f in the nuclear and cytoplasmic fractions were determined by immunoblotting. Alpha-tubulin was used as a marker for the cytoplasmic fraction. Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-51) licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - HCC1937 (human immortalized breast cancer) cells treated with PARPi for 96 h. Nuclei are stained in blue (DAPI) and RAD51 in red. ICC/IF image submitted by a verified customer review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     5 Reviews

65 Publications
NB100-207
Western Blot: SMC3 Antibody [NB100-207] - Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SMC3 antibody  used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 3 seconds.Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] -  Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SMC3  used at 1:5,000 (0.2ug/ml). Detection: DAB

Rabbit Polyclonal
Species Human, Mouse, C. elegans
Applications WB, IHC, IHC-P

9 Publications
NB300-232
Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Chromosome spread for sycp3.  ICC/IF image submitted by a verified customer review.Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC analysis of a formalin fixed and paraffin embedded tissue section of Mouse testis using SCP3 antibody at 1:1000 dilution. The signal was detected using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated a specific signal of SCP3 in the spermatogonial cells and the spermatocytes. The signal was strongest in the nuclei of spermatogonial cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IB, ICC/IF

     2 Reviews

82 Publications