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Freezing Tolerance Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Freezing Tolerance Pathway and Dehydration, Physiological Stress, Dwarfism, Stress, Water, Malnutrition. The study of the Freezing Tolerance Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Freezing Tolerance Pathway has been researched in relation to Cold Acclimation, Germination, Photosynthesis, Response To Cold, Transport. The Freezing Tolerance Pathway complements our catalog of research reagents including antibodies and ELISA kits against FT, GUS, C2, CALCR, CALR.

Top Research Reagents

We have 1987 products for the study of the Freezing Tolerance Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB600-302
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY license.Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). <br/>Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Mouse Monoclonal
Species Human, Mouse, Bovine
Applications WB, Simple Western, ELISA

     4 Reviews

46 Publications
NB600-101
Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, DB

28 Publications
NB100-56565
Simple Western lane view shows a specific band for Caspase 1 in 1.0 mg/ml of HeLa lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.Activity of Casp1 in OHT-injured and normotensive control eyes. (A) Casp1 was detected by intraocular injection FLICA660-labeled substrate (green) in vivo 24 h after injury. Bright labeling (arrows) is evident in cells in the GCL and inner nuclear layer (INL) layers of the OHT-challenged retinas, a diffuse labeling of cell processes located in the IPL. Casp1 activity is diminished in Panx1-/- (Px1-/- OHT) retinas and WT retinas treated with probenecid (WT/Pbcd) at 12 h postinjury. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fnmol.2019.00036/full), licensed under a CC-BY license.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

72 Publications
NBP1-31113
Western Blot: eRF1 Antibody [NBP1-31113] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eRF1 antibody diluted at 1:500.Immunocytochemistry/Immunofluorescence: eRF1 Antibody [NBP1-31113] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eRF1 protein stained by eRF1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

2 Publications
NBP1-33427
Western Blot: csl/RBPJK Antibody [NBP1-33427] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with csl/RBPJK antibody [C1C3] (NBP1-33427) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody  was used to detect the primary antibody,Immunocytochemistry/Immunofluorescence: csl/RBPJK Antibody [NBP1-33427] -  Analysis of paraformaldehyde-fixed HeLa at 1:100 dilution.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, ChIP

     1 Review

1 Publication
NBP1-76639
Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 ug/ml.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ELISA, ICC/IF

7 Publications
NBP1-81838
Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

1 Publication
NBP1-84556
Western Blot: CES2 Antibody [NBP1-84556] - Analysis in human small intestine tissue.Immunocytochemistry/Immunofluorescence: CES2 Antibody [NBP1-84556] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
NBP2-04017
Western Blot: GTF3A Antibody [NBP2-04017] - Whole cell lysate (15 and 50 ug) from Jurkat cells.  Antibody used at 0.4 ug/ml.

Rabbit Polyclonal
Species Human
Applications WB, IP

NBP2-11567
Western Blot: SLC6A8 Antibody [NBP2-11567] - Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/ml, 1 hr incubation. Detected by chemiluminescence.Immunohistochemistry-Paraffin: SLC6A8 Antibody [NBP2-11567] - Human kidney. Antibody at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Goat Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

1 Publication
NBP2-16366
Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody (GTX114971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: ERF Antibody [NBP2-16366] -  HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERF stained by ERF antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10um.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

AF1443
Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Mouse, Rat
Applications WB, Simple Western, IHC

     2 Reviews

40 Publications
MAB4614
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # <a class=Calcitonin R knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications Flow, CyTOF-ready, KO

6 Publications
AF1936

Goat Polyclonal
Species Human
Applications WB, IP

1 Publication
664-LI
<P align=left>Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED<SUB>50</SUB> is 1-4 ng/mL.</P><p align=


Species Human
Applications BA

21 Publications
211-TBB/CF
Recombinant Human Lymphotoxin-alpha /TNF-beta  (Catalog # 211-TBB/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 4‑20 pg/mL.1 µg/lane of Recombinant Human Lymphotoxin-alpha /TNF-beta  was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 19 kDa.


Species Human
Applications BA

8 Publications
NBP2-47525
Western Blot: CBF2 Antibody [NBP2-47525] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10<br/>Lane 2: Human cell line NTERA-2Immunohistochemistry: CBF2 Antibody [NBP2-47525] - Analysis of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

     2 Reviews

NBP2-92856
Western Blot: ACBP Antibody [NBP2-92856] - Analysis of extracts of various cell lines, using DBI antibody  at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.Immunocytochemistry/Immunofluorescence: ACBP Antibody [NBP2-92856] - Immunofluorescence analysis of U2OS cells using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC