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![Western Blot: CBF2 Antibody [NBP2-47525] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line NTERA-2](http://images.novusbio.com/fullsize/CBF2-Antibody-Western-Blot-NBP2-47525-img0002.jpg "Western Blot: CBF2 Antibody [NBP2-47525] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line NTERA-2")
![Immunohistochemistry: CBF2 Antibody [NBP2-47525] - Analysis of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/CBF2-Antibody-Immunohistochemistry-NBP2-47525-img0001.jpg)
![Western Blot: CBF2 Antibody [NBP1-71909] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-C/EBP zeta antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C/EBP zeta was also immunoprecipitated by rabbit anti-C/EBP zeta antibodies NBP1-71908, NBP1-71910 and NBP1-71911, which recognize other epitopes.](http://images.novusbio.com/fullsize/CBF2-Antibody-Western-Blot-NBP1-71909-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CBF2 Antibody [NBP1-71909] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-C/EBP zeta antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C/EBP zeta was also immunoprecipitated by rabbit anti-C/EBP zeta antibodies NBP1-71908, NBP1-71910 and NBP1-71911, which recognize other epitopes.")
![Western Blot: CBF2 Antibody [NBP1-71911] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-C/EBP zeta antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C/EBP zeta was also immunoprecipitated by rabbit anti-C/EBP zeta antibodies NBP1-71908, NBP1-71909 and NBP1-71910, which recognize upstream epitopes.](http://images.novusbio.com/fullsize/CBF2-Antibody-Western-Blot-NBP1-71911-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CBF2 Antibody [NBP1-71911] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-C/EBP zeta antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C/EBP zeta was also immunoprecipitated by rabbit anti-C/EBP zeta antibodies NBP1-71908, NBP1-71909 and NBP1-71910, which recognize upstream epitopes.")
![Western Blot: CBF2 Antibody [NBP1-71910] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-C/EBP zeta antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C/EBP zeta was also immunoprecipitated by rabbit anti-C/EBP zeta antibodies NBP1-71908 ,NBP1-71909 and NBP1-71911, which recognize other epitopes.](http://images.novusbio.com/fullsize/CBF2-Antibody-Western-Blot-NBP1-71910-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CBF2 Antibody [NBP1-71910] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-C/EBP zeta antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C/EBP zeta was also immunoprecipitated by rabbit anti-C/EBP zeta antibodies NBP1-71908 ,NBP1-71909 and NBP1-71911, which recognize other epitopes.")
![Western Blot: CBF2 Antibody [NBP1-71908] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-C/EBP zeta antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C/EBP zeta was also immunoprecipitated by rabbit anti-C/EBP zeta antibodies NBP1-71909, NBP1-71910 and NBP1-71911, which recognize downstream epitopes.](http://images.novusbio.com/fullsize/CBF2-Antibody-Western-Blot-NBP1-71908-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CBF2 Antibody [NBP1-71908] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-C/EBP zeta antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C/EBP zeta was also immunoprecipitated by rabbit anti-C/EBP zeta antibodies NBP1-71909, NBP1-71910 and NBP1-71911, which recognize downstream epitopes.")
![Western Blot: CBF2 Antibody [NBP2-97347] - Anti-CBF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/CBF2-Antibody-Western-Blot-NBP2-97347-img0002.jpg "Western Blot: CBF2 Antibody [NBP2-97347] - Anti-CBF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 35 kDa")