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![Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NB600-1031-img0003.jpg "Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody [NB600-1031] - Analysis of human testis using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-Immunohistochemistry-Paraffin-NB600-1031-img0002.jpg)
![Knockdown Validated: UNG Antibody [NBP1-49985] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and UNG knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human UNG Polyclonal Antibody (Catalog # NBP1-49985) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UNG at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/UNG-Antibody-Knockdown-Validated-NBP1-49985-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: UNG Antibody [NBP1-49985] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and UNG knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human UNG Polyclonal Antibody (Catalog # NBP1-49985) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UNG at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: UNG Antibody [NBP1-49985] - Detection of Human UNG by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNG antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. UNG was successfully immunoprecipitated by rabbit anti-UNG antibody which recognizes an upstream epitope. For blotting immunoprecipitated UNG was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NBP1-49985-img0002.jpg)
![Western Blot: uracil-DNA glycosylase Antibody (1A11) [NBP2-01194] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY uracil-DNA glycosylase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-uracil-DNA glycosylase.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-1A11-Western-Blot-NBP2-01194-img0001.jpg "Western Blot: uracil-DNA glycosylase Antibody (1A11) [NBP2-01194] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY uracil-DNA glycosylase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-uracil-DNA glycosylase.")
![Immunocytochemistry/Immunofluorescence: uracil-DNA glycosylase Antibody (1A11) [NBP2-01194] Staining of COS7 cells transiently transfected by pCMV6-ENTRY uracil-DNA glycosylase.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-1A11-Immunocytochemistry-Immunofluorescence-NBP2-01194-img0007.jpg)
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody (1A11) [NBP2-01194] - Staining of paraffin-embedded Human tonsil using anti-uracil-DNA glycosylase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-01194-img0006.jpg)
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody (1A11) [NBP2-01194] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-uracil-DNA glycosylase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-01194-img0002.jpg)
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody (1A11) [NBP2-01194] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-uracil-DNA glycosylase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-01194-img0003.jpg)
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody (1A11) [NBP2-01194] - Staining of paraffin-embedded Carcinoma of Human luracil-DNA glycosylase tissue using anti-uracil-DNA glycosylase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-01194-img0004.jpg)
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody (1A11) [NBP2-01194] - Staining of paraffin-embedded Human lymphoma tissue using anti-uracil-DNA glycosylase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-1A11-Immunohistochemistry-Paraffin-NBP2-01194-img0005.jpg)
![Western Blot: UNG Antibody [NBP2-93120] - Analysis of extracts of various cell lines, using UNG at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NBP2-93120-img0003.jpg "Western Blot: UNG Antibody [NBP2-93120] - Analysis of extracts of various cell lines, using UNG at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Immunohistochemistry-Paraffin: UNG Antibody [NBP2-93120] - Paraffin-embedded mouse brain using UNG .](http://images.novusbio.com/fullsize/UNG-Antibody-Immunohistochemistry-Paraffin-NBP2-93120-img0002.jpg)
![Immunohistochemistry-Paraffin: UNG Antibody [NBP2-93120] - Paraffin-embedded human stomach using UNG .](http://images.novusbio.com/fullsize/UNG-Antibody-Immunohistochemistry-Paraffin-NBP2-93120-img0001.jpg)
![Western Blot: UNG Antibody [NBP2-94498] - Analysis of extracts of various cell lines, using UNG at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NBP2-94498-img0002.jpg "Western Blot: UNG Antibody [NBP2-94498] - Analysis of extracts of various cell lines, using UNG at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: UNG Antibody [NBP2-94498] - Analysis of HeLa cells using UNG at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/UNG-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94498-img0001.jpg)
![Western Blot: UNG Antibody (SR1540) [NBP3-22206] - Western blot analysis using NBP3-22206 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22206_rabbit-ung-mab-sr1540-672023106248.jpg "Western Blot: UNG Antibody (SR1540) [NBP3-22206] - Western blot analysis using NBP3-22206 in HeLa cell lysate.")
![Immunocytochemistry/Immunofluorescence: UNG Antibody [NBP2-98838] - Immunofluorescence staining of UNG in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human UNG polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/UNG-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98838-img0001.jpg "Immunocytochemistry/Immunofluorescence: UNG Antibody [NBP2-98838] - Immunofluorescence staining of UNG in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human UNG polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Immunohistochemistry-Paraffin: UNG Antibody [NBP2-98838] - Immunochemical staining of human UNG in human testis with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/UNG-Antibody-Immunohistochemistry-Paraffin-NBP2-98838-img0003.jpg)
![Immunohistochemistry-Paraffin: UNG Antibody [NBP2-98838] - Immunochemical staining of human UNG in human stomach with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/UNG-Antibody-Immunohistochemistry-Paraffin-NBP2-98838-img0002.jpg)
![Western Blot: UNG Antibody [NBP2-82371] - Host: Rabbit. Target Name: UNG. Sample Tissue: Human 293T Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NBP2-82371-img0001.jpg "Western Blot: UNG Antibody [NBP2-82371] - Host: Rabbit. Target Name: UNG. Sample Tissue: Human 293T Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: UNG Antibody [NBP2-82370] - WB Suggested Anti-UNG Antibody. Titration: 1.0 ug/ml. Positive Control: Hela Whole CellUNG is supported by BioGPS gene expression data to be expressed in HeLa](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NBP2-82370-img0001.jpg "Western Blot: UNG Antibody [NBP2-82370] - WB Suggested Anti-UNG Antibody. Titration: 1.0 ug/ml. Positive Control: Hela Whole CellUNG is supported by BioGPS gene expression data to be expressed in HeLa")
![Western Blot: uracil-DNA glycosylase Overexpression Lysate (Adult Normal) [NBL1-17621] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for uracil-DNA glycosylase.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17621-img0002.jpg "Western Blot: uracil-DNA glycosylase Overexpression Lysate (Adult Normal) [NBL1-17621] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for uracil-DNA glycosylase.")
![Western Blot: uracil-DNA glycosylase Overexpression Lysate (Adult Normal) [NBL1-17620] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for uracil-DNA glycosylase.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17620-img0002.jpg "Western Blot: uracil-DNA glycosylase Overexpression Lysate (Adult Normal) [NBL1-17620] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for uracil-DNA glycosylase.")