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![Western Blot: TRPA1 Antibody [NB110-40763] - Expression and localization of TRPA1 channels in wild-type (WT) and APP/PS1 Tg mice. Brains were harvested from WT and APP/PS1 Tg mice at 8A months old. Western blot analysis of protein levels of TRPA1 and I+--tubulin. Image collected and cropped by Citeab from the following publication (Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease. J Neuroinflammation (2016)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Western-Blot-NB110-40763-img0014.jpg "Western Blot: TRPA1 Antibody [NB110-40763] - Expression and localization of TRPA1 channels in wild-type (WT) and APP/PS1 Tg mice. Brains were harvested from WT and APP/PS1 Tg mice at 8A months old. Western blot analysis of protein levels of TRPA1 and I+--tubulin. Image collected and cropped by Citeab from the following publication (Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease. J Neuroinflammation (2016)) licensed under a CC-BY license.")
![Immunohistochemistry: TRPA1 Antibody [NB110-40763] - Staining TRPA1 in mouse intestine.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Immunohistochemistry-NB110-40763-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: TRPA1 Antibody [NB110-40763] - Ai2 elicits TRPA1-dependent Ca2+ influx in astrocytes. Immunostaining of primary astrocytes from WT mice with anti-IgG and anti-TRPA1 antibody, then FITC-conjugated secondary antibody. Image collected and cropped by Citeab from the following publication (Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease. J Neuroinflammation (2016)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40763-img0011.jpg)
![Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Flow-Cytometry-NB110-40763-img0010.jpg)
![Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488](http://images.novusbio.com/fullsize/TRPA1-Antibody-Flow-Cytometry-NB110-40763-img0009.jpg)
![Western Blot: TRPA1 Antibody [NB110-40763] - The essential role of TRPA1 in Ai2-induced calcium influx in HEK293 cells. (a) Western blot analysis of TRPA1 and I+--tubulin protein levels in non-treated, vector-or TRPA1-transfected HEK293 cells. Image collected and cropped by Citeab from the following publication (Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease. J Neuroinflammation (2016)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Western-Blot-NB110-40763-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: TRPA1 Antibody [NB110-40763] - Analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40763-img0006.jpg)
![Western Blot: TRPA1 Antibody - BSA Free [NB110-40763] - Effects of Lycium barbarum polysaccharides (LBP) & capsaicin (CAP) on colonic (A) Cyclooxygenase-2 (COX-2), (B) Transient receptor potential cation channel V1 (TRPV1), & (C) Transient receptor potential ankyrin 1 (TRPA1) protein expression. (D) Representatives of Western blot for COX-2, TRPV1, TRPA1, & beta -actin. N: control group, U: ulcerative colitis induced group, L: LBP treated group, C: CAP treated group, M: mixed LBP & CAP treated group. Data are presented as mean ± SEM & analyzed by one-way ANOVA & Fisher’s least significant difference test (n = 8). # p < 0.05 compared to the N group. * p < 0.05 compared to the U group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35269566), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40763_rabbit-polyclonal-trpa1-antibody-310202415392558.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody - BSA Free [NB110-40763] - Expression & localization of TRPA1 channels in wild-type (WT) & APP/PS1 Tg mice. (a) Brains were harvested from WT & APP/PS1 Tg mice at 8 months old. Western blot analysis of protein levels of TRPA1 & alpha -tubulin. Data are mean ± SEM from 6 mice in each group. *, P < 0.05 vs. WT mice. (b, c) Immunohistochemistry of specimens of cortex & hippocampus from 8-month-old WT & APP/PS1 Tg mice with the antibodies anti-TRPA1, anti-vWF (endothelial cell marker), anti-NeuN (neuron marker), anti-GFAP (astrocyte marker) & anti-IBA-1 (microglia marker), then FITC- or Texas red-conjugated secondary antibody. Bar = 50 μm. vWF-positive cells denoted endothelial cells, NeuN-positive cells denoted neurons, & GFAP-positive cells denoted astrocytes, as indicated by arrowheads, stars or arrows, respectively Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0557-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40763_rabbit-polyclonal-trpa1-antibody-310202416235534.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody - BSA Free [NB110-40763] - Evaluation of anti-TRPA1 antibody specificity by western blotting & immunofluorescence. (a) Lysates derived from HEK293T cells transiently transfected with the TRPA1 & GFP expression construct (TRPA1 OE) or TRPM2 & GFP negative control (TRPM2 OE) were probed with the antibodies indicated. Additional untransfected & GFP only controls are presented in the first blot (6G8). Uncropped full length blots are displayed, gaps between the blots are present to indicate the use of a different antibody. Mouse mAbs C-5 & 6G8, & NB110-40763 can detect TRPA1 only in the membrane fraction of lysates at the expected molecular weight (127.5 kDa). Several TRPA1-specific bands are observed above this weight. NB110-40763 also detects several other antigens. Ab58844 & ACC- 037 only appear to detect antigens other than TRPA1 in the conditions used. (b) The performance of the same antibodies was evaluated by immunofluorescence (red) in comparison to the expression of the GFP reporter (green), or control TRPM2 & GFP overexpressing cells as for western blotting. Mouse mAb C-5 again shows high specificity with a strong correlation between the expression of the GFP reporter & antibody staining, & no staining in TRPM2 & GFP overexpressing controls. NB110-40763 also demonstrates some sensitivity but has high background that likely does not correspond to Fc binding of the antibody, as the other two rabbit polyclonal antibodies or isotype control did show different staining patterns. Ab58844 & ACC- 037 only appear to detect antigens other than TRPA1 in the conditions used. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31811235), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40763_rabbit-polyclonal-trpa1-antibody-31020241623556.jpg)
![Western Blot: TRPA1 Antibody - BSA Free [NB110-40763] - Expression & localization of TRPA1 channels in wild-type (WT) & APP/PS1 Tg mice. (a) Brains were harvested from WT & APP/PS1 Tg mice at 8 months old. Western blot analysis of protein levels of TRPA1 & alpha -tubulin. Data are mean ± SEM from 6 mice in each group. *, P < 0.05 vs. WT mice. (b, c) Immunohistochemistry of specimens of cortex & hippocampus from 8-month-old WT & APP/PS1 Tg mice with the antibodies anti-TRPA1, anti-vWF (endothelial cell marker), anti-NeuN (neuron marker), anti-GFAP (astrocyte marker) & anti-IBA-1 (microglia marker), then FITC- or Texas red-conjugated secondary antibody. Bar = 50 μm. vWF-positive cells denoted endothelial cells, NeuN-positive cells denoted neurons, & GFAP-positive cells denoted astrocytes, as indicated by arrowheads, stars or arrows, respectively Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0557-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40763_rabbit-polyclonal-trpa1-antibody-310202416235536.jpg)
![Western Blot: TRPA1 Antibody [NB100-91319] - Tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:6000 incubated overnight at 4C.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Western-Blot-NB100-91319-img0001.jpg "Western Blot: TRPA1 Antibody [NB100-91319] - Tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:6000 incubated overnight at 4C.")
![Western Blot: TRPA1 Antibody [NB100-91319] - TRPA1 inhibitor HC030031 significantly ameliorated the oxidative stress & apoptosis levels of Pg.LPS-induced PDLCs. (a) P.g.LPS increased intracellular calcium ion level in a dose-dependent manner (n = 3). (b) Lower HC030031 concentration significantly reversed the increase of intracellular calcium ions, & 10 μM HC030031 was used in the subsequent experiment (n = 3). (c) Flow cytometry analysis of the control group (PDLCs only, C), L group (PDLCs treated by LPS, L), & LH group (PDLCs treated by 10 μM HC030031 & LPS, LH) (n = 4). (d, e) Western blot analysis & semiquantitative statistical analysis of oxidative stress, apoptosis, & TRPA1 proteins in in C, L, & LH groups (n = 3). (f) EM images showing endoplasmic reticulum (yellow arrows) & mitochondrial morphology (red arrows) of PDLCs in Ctr, LPS, & LH groups (white stars represent cell nuclei) (n = 4). (g) Quantification of endoplasmic reticulum size, mitochondrial size, mitochondrial number per cell (n = 4), & mitochondrial crista density was analyzed (>100 mitochondria). Data analysis was performed by using one-way ANOVA (∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001). Data are presented as the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35720191), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-91319_rabbit-polyclonal-trpa1-antibody-31020241628499.jpg)
![Western Blot: TRPA1 Antibody [NB100-91319] - Periodontitis-derived periodontal ligament cells & tissues were at high levels of oxidative stress & apoptosis. (a) The related gene expressions of oxidative stress, apoptosis, & some of the TRP families in healthy & periodontitis-derived periodontal ligament cells (PDLCs & P-PDLCs) (n = 3). (b, c) Western blot & semiquantitative statistical analysis of oxidative stress, apoptosis, & TRPA1 in PDLCs & P-PDLCs. (n = 3). d, Flow cytometry analysis of PDLCs & P-PDLCs (n = 4). (e, f) H&E staining (white star represent immune cell infiltration), immunohistochemistry & immunofluorescence staining, & semiquantitative statistical analysis of periodontitis & healthy derived periodontal ligament tissues (PDLTs & P-PDLTs) (n = 3). Data analysis was performed by using Student's t-test (∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001). The data are presented as the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35720191), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-91319_rabbit-polyclonal-trpa1-antibody-310202416284922.jpg)
![Western Blot: TRPA1 Antibody (6G8) [H00008989-M03] - Analysis of TRPA1 expression in transfected 293T cell line by TRPA1 monoclonal antibody (M03), clone 6G8. Lane 1: TRPA1 transfected lysate (Predicted MW: 123.09 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TRPA1-Antibody-6G8-Western-Blot-H00008989-M03-img0004.jpg "Western Blot: TRPA1 Antibody (6G8) [H00008989-M03] - Analysis of TRPA1 expression in transfected 293T cell line by TRPA1 monoclonal antibody (M03), clone 6G8. Lane 1: TRPA1 transfected lysate (Predicted MW: 123.09 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: TRPA1 Antibody (6G8) [H00008989-M03] - WB analysis of TRPA1 using anti-TRPA1 antibody on guinea pig nerve ganglia samples. Image from verified customer review.](http://images.novusbio.com/fullsize/TRPA1-Antibody-6G8-Western-Blot-H00008989-M03-img0001.jpg)
![ELISA: TRPA1 Antibody (6G8) [H00008989-M03] - Detection limit for recombinant GST tagged TRPA1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TRPA1-Antibody-6G8-Sandwich-ELISA-H00008989-M03-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRPA1 Antibody [NB100-98841] - IF on vagal nodose ganglia. Image from verified customer review.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98841-img0001.jpg "Immunocytochemistry/Immunofluorescence: TRPA1 Antibody [NB100-98841] - IF on vagal nodose ganglia. Image from verified customer review.")
![Immunohistochemistry: TRPA1 Antibody [NBP3-10359] - Immunohistochemical analysis of rat sensory ganglia tissue](http://images.novusbio.com/fullsize/TRPA1-Antibody-Immunohistochemistry-NBP3-10359-img0001.jpg "Immunohistochemistry: TRPA1 Antibody [NBP3-10359] - Immunohistochemical analysis of rat sensory ganglia tissue")
![SDS-Page: Recombinant Human TRPA1 Protein [H00008989-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00008989-Q01-1.jpg "SDS-Page: Recombinant Human TRPA1 Protein [H00008989-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TRPA1 Antibody [H00008989-D01P] - Analysis of TRPA1 expression in transfected 293T cell line by TRPA1 polyclonal antibody.Lane 1: TRPA1 transfected lysate(123.09 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Western-Blot-H00008989-D01P-img0002.jpg "Western Blot: TRPA1 Antibody [H00008989-D01P] - Analysis of TRPA1 expression in transfected 293T cell line by TRPA1 polyclonal antibody.Lane 1: TRPA1 transfected lysate(123.09 KDa).Lane 2: Non-transfected lysate.")
![ELISA: Human TRPA1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38709] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38709_human-trpa1-ready-to-use-elisa-kit-colorimetric-2110202415384046.png "ELISA: Human TRPA1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38709] - Standard Curve Reference")
![ELISA: Human TRPA1 ELISA Kit (Colorimetric) [NBP3-38710] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38710_human-trpa1-elisa-kit-colorimetric-211020241528263.png "ELISA: Human TRPA1 ELISA Kit (Colorimetric) [NBP3-38710] - Standard Curve Reference")