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![Western Blot: TET1 Antibody [NBP3-11863] - Total protein from human K562 Nuclear and mouse L929 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TET1 (NBP3-11863) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/TET1-Antibody-Western-Blot-NBP3-11863-img0001.jpg "Western Blot: TET1 Antibody [NBP3-11863] - Total protein from human K562 Nuclear and mouse L929 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TET1 (NBP3-11863) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP3-11863] - PC12 cells were fixed for 10 minutes using 4% paraformaldehyde and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TET1 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11863-img0007.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP3-11863] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of TET1 antibody (NBP3-11863). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP3-11863-img0002.jpg)
![Flow (Intracellular): TET1 Antibody [NBP3-11863] - An intracellular stain was performed on NIH3T3 cells with NBP3-11863 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/TET1-Antibody-Flow-Intracellular-NBP3-11863-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP3-11863] - HeLa cells were fixed for 10 minutes using 4% paraformaldehyde and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TET1 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11863-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP3-11863] - NIH3T3 cells were fixed for 10 minutes using 4% paraformaldehyde and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TET1 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11863-img0006.jpg)
![Flow (Intracellular): TET1 Antibody [NBP3-11863] - An intracellular stain was performed on A431 cells with NBP3-11863 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/TET1-Antibody-Flow-Intracellular-NBP3-11863-img0008.jpg)
![Western Blot: TET1 Antibody (1462) [NBP2-15135] - NT2D1 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [1462] diluted at 1:500.](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Western-Blot-NBP2-15135-img0023.jpg "Western Blot: TET1 Antibody (1462) [NBP2-15135] - NT2D1 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [1462] diluted at 1:500.")
![Western Blot: TET1 Antibody (1462) [NBP2-15135] - Representative western blots and densitometry analysis of TET1 levels in untreated or DAC-treated (a) HCT116 monocultures and cocultures with normal BJ or sBJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158986) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Western-Blot-NBP2-15135-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: TET1 Antibody (GT1462) [NBP2-15135] - detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/TET1-Antibody-GT1462-Immunocytochemistry-Immunofluorescence-NBP2-15135-img0011.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135] - Mouse testis. TET1 stained by TET1 antibody [1462] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Immunohistochemistry-Paraffin-NBP2-15135-img0027.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TET1 Antibody (GT1462) [NBP2-15135] - A. 50 ug whole cell lysate/extract from 293T cells transfected with scramble siRNA . B. 50 ug whole cell lysate/extract from TET1-knockdowned 293T cells.](http://images.novusbio.com/fullsize/TET1-Antibody-GT1462-Western-Blot-NBP2-15135-img0013.jpg)
![Western Blot: TET1 Antibody (1462) [NBP2-15135] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells 5 % SDS-PAGE TET1 antibody dilution: 1:500](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Western-Blot-NBP2-15135-img0025.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135] - HeLa xenograft. TET1 antibody [1462] dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Immunohistochemistry-Paraffin-NBP2-15135-img0022.jpg)
![Western Blot: TET1 Antibody (1462) [NBP2-15135] - Changes in the expression of DNMT1 & TET1. Representative western blots & densitometry analysis of DNMT1 & TET1 levels in untreated or DAC-treated (a) HCT116 monocultures & cocultures with normal BJ or sBJ & (b) HCT116 monocultures grown in conditioned medium from normal BJ or sBJ cells. DNMT1 & TET1 blots in (a) & (b) are taken from different gels. Data are mean ± SEM, n = 3, ∗∗∗p < 0.001, ∗p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158986), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15135_mouse-monoclonal-tet1-antibody-1462-31020241538416.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-910202420122065.jpg "Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis. Sample: Paraffin-embedded Human normal prostate tissue. TET1 antibody [N3C1] (NBP2-76345) dilution: 1:1000.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242063370.jpg)
![Western Blot: TET1 Antibody [NBP2-76345] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (NBP2-76345) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242011503.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020241957212.jpg)
![Western Blot: TET1 Antibody [NBP2-76345] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (NBP2-76345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-910202420104413.jpg)
![Immunohistochemistry: TET1 Antibody [NBP2-76345] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (NBP2-76345) antibody at 1:1000 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242014666.jpg)
![Western Blot: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein by western blot analysis.A. 30 ug 293T whole cell lysate/extractB. 30 ug whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells5% SDS-PAGETET1 antibody [N3C1] (NBP2-76345) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242014642.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242021956.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . TET1 antibody [N3C1] (NBP2-76345) diluted at 1:250.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242091468.jpg)
![Immunocytochemistry/ Immunofluorescence: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242041232.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TET1 Antibody [NBP2-76345] - Non-transfected (–) and transfected (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-1610202419414597.jpg)
![Western Blot: TET1 Antibody [NBP2-19290] - WB analysis of lysates from 293T cell which were mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either TET1 antibody (diluted at 1:2000) (A) or anti-tag (B) antibody.](http://images.novusbio.com/fullsize/TET1-Antibody-Western-Blot-NBP2-19290-img0003.jpg "Western Blot: TET1 Antibody [NBP2-19290] - WB analysis of lysates from 293T cell which were mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either TET1 antibody (diluted at 1:2000) (A) or anti-tag (B) antibody.")
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP2-19290] - ICC-IF validation of TET1 antibody on HepG2 cells which were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET1 protein stained by TET1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19290-img0002.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-19290] - Mouse testis. TET1 stained by TET1 antibody [N1], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP2-19290-img0004.jpg)
![Western Blot: TET1 Antibody [NBP2-19290] - WB validation of TET1 antibody on 30 ug whole cell extracts of non-transfected (-) and mouse TET1-transfected (+, including DDDDK-tag) 293T cells. The proteins were separated on 5% SDS-PAGE, and the membrane was blotted with TET1 antibody at a dilution of 1:20000.](http://images.novusbio.com/fullsize/TET1-Antibody-Western-Blot-NBP2-19290-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP1-88972] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88972-img0005.jpg "Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP1-88972] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.")
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0009.jpg)
![Immunohistochemistry: TET1 Antibody [NBP1-88972] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-NBP1-88972-img0003.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human cerebellum shows moderate nuclear positivity in purkinje cells.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0006.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0007.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0008.jpg)
![Immunohistochemistry: TET1 Antibody [NBP1-78966] - Analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-NBP1-78966-img0001.jpg "Immunohistochemistry: TET1 Antibody [NBP1-78966] - Analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.")
![Immunohistochemistry: TET1 Antibody [NBP1-78965] - IHC analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-NBP1-78965-img0001.jpg "Immunohistochemistry: TET1 Antibody [NBP1-78965] - IHC analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.")
![Western Blot: TET1 Antibody (GT2457) - Azide and BSA Free [NBP3-25717] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT2457] (NBP3-25717) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25717_mouse-tet1-mab-gt2457-azide-and-bsa-free-2120249371593.jpg "Western Blot: TET1 Antibody (GT2457) - Azide and BSA Free [NBP3-25717] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT2457] (NBP3-25717) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: TET1 Antibody (GT2457) - Azide and BSA Free [NBP3-25717] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT2457] (NBP3-25717) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25717_mouse-tet1-mab-gt2457-azide-and-bsa-free-212024941482.jpg)
![Knockdown Validated: TET1 Antibody (GT231) - Azide and BSA Free [NBP3-25716] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT231] (NBP3-25716) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25716_mouse-tet1-mab-gt231-azide-and-bsa-free-2120249432891.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: TET1 Antibody (GT231) - Azide and BSA Free [NBP3-25716] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT231] (NBP3-25716) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: TET1 Antibody (GT231) - Azide and BSA Free [NBP3-25716] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT231] (NBP3-25716) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25716_mouse-tet1-mab-gt231-azide-and-bsa-free-2120249413340.jpg)
![Western Blot: TET1 Antibody (GT1382) - Azide and BSA Free [NBP3-25715] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1382] (NBP3-25715) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25715_mouse-tet1-mab-gt1382-azide-and-bsa-free-2120249374944.jpg "Western Blot: TET1 Antibody (GT1382) - Azide and BSA Free [NBP3-25715] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1382] (NBP3-25715) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: TET1 Antibody (GT1382) - Azide and BSA Free [NBP3-25715] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1382] (NBP3-25715) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25715_mouse-tet1-mab-gt1382-azide-and-bsa-free-212024940871.jpg)